Fluorescence imaging with tailored light

Tang, Jialei; Ren, Jinhan; Han, Kyu Young

doi:10.1515/nanoph-2019-0227

Cited by 38 publications

(28 citation statements)

References 232 publications

(249 reference statements)

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“…Fluorescence microscopy is known as a fundamental set of methods for visualizing stained structures in the system under various conditions [1]. Fluorescence microscopy is generally used in the life sciences, but some studies reveal that new techniques could achieve greater characterization of the material without being a living system [2]. Normally, a biological sample can be stained with a fluorescent molecule, either synthetic or biological, and subsequently, a structure of interest is observed in more detail or definition [2,3].…”

Section: Main Textmentioning

confidence: 99%

“…Fluorescence microscopy is generally used in the life sciences, but some studies reveal that new techniques could achieve greater characterization of the material without being a living system [2]. Normally, a biological sample can be stained with a fluorescent molecule, either synthetic or biological, and subsequently, a structure of interest is observed in more detail or definition [2,3]. Fluorescent molecules can be visualized based on two main factors: sample preparation and the targets used, but the resolution at which samples can be observed varies according to technique and may be limited by unfocused light capture in a focal plane or an evanescence plane [4,5].…”

Section: Main Textmentioning

confidence: 99%

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CLSM and TIRF images from lignocellulosic materials: garlic skin and agave fibers study

et al. 2021

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Fluorescence techniques have been widely used by scientists to reveal valuable information from biological samples, but in food science, small progress is known due to the complexity of the samples. In this study, two different biological samples, garlic skin (GS) and agave fibers (AF), were used to evaluate the techniques of confocal laser scanning microscopy (CLSM) and total internal reflection fluorescence (TIRF) microscopy, to obtain valuable information on the fiber size of the samples. A compositional characterization with calcofluor white in CLSM was achieved, but a superficial characterization of the samples with TIRF was made, evidencing fiber sizes of 398.67 ± 48.47 nm and 677.38 ± 76.88 nm for GS and AF, respectively. This work reveals that only an untreated sample can be used with the two techniques in the same microscope. In addition, it is possible to characterize the sample only using a spatial field of research and which valuable information about the structure of the material is found. This work provides the opportunity to use advanced fluorescence techniques for elucidation of structures shortly before studied with these techniques.

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Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

CLSM and TIRF images from lignocellulosic materials: garlic skin and agave fibers study

et al. 2021

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“…Yield: 81%. 1 35 mmol) were dissolved with 2-ethoxyethanol and water (40 mL, 3:1, v:v). Then, the mixture was heated at 110 • C under N 2 atmosphere for 1 day.…”

Section: Synthesis 211 Synthesis Of Ligandmentioning

confidence: 99%

“…Optical molecular imaging technology is an extraordinary tool that can provide noninvasive, high-accuracy and high-resolution optical information in biomedical areas [1][2][3][4]. In order to stimulate the progress of this technology, the most important point is to promote innovations of higher-performance molecular probes [5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Deep NIR-I Emissive Iridium(III) Complex Bearing D-A Ligand: Synthesis, Photophysical Properties and DFT/TDDFT Calculation

Jiang

et al. 2021

Crystals

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Near-infrared (NIR) phosphorescent iridium(III) complexes have been demonstrated to possess photophysical properties superior to those of traditional NIR dyes. However, the NIR emission wavelength is restricted in the range of 700–800 nm. For realizing deeper NIR emission, a novel type of iridium(III) complex was designed and synthesized in this work. The main ligand of the iridium(III) complex was constructed using a donor-acceptor structure containing benzothiophene as the donor and quinoxaline as the acceptor. The β-diketone derivative was chosen as the auxiliary ligand owing to its symmetrical structure and p-donating character. The complex exhibits deep NIR-I phosphorescence (764 nm in CH2Cl2, 811 nm in aqueous solution) and broad full width at half maximum (108 nm in CH2Cl2, 154 nm in aqueous solution). Theoretical calculations based on the density function and time-dependent density function were carried out to support the experimental data. Moreover, in vitro biological performance of the complex was determined as well. This work supports the possibility that via a systematic transformation between the D and A units, the photophysical performance of NIR emissive iridium(III) complexes can be greatly improved.

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“…The LLSM developed by Eric Betzig and his collaborators exploits a thin light-sheet with lattice patterns for illumination and is regarded as an advanced scheme of LSFM [10,11]. Compared to other types of light-sheet illumination, the lattice light-sheet enables simultaneous optimization of axial resolution (influenced by the thickness of the light-sheet), the field of view (the effective illumination area of the light-sheet) and background noise caused by out-of-focus fluorescence excitation [12,13]. However, there are two major drawbacks of LLSM from our point of view: expensive and complicated optical setups, as well as the inevitable trade-off between a large field of view and high light throughput.…”

Section: Introductionmentioning

confidence: 99%

High-efficiency, large-area lattice light-sheet generation by dielectric metasurfaces

2020

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AbstractLattice light-sheet microscopy (LLSM) was developed for long-term live-cell imaging with ultra-fine three-dimensional (3D) spatial resolution, high temporal resolution, and low photo-toxicity by illuminating the sample with a thin lattice-like light-sheet. Currently available schemes for generating thin lattice light-sheets often require complex optical designs. Meanwhile, limited by the bulky objective lens and optical components, the light throughput of existing LLSM systems is rather low. To circumvent the above problems, we utilize a dielectric metasurface of a single footprint to replace the conventional illumination modules used in the conventional LLSM and generate a lattice light-sheet with a ~3-fold broader illumination area and a significantly leveraged illumination efficiency, which consequently leads to a larger field of view with a higher temporal resolution at no extra cost of the spatial resolution. We demonstrate that the metasurface can manipulate spatial frequencies of an input laser beam in orthogonal directions independently to break the trade-off between the field of view and illumination efficiency of the lattice light-sheet. Compared to the conventional LLSM, our metasurface module serving as an ultra-compact illumination component for LLSM at an ease will potentially enable a finer spatial resolution with a larger numerical-aperture detection objective lens.

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Fluorescence imaging with tailored light

Cited by 38 publications

References 232 publications

CLSM and TIRF images from lignocellulosic materials: garlic skin and agave fibers study

CLSM and TIRF images from lignocellulosic materials: garlic skin and agave fibers study

Deep NIR-I Emissive Iridium(III) Complex Bearing D-A Ligand: Synthesis, Photophysical Properties and DFT/TDDFT Calculation

High-efficiency, large-area lattice light-sheet generation by dielectric metasurfaces

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