Fluorescence in situ hybridization analysis of nitrifiers in piggery wastewater treatment reactors

Abstract

Fluorescence in situ hybridization (FISH) was performed to analyze the nitrifying microbial communities in an activated sludge reactor (ASR) and a fixed biofilm reactor (FBR) for piggery wastewater treatment. Heterotrophic oxidation and nitrification were occurring simultaneously in the ASR and the COD and nitrification efficiencies depend on the loads. In the FBR nitrification efficiency also depends on ammonium load to the reactor and nitrite was accumulated when free ammonia concentration was higher than 0.2 mg NH3-N/L. FISH analysis showed that ammonia-oxidizing bacteria (NSO1225) and denitrifying bacteria (RRP1088) were less abundant than other bacteria (EUB338) in ASR. Further analysis on nitrifying bacteria in the FBR showed that Nitrosomonas species (NSM156) and Nitrospira species (NSR1156) were the dominant ammonia-oxidizing and nitrite-oxidizing bacteria, respectively, in the piggery wastewater nitrification system.

Molecular ecology of a facultative swine waste lagoon

“…1992) (see Table 1) and compared to input waste from Charlie Taylor’s Piggery – Redbank. All sample fixation and in situ hybridization reactions were performed with some modifications to the published protocol (Kim et al. 2004).…”

“…Fluorescence in situ hybridization was conducted on samples from all three swine waste lagoons (P&J Taylor Piggery -Gayndah, Charlie Taylor's Piggery -Redbank, Department of Primary Industries and Fisheries Piggery -Wacol, all located in Greater Brisbane, Queensland, Australia) using published oligonucleotide probes: EUBmix (EUB338, EUB338-II and EUB338-III) (Amann et al 1990), ALF969 (Siyambalapitiya and Blackall 2005) and GAM42a (Manz et al 1992) (see Table 1) and compared to input waste from Charlie Taylor's Piggery -Redbank. All sample fixation and in situ hybridization reactions were performed with some modifications to the published protocol (Kim et al 2004). A formamide concentration of 35% (v ⁄ v) was used for hybridization.…”

Sequencing Batch Reactor and Bacterial Community in Aerobic Granular Sludge for Wastewater Treatment of Noodle-Manufacturing Sector

“…It is known that culture-dependent methods have limitations for studying natural microbial community composition, because only a small part of bacteria in environmental samples are culturable under laboratory conditions [8]. Molecular techniques such as polymerase chain reaction-denaturing gradient gel electrophoresis) (PCR-DGGE) [9][10][11], amplified ribosomal DNA restriction analyses (ARDRA) [12], and fluorescence in situ hybridization (FISH) have been widely applied to study the diversity of microorganisms in natural samples [13]. These techniques have significantly overcome the weakness of traditional methods, but only elucidate the major microbial groups in wastewater treatments.…”

N-removal performance and underlying bacterial taxa of upflow filter bioreactor system under different dissolved oxygen and internal recycle conditions

“…Nitrite-oxidizing bacteria (NOB) were extensively studied in terms of their inhibition or elimination to avoid nitrification-denitrification [6][7][8][9][10], while ammoniaoxidizing bacteria (AOB) underlying the nitritation process were also investigated [11][12][13][14]. Denitrifiers in general received a considerable attention as a part of the process of denitrification, although their role in the denitritation process was not elucidated.…”