Fluorescence in situ hybridization-based approaches for detection of 12p overrepresentation, in particular i(12p), in cell lines of human testicular germ cell tumors of adults

Mostert, Marijke; Pol, Marjolein van de; Echten, Jannie van; Weghuis, D.E.M. Olde; Kessel, A.H.M. Geurts van; Oosterhuis, J. Wolter; Looijenga, Leendert

doi:10.1016/0165-4608(95)00233-2

Cited by 33 publications

(17 citation statements)

References 34 publications

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“…The strict criteria adapted in the current study assured high specificity for the identification of i(12p), but this may also contribute to the limited number of cells being recognized as i(12p)-positive tumor cells. Nonetheless, the percentage of i(12p)-positive cells (2-5%) reported here in mature teratoma is comparable to the percentages reported in the literature cases (32,35,36). Because the percentage of cells with recognizable i(12p) signals in germ cell tumors is typically quite low (2-5%), tissues with cell types comparable with those in the studied lesion (e.g., normal squamous epithelium) would serve as optimal controls.…”

Section: Discussionsupporting

confidence: 62%

Interphase Fluorescence In situ Hybridization Analysis of Chromosome 12p Abnormalities Is Useful for Distinguishing Epidermoid Cysts of the Testis from Pure Mature Teratoma

Liu

Zhang²,

MacLennan

et al. 2006

Clinical Cancer Research

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Purpose: The distinction of epidermoid cyst of the testis from teratoma is of critical importance because the former is benign and the latter is a malignant tumor that may have associated metastasis of either teratomatous or non^teratomatous germ cell tumor types. Chromosome 12p abnormalities are seen in the vast majority of testicular germ cell tumors of adults and are present in all histologic subtypes. In this study, we investigated the clinical utility of interphase fluorescence in situ hybridization (FISH) analysis of chromosome 12p abnormalities for distinguishing epidermoid cysts of the testis from pure mature teratoma. Experimental Design: Sixteen testicular epidermoid cysts and 17 testicular teratomas were investigated for isochromosome 12p [i(12p)] and 12p overrepresentation using interphase FISH analysis. Results: Neither i(12p) nor 12p overrepresentation were observed in 16 epidermoid cyst cases, whereas i(12p) was detected in 76% of teratomas and 12p overrepresentation was identified in 29% of teratomas. Overall, 88% of testicular teratomas had chromosome 12p abnormalities. Conclusions: FISH identification of i(12p) and/or 12p overrepresentation in routinely processed surgical specimens is a useful ancillary diagnostic tool in distinguishing testicular epidermoid cysts from teratoma.

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Section: Discussionsupporting

confidence: 62%

Interphase Fluorescence In situ Hybridization Analysis of Chromosome 12p Abnormalities Is Useful for Distinguishing Epidermoid Cysts of the Testis from Pure Mature Teratoma

Liu

Zhang²,

MacLennan

et al. 2006

Clinical Cancer Research

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“…Amplification at 1q was also found to be common in previous CGH studies of other solid tumors [Forozan et al, 1997;Knuutila et al, 1998]. Gain of 12p was also noted in breast tumors, testicular germ cell tumors, pancreatic tumors, and leukemias Mostert et al, 1996;Mahlamaki et al, 1997;Willem and Mendelow, 1997].…”

Section: Genomic Gains In Npcmentioning

confidence: 58%

Chromosomal aberrations in nasopharyngeal carcinoma analyzed by comparative genomic hybridization

Chen¹,

Chen

et al. 1999

Genes Chromosom. Cancer

101

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“…Human GCT are characterised by the frequent amplification of the short arm of chromosome 12 (12p), usually in the form of one or more isochromosomes (12p) (Atkin and Baker, 1982;Mostert et al, 1996;Sandberg et al, 1996), and of the long arm of chromosome 17 (17q) (Kraggerud et al, 2002). A number of candidate genes that might play a role in this selection have been identified on chromosomes 17q and 12p.…”

Section: Introductionmentioning

confidence: 99%

The CDK inhibitor p27 enhances neural differentiation in pluripotent NTERA2 human EC cells, but does not permit differentiation of 2102Ep nullipotent human EC cells

Bahrami

Matin

Andrews

2005

Mechanisms of Development

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Embryonal carcinoma (EC) cells, the stem cells of teratocarcinomas, are the malignant counterparts of pluripotent embryonic stem (ES) cells, but commonly exhibit a reduced ability to differentiate, presumably because of continual selection for genetic changes that alter the balance between self-renewal, differentiation and apoptosis in favour of self-renewal. To explore the nature of the genetic changes that promote nullipotency, we have compared two human EC cell lines, a 'nullipotent' line, 2102Ep, and a 'pluripotent' line, NTERA2. A hybrid derived by fusion of these cells differentiates in response to retinoic acid but, unlike the parental NTERA2 line, does not form terminally differentiated neurons. This implies that the nullipotent EC cell line, 2102Ep, differs in expression of at least two functions in comparison with the NTERA2 pluripotent line, one affecting commitment to differentiation, and one affecting terminal neural differentiation. We have now investigated the possible role of the CDK inhibitor, p27kip1 (p27) in commitment and terminal differentiation. In NTERA2, but not in 2102Ep cells, retinoic acid induces up-regulation of p27 expression, suggesting that 2102Ep cells lack this capacity. However, constitutive expression of a p27 transgene does not overcome the block to differentiation in the 2102Ep parental cells; commitment to differentiation must be blocked elsewhere. On the other hand, constitutive over-expression of p27 from a transgene enhances the neural differentiation of NTERA2 cells. Our results suggest that p27 plays a role in terminal neuronal differentiation of human EC cells, but not in their initial commitment to differentiation, and that other factors, possibly Cyclin D2, specifically limit its ability to promote neural differentiation.

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Fluorescence in situ hybridization-based approaches for detection of 12p overrepresentation, in particular i(12p), in cell lines of human testicular germ cell tumors of adults

Cited by 33 publications

References 34 publications

Interphase Fluorescence In situ Hybridization Analysis of Chromosome 12p Abnormalities Is Useful for Distinguishing Epidermoid Cysts of the Testis from Pure Mature Teratoma

Interphase Fluorescence In situ Hybridization Analysis of Chromosome 12p Abnormalities Is Useful for Distinguishing Epidermoid Cysts of the Testis from Pure Mature Teratoma

Chromosomal aberrations in nasopharyngeal carcinoma analyzed by comparative genomic hybridization

The CDK inhibitor p27 enhances neural differentiation in pluripotent NTERA2 human EC cells, but does not permit differentiation of 2102Ep nullipotent human EC cells

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