Fluorescence in situ hybridization deletion mapping at 4p16.3 in bladder cancer cell lines refines the localisation of the critical interval to 30 kb

Allelic changes at multiple regions of chromosome 5 are associated with progression of urinary bladder cancer

“…Only a few tumour suppressor gene loci are narrowed to an appropriate size which would allow a positional cloning of new genes in bladder cancers20–22. We have now identified three regions of interest on chromosome 5, one of them within aproximately 4 cM genetic distance at chromosome 5q23.…”

Redefining a critical region of LOH on 4p16.3 in bladder cancer

“…The combination of these two factors despite the use of control hybridizations in all analyses led to the Bell et al (1996). In our experience, we have found that the quality of metaphase spreads from different cell types can give a false impression of hybridization efficiency.…”

“…We previously reported deletion of band 4p16.3 in bladder cancer, identified by both LOH analysis on primary tumors (Elder et al, 1994) and FISH analysis on tumor cell lines (Bell et al, 1996). The former study pinpointed a minimum region of deletion of approximately 750 kb between the markers D4S43 and D4S127.…”

Genetic initiation, progression and prognostic markers in transitional cell carcinoma of the bladder: a summary of the structural and transcriptional changes, and the role of developmental genes

“…About 20% of bladder tumours exhibit LOH on 4p [ 63, 64]. There is accumulating evidence that deletions on chromosome 4 contribute to late progression in bladder cancer and although any putative TSG has yet to be identified, the candidate region has been narrowed down to 30 kb at 4p16.3 [ 65]. Deletions in 8p are found in ≈20% of bladder tumours and there is a strong correlation with high stage and grade; indeed, 53% of grade 3, muscle‐invasive tumours had deletions, compared with 11% of grade 1 non‐invasive tumours [ 66].…”