Fluorescence intensity profiles of in situ hybridization signals depict genome architecture within human interphase nuclei

Iourov, Ivan Y.; Sg, Vorsanova; Yurov, Yuri B.

doi:10.3103/s0095452708050010

Cited by 4 publications

(9 citation statements)

References 13 publications

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“…In this context, it is important to bear in mind that IQ-FISH offers a unique possibility to demonstrate accurately the presence chromosomal heteromorphisms in interphase nuclei. As we noted previously [14], QFISH provides for monitoring denature time and duration of hybridization to identify optimal hybridization conditions. It was established that 100% efficiency of FISH (i.e.…”

Section: Methodsmentioning

confidence: 89%

“…Additionally, IQ-FISH using DNA probes for repetitive genomic elements highlights the intranuclear arrangements of chromosomal DNAs (i.e. distribution of satellite DNA within a nuclear area) [14]. More importantly, IQ-FISH seems to be the way of uncovering chromosomal associations in almost any FISH study of interphase nuclei.…”

Section: Applicationsmentioning

confidence: 99%

“…Similarly, high-resolution FISH-based techniques for multicolor painting of interphase chromosomes involve signal quantification [12,13]. Finally, FISH-signal intensity profiles are able to depict genome architecture within human interphase nuclei [14]. Interestingly, QFISH has been demonstrated to be valuable for molecular cytogenetic diagnosis [4,[15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Here, we describe a combination of original interphase FISH protocols [1,7,9,12] with a QFISH approach [3,4,14]. The combination has been termed interphase QFISH (IQ-FISH).…”

Section: Introductionmentioning

confidence: 99%

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Interphase Quantitative Fluorescence in Situ Hybridization (IQ-FISH)

Iourov¹,

Soloviev²,

Yurov³

et al. 2019

obm genet

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Fluorescence in situ hybridization (FISH) seems to be the most applicable and versatile molecular cytogenetic platform for visual interphase chromosome analysis offering a variety of opportunities for studying chromosomal structure and behaviour at the highest microscopic resolution and at all stages of the cell cycle. Quantitative assessment of FISH results has been repeatedly shown to increase the efficiency of FISH-based approaches. Here, we explore the potential of interphase quantitative FISH (IQ-FISH), which is the result of combining interphase FISH with an approach to FISH signal quantification. Firstly, IQ-FISH is applicable for high-resolution molecular cytogenetic analysis of somatic genome variations in large populations of interphase cells (n>1000) inasmuch as it allows for the differentiation between genomic variations (chromosome abnormalities) and specific nuclear chromosomal organization. Secondly, IQ-FISH may be a basis for more sophisticated interphase molecular cytogenetic techniques (i.e. multicolour banding of interphase chromosomes). Thirdly, IQ-FISH depicts nuclear organization by demonstrating the distribution of specific chromosomal DNA within nuclear area. Finally, IQ-FISH is a unique technique allowing quantitative

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Section: Methodsmentioning

confidence: 89%

Section: Applicationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Here, we describe a combination of original interphase FISH protocols [1,7,9,12] with a QFISH approach [3,4,14]. The combination has been termed interphase QFISH (IQ-FISH).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Interphase Quantitative Fluorescence in Situ Hybridization (IQ-FISH)

Iourov¹,

Soloviev²,

Yurov³

et al. 2019

obm genet

View full text Add to dashboard Cite

show abstract

“…The latter was found applicable for analysis of chromosomal heteromorphisms/pericentromeric regions (i.e. identification of parental origins of homologous chromosomes with efficiency comparable to PCR-based methods) [ 72 ], distinguishing between interphase chromosome loci pairing or associations and chromosomal loss [ 73 ], and determining specific genome architecture within human interphase nuclei [ 74 ]. Still, the possibility to see an interphase chromosome in its integrity was not available.…”

mentioning

confidence: 99%

Yuri B. Yurov (1951-2017)

Iourov

Vorsanova

2018

Mol Cytogenet

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Human interphase chromosomes: a review of available molecular cytogenetic technologies

2010

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Human karyotype is usually studied by classical cytogenetic (banding) techniques. To perform it, one has to obtain metaphase chromosomes of mitotic cells. This leads to the impossibility of analyzing all the cell types, to moderate cell scoring, and to the extrapolation of cytogenetic data retrieved from a couple of tens of mitotic cells to the whole organism, suggesting that all the remaining cells possess these genomes. However, this is far from being the case inasmuch as chromosome abnormalities can occur in any cell along ontogeny. Since somatic cells of eukaryotes are more likely to be in interphase, the solution of the problem concerning studying postmitotic cells and larger cell populations is interphase cytogenetics, which has become more or less applicable for specific biomedical tasks due to achievements in molecular cytogenetics (i.e. developments of fluorescence in situ hybridization -- FISH, and multicolor banding -- MCB). Numerous interphase molecular cytogenetic approaches are restricted to studying specific genomic loci (regions) being, however, useful for identification of chromosome abnormalities (aneuploidy, polyploidy, deletions, inversions, duplications, translocations). Moreover, these techniques are the unique possibility to establish biological role and patterns of nuclear genome organization at suprachromosomal level in a given cell. Here, it is to note that this issue is incompletely worked out due to technical limitations. Nonetheless, a number of state-of-the-art molecular cytogenetic techniques (i.e multicolor interphase FISH or interpahase chromosome-specific MCB) allow visualization of interphase chromosomes in their integrity at molecular resolutions. Thus, regardless numerous difficulties encountered during studying human interphase chromosomes, molecular cytogenetics does provide for high-resolution single-cell analysis of genome organization, structure and behavior at all stages of cell cycle.

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Fluorescence intensity profiles of in situ hybridization signals depict genome architecture within human interphase nuclei

Abstract: An approach towards construction of two dimensional (2D) and three dimensional (3D) profiles of interphase chro

Cited by 4 publications

References 13 publications

Interphase Quantitative Fluorescence in Situ Hybridization (IQ-FISH)

Interphase Quantitative Fluorescence in Situ Hybridization (IQ-FISH)

Yuri B. Yurov (1951-2017)

Human interphase chromosomes: a review of available molecular cytogenetic technologies

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