Fluorescence immunosensor based on functional nanomaterials and its application in tumor biomarker detection

Huang, Juanjuan; Wei, Fenghuang; Cui, Yuling; Hou, Li; Lin, Tianran

doi:10.1039/d2ra04989a

Cited by 17 publications

(5 citation statements)

References 98 publications

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“…Fluorescence assays have attracted much attention because of their high sensitivity, simple operation, and fast response. A variety of molecules and nanomaterials can be used as fluorescent probes or substrates, such as fluorescein isothiocyanate, semiconductor quantum dots, noble metal nanoclusters, and carbon dots [ 66 ]. AA can serve as a reducing agent to be electrochemically or chemically oxidized into DHA [ 67 ].…”

Section: Redox Cycling-based Optical Bioassaysmentioning

confidence: 99%

Optical Bioassays Based on the Signal Amplification of Redox Cycling

Feng,

Gao,

et al. 2024

Biosensors

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Optical bioassays are challenged by the growing requirements of sensitivity and simplicity. Recent developments in the combination of redox cycling with different optical methods for signal amplification have proven to have tremendous potential for improving analytical performances. In this review, we summarized the advances in optical bioassays based on the signal amplification of redox cycling, including colorimetry, fluorescence, surface-enhanced Raman scattering, chemiluminescence, and electrochemiluminescence. Furthermore, this review highlighted the general principles to effectively couple redox cycling with optical bioassays, and particular attention was focused on current challenges and future opportunities.

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Section: Redox Cycling-based Optical Bioassaysmentioning

confidence: 99%

Optical Bioassays Based on the Signal Amplification of Redox Cycling

Feng,

Gao,

et al. 2024

Biosensors

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“…Immunosensors, which refer to the detection of data obtained as a result of immunochemical reactions through a transducer, are analytical devices that monitor the specific binding of antibodies and antigens to determine biomolecules, such as biomarkers, bacteria, viruses, and many other small molecules [1,2]. When this biological reaction mechanism used for sensing is integrated with the features of the electrochemical transducer such as miniaturization, multiple analysis, cheap and fast detection, it enables the creation of more practical and versatile products [3].…”

Section: Introductionmentioning

confidence: 99%

The role of nanoparticles in non‐invasive electrochemical immunosensor technology and recent developments

Sanko,

Erkmen,

Kuralay

2024

Electroanalysis

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“…In contrast to electronic biosensors, they offer high sensitivity and selectivity, as well as stability and the ability to detect and quantify a wide range of targets. Fluorescence-based biosensors also have a wide dynamic range, enabling the detection of low-abundance targets in complex biological samples 32,33 . Another advantage of fluorescence biosensors is their compatibility with microfabrication techniques, which allows for the development of miniaturized devices for point-of-care and field-based applications with a simple operation.…”

Section: Introductionmentioning

confidence: 99%

“…The SiNPs provides several advantageous features as a sensing area for biosensing applications and especially immunosensors. For example, a high surface area-to-volume ratio renders them highly sensitive to changes in the local environment, a crucial characteristic for detecting small amounts of analytes and enhancing the resulting signal 32,[40][41][42] .…”

Section: Introductionmentioning

confidence: 99%

Transdermal Minimally-Invasive Optical Multiplex Detection of Protein Biomarkers by Microneedles-Embedded Nanopillars Array

Raz,

Gubi,

Cohen

et al. 2024

Preprint

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Biomarkers detection have become essential in medical diagnostics and early detection of life-threatening diseases. Modern-day medicine relies heavily on painful and invasive tests, the extraction of large volumes of venous blood being the most common tool of biomarker detection. These tests are time-consuming, expansive, and require complex sample manipulations and trained staff. The application of 'intradermal' biosensors utilizing microneedles as a minimally-invasive and pain-free sampling and sensing elements for capillary blood biomarkers detection has gained extensive interest in the past few years as a central point-of-care (POC) detection platform. Herein, we present a new diagnosis paradigm based on vertically-aligned nanopillars array-embedded microneedles sampling-and-detection elements for the direct optical detection and quantification of biomarkers in capillary blood. We present here the first demonstration on the simple fabrication route for the creation of a multidetection-zone silicon nanopillars array, embedded in microneedle elements, followed by their area-selective chemical modification, towards the multiplex intradermal biomarkers detection. The utilization of the rapid and specific antibody-antigen binding, combined with the intrinsically large sensing area created by the nanopillars array, enables the simultaneous efficient ultrafast and highly sensitive intradermal capillary blood sampling and detection of protein biomarkers of clinical relevance, without requiring the extraction of blood samples for the ex-vivo biomarkers analysis. Through preliminary in vitro and in vivo experiments, the direct intradermal in-skin blood extraction-free platform has demonstrated a remarkable sensitivity (low pM) and specificity for the accurate multiplex detection of protein biomarkers in capillary blood.

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Fluorescence immunosensor based on functional nanomaterials and its application in tumor biomarker detection

Abstract: Fluorescence immunosensors constructed from functional nanomaterials for the detection of tumor biomarkers such as enzymes, RNA, DNA, proteins and cancer cells were summarized.

Cited by 17 publications

References 98 publications

Optical Bioassays Based on the Signal Amplification of Redox Cycling

Optical Bioassays Based on the Signal Amplification of Redox Cycling

The role of nanoparticles in non‐invasive electrochemical immunosensor technology and recent developments

Transdermal Minimally-Invasive Optical Multiplex Detection of Protein Biomarkers by Microneedles-Embedded Nanopillars Array

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