Fluorescence in situ hybridization for the detection and monitoring of the Ph-positive clone in chronic myelogenous leukemia: comparison with metaphase banding analysis

Comparison of chromosome banding analysis, interphase- and hypermetaphase-FISH, qualitative and quantitative PCR for diagnosis and for follow-up in chronic myeloid leukemia: a study on 350 cases

“…This was predominately observed in cases with BCR-ABL positivity Ͼ30%. Therefore, we cannot confirm the data of Cuneo et al, 22 who observed no differences in BCR-ABL positivity between IP-FISH performed on directly harvested or cultured cells. As differences in cultivation conditions have an impact on the results, a standardization of methods especially within clinical trials is needed to allow the comparison of treatment results.…”

“…Several studies showed that results of interphase-FISH performed on blood cells tracked the corresponding percentages of BCR-ABL-positive interphase nuclei of bone marrow cells determined by FISH and of Ph + metaphases. [22][23][24][25] Therefore, interphase-FISH performed on blood cells seems to be an appropriate method to monitor response to treatment. In our study the cut-off level for interphase-FISH was set at 5%.…”

Chronic myelogenous leukemia: Laboratory diagnosis and monitoring

“…Recent studies performed to evaluate cytogenetic and FISH quantification have shown an excellent correlation between the two assays (Cuneo et al, 1998;Glassman, 1998;Kobzev et al, 1998). As noted earlier, FISH is more sensitive than cytogenetics in detecting Ph/BCR-ABL-positive cells in patients in whom the leukemic burden is low (Cuneo et al, 1998). For these reasons, we strongly recommend that cytogenetics be replaced by FISH for monitoring of IFN␣ therapy.…”

“…In addition, a FISH study routinely analyses 200 to 500 nuclei; thus, quantification generated by FISH is more accurate than cytogenetics, especially when few leukemic cells are present, as is frequently seen posttherapy. In one study correlating cytogenetics and FISH, FISH detected 2.5-8% BCR-ABL-positive cells in seven of nine specimens, from six patients, in which cytogenetic results were negative (Cuneo et al, 1998). Because of the added accuracy and sensitivity, FISH is being used increasingly to replace cytogenetics in monitoring of patients on IFN␣ and newer biological or chemotherapies (Buno et al, 1998;Duba et al, 1999).…”

Towards the Cure of CML by the Molecular Approach Strategy

“…Interphase FISH is applicable to a larger population of cells since does not require cycling cells. On the other hand, this technique is associated with a background signal greater than 1-5% (depending on the specific probe used in the assay) (Cuneo et al, 1998;Le Gouill et al, 2000;Lesser et al, 2002;Raanani et al, 2004). Hypermetaphase FISH is applicable only to dividing bone marrow cells (Schoch et al, 2002).…”