Fluorescence lifetime imaging microscopy (FLIM) of human middle ear tissue samples

Enzian, Paula; Lange, Birgit; Penxová, Zuzana; Leichtle, Anke; Miura, Yoko; Bruchhage, Karl-Ludwig; Brinkmann, Ralf

doi:10.1117/12.2670902

Cited by 1 publication

(2 citation statements)

References 18 publications

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“…Middle-ear tissue diagnosis Raman spectroscopy [7,8] Autofluorescence imaging [9][10][11] Middle-ear fluid detection and differentiation SWIR imaging [12] Raman spectroscopy [13] Tympanic membrane visualization OCT [14,15] SWIR imaging [12] In vivo…”

Section: Ex Vivomentioning

confidence: 99%

“…Enzian et al reported using fluorescence lifetime imaging microscopy (FLIM) on cryopreserved human middle-ear tissue samples and they were able to observe different fluorescence lifetimes in the 500-575 nm emission range with 473 nm excitation. This represents a promising and different technique, taking advantage of the autofluorescence properties of molecules such as collagen, elastin, and keratin present in the middle ear [11].…”

Section: Tissue Diagnosismentioning

confidence: 99%

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Label-Free Optical Technologies for Middle-Ear Diseases

Zhou,

Pandey,

Valdez

2024

Bioengineering

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Medical applications of optical technology have increased tremendously in recent decades. Label-free techniques have the unique advantage of investigating biological samples in vivo without introducing exogenous agents. This is especially beneficial for a rapid clinical translation as it reduces the need for toxicity studies and regulatory approval for exogenous labels. Emerging applications have utilized label-free optical technology for screening, diagnosis, and surgical guidance. Advancements in detection technology and rapid improvements in artificial intelligence have expedited the clinical implementation of some optical technologies. Among numerous biomedical application areas, middle-ear disease is a unique space where label-free technology has great potential. The middle ear has a unique anatomical location that can be accessed through a dark channel, the external auditory canal; it can be sampled through a tympanic membrane of approximately 100 microns in thickness. The tympanic membrane is the only membrane in the body that is surrounded by air on both sides, under normal conditions. Despite these favorable characteristics, current examination modalities for middle-ear space utilize century-old technology such as white-light otoscopy. This paper reviews existing label-free imaging technologies and their current progress in visualizing middle-ear diseases. We discuss potential opportunities, barriers, and practical considerations when transitioning label-free technology to clinical applications.

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