Fluorescence kinetics of Trp–Trp dipeptide and its derivatives in water via ultrafast fluorescence spectroscopy

Jia, Menghui; Hua, Yi; Chang, Mengfang; Cao, Xiaodan; Li, Lei; Zhou, Zhongneng; Pan, Haifeng; Chen, Yan; Zhang, Sanjun; Xu, Jianhua

doi:10.1016/j.jphotobiol.2015.06.014

Cited by 24 publications

(22 citation statements)

References 36 publications

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“…The details of the picosecond resolved TCSPC system and the femtosecond-resolved up-conversion system have been described elsewhere. 7,18,19…”

Section: Methodsmentioning

confidence: 99%

“…The details of the picosecond resolved TCSPC system and the femtosecond-resolved up-conversion system have been described elsewhere. 7,18,19 Results and Discussion Figure 1 shows the absorption and fluorescence spectra of DASPMIs in PBS buffer. The o-DASPMI and p-DASPMI dyes have absorption bands in the visible region with maxima at 425 nm and 450 nm, respectively.…”

Section: Optical Measurementsmentioning

confidence: 99%

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Using Pyridinium Styryl Dyes as the Standards of Time-Resolved Instrument Response

Chang

Hua

et al. 2016

Appl Spectrosc

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In this paper, two pyridinium styryl dyes, [2-(4-dimethylamino-phenyl)-vinyl]-1-methylpyridinium iodide (DASPMI), were synthesized and characterized by steady state fluorescence spectroscopy as well as picosecond and femtosecond time-resolved fluorescence spectroscopies. Both dyes exhibit large Stokes shifts and fluorescence decays equivalent to the instrument response function (IRF) standards employed in time-correlated single-photon counting. Due to their styryl and pyridinium moieties, DASPMIs have higher peak fluorescence intensity and shorter excited-state lifetimes than iodide ion-quenched fluorophores. The fluorescence lifetimes of o-DASPMI and p-DASPMI were measured to be 6.6 ps and 12.4 ps, respectively. The fluorescence transients of these DASPMIs were used as the IRFs for iterative reconvolution fitting of the time-resolved fluorescence decay profiles of Rhodamine B (RhB), sulforhodamine B (SRB), and the SRB-SRB2m RNA aptamer complex. The quality of the fits employing the DASPMI-derived IRFs are consistently equivalent to those employing IRFs obtained from light scattering. These results indicate that DASPMI-derived IRFs may be suited for a broad range of applications in time-resolved spectroscopy and fluorescence lifetime imaging microscopy (FLIM), especially in the visible emission range.

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“…The details of the picosecond resolved TCSPC system and the femtosecond-resolved up-conversion system have been described elsewhere. 7,18,19…”

Section: Methodsmentioning

confidence: 99%

Section: Optical Measurementsmentioning

confidence: 99%

Using Pyridinium Styryl Dyes as the Standards of Time-Resolved Instrument Response

Chang

Hua

et al. 2016

Appl Spectrosc

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“…The full width at half maximum (FWHM) of the IRF curve obtained from the scattering of SiO 2 particles was 190 ps for the TCSPC system. The details of this system have been described elsewhere [26,30].…”

Section: Optical Measurementsmentioning

confidence: 99%

“…It is insensitive to pH variation in the physiological pH range [24,25]. When Trp is connected to another amino acid (X), it is possible to tune the pH responding range of the Trp-X and X-Trp dipeptides to the physiological range due to the energy transfer between Trp and X [26][27][28][29]. However, using Trp-contained dipeptides as an intrinsic indicator for pH values was seldom reported.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence dynamics of N-terminal Trp–Trp residues in polypeptide: intrinsic indicators for monitoring pH

Hua

Chang

et al. 2015

Science Bulletin

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“…Fluorescence lifetime imaging and modeling have offered new solutions to many problems. For example, computational and mathematical modeling of fluorescence lifetime imaging can discriminate between NADH and NADPH 25 , and the fluorescent co-enzymes NADH and flavin adenine dinucleotide (FAD) 26 . Recently, R. Mongeon et al .…”

Section: Introductionmentioning

confidence: 99%

Using Fractional Intensities of Time-resolved Fluorescence to Sensitively Quantify NADH/NAD+ with Genetically Encoded Fluorescent Biosensors

Chang

et al. 2017

Sci Rep

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In this paper, we propose a novel and sensitive ratiometric analysis method that uses the fractional intensities of time-resolved fluorescence of genetically encoded fluorescent NADH/NAD+ biosensors, Peredox, SoNar, and Frex. When the conformations of the biosensors change upon NADH/NAD+ binding, the fractional intensities (α i τ i) have opposite changing trends. Their ratios could be exploited to quantify NADH/NAD+ levels with a larger dynamic range and higher resolution versus commonly used fluorescence intensity and lifetime methods. Moreover, only one excitation and one emission wavelength are required for this ratiometric measurement. This eliminates problems of traditional excitation-ratiometric and emission-ratiometric methods. This method could be used to simplify the design and achieve highly sensitive analyte quantification of genetically encoded fluorescent biosensors. Wide potential applications could be developed for imaging live cell metabolism based on this new method.

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Fluorescence kinetics of Trp–Trp dipeptide and its derivatives in water via ultrafast fluorescence spectroscopy

Cited by 24 publications

References 36 publications

Using Pyridinium Styryl Dyes as the Standards of Time-Resolved Instrument Response

Using Pyridinium Styryl Dyes as the Standards of Time-Resolved Instrument Response

Fluorescence dynamics of N-terminal Trp–Trp residues in polypeptide: intrinsic indicators for monitoring pH

Using Fractional Intensities of Time-resolved Fluorescence to Sensitively Quantify NADH/NAD+ with Genetically Encoded Fluorescent Biosensors

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