Fluorescence Imaging Technique for Detection of Human Melanoma

Chwirot, Barbara W.; Chwirot, S.; S£owikowska, K.; Redzinski, Jaroslaw; Tazbir, J.; Sir, Jan; Nussbeutel, Joanna; Grądziel, M.

doi:10.1007/978-94-017-0928-6_14

Cited by 2 publications

(2 citation statements)

References 10 publications

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“…Method The ¯uorescence method for in situ detection of melanoma of the skin was described in detail in our previous publications (Chwirot et al, 1998a;1998b). Brie¯y, it consists in digital imaging of the auto¯uorescence of pigmented lesions and of the skin excited with the 366 nm UVA line of a high-pressure Xe±Hg lamp (Hamamatsu, Japan) and emitted in the blue 475 nm band.…”

Section: Methodsmentioning

confidence: 99%

Fluorescence In Situ Detection of Human Cutaneous Melanoma: Study of Diagnostic Parameters of the Method

Chwirot

Sypniewska

et al. 2001

Journal of Investigative Dermatology

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Multicenter study of the diagnostic parameters was conducted by three groups in Poland to determine if in situ fluorescence detection of human cutaneous melanoma based on digital imaging of spectrally resolved autofluorescence can be used as a tool for a preliminary selection of patients at increased risk of the disease. Fluorescence examinations were performed for 7228 pigmented lesions in 4079 subjects. Histopathologic examinations showed 56 cases of melanoma. A sensitivity of fluorescence detection of melanoma was 82.7% in agreement with 82.5% found in earlier work. Using as a reference only the results of histopathologic examinations obtained for 568 cases we found a specificity of 59.9% and a positive predictive value of 17.5% (melanomas versus all pigmented lesions) or 24% (melanomas versus common and dysplastic naevi). The specificity and positive predictive value found in this work are significantly lower than reported earlier but still comparable with those reported for typical screening programs. In conclusion, the fluorescence method of in situ detection of melanoma can be used in screening large populations of patients for a selection of patients who should be examined by specialists.

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Section: Methodsmentioning

confidence: 99%

Fluorescence In Situ Detection of Human Cutaneous Melanoma: Study of Diagnostic Parameters of the Method

Chwirot

Sypniewska

et al. 2001

Journal of Investigative Dermatology

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“…In contrast, the pathological process may affect their concentration, mutual relation, and the change in the local microenvironment, which can be manifested by altered fluorescence. Autofluorescence of biological systems is a phenomenon with huge potential in non-invasive diagnostics of pathological states [1,2] 3D fluorescence analysis in various graphic realizations, mainly in the form of an excitation-emission matrix (EEM), allows the description and visualization of the composition of the fluorescent metabolome of a given biological system. Thus, comparing the analyzed sample with a defined standard clearly identifies differences caused by qualitative or quantitative changes in compositions in response to a pathological process.…”

Section: Introductionmentioning

confidence: 99%

Visualization of the composition of the urinary fluorescent metabolome. Why is it important to consider initial urine concentration?

Dubayová

Birková

Leso

et al. 2023

Methods Appl. Fluoresc.

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Urine is a highly complex fluorescent system, the fluorescence of which can be affected by many factors, including the often-ignored initial urine concentration in comprehensive fluorescent urine analysis. In this study, a total urine fluorescent metabolome profile (uTFMP) was created as a three-dimensional fluorescence profile of serial synchronous spectra of urine diluted by geometric progression. uTFMP was generated using software designed for this purpose after recalculating the 3D data concerning the initial urine concentration. It can be presented as a contour map (top view) or as a more illustrative and straightforward simple curve, thus usable in various medicinal applications.

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Fluorescence Imaging Technique for Detection of Human Melanoma

Cited by 2 publications

References 10 publications

Fluorescence In Situ Detection of Human Cutaneous Melanoma: Study of Diagnostic Parameters of the Method

Fluorescence In Situ Detection of Human Cutaneous Melanoma: Study of Diagnostic Parameters of the Method

Visualization of the composition of the urinary fluorescent metabolome. Why is it important to consider initial urine concentration?

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