Fluorescence lifetime spectroscopy for guided therapy of brain tumors

Butte, Pramod; Mamelak, Adam N.; Nuño, Miriam A; Bannykh, Serguei; Black, Keith L.; Marcu, Laura

doi:10.1016/j.neuroimage.2010.11.001

Cited by 85 publications

(108 citation statements)

References 58 publications

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“…In 2011, they reported on the potential of applying TR-LIFS for intraoperative diagnosis of gliomas. 78 Normal cortex (N ¼ 35) and white matter measurements (N ¼ 12) showed longer-lasting fluorescence emission at 390 nm compared with 460 nm, whereas the 390-nm emission peak was absent in lowgrade gliomas and reduced in high-grade gliomas. The system required a relatively long data acquisition time (∼30 s) to capture each tissue measurement, which would need to be shortened substantially for effective integration in clinical workflows.…”

Section: Time-domain Fluorescence Measurement Methodsmentioning

confidence: 97%

“…It could be a more effective tool for optical biopsy because not only does the contrast from different fluorescence lifetimes result from changes of cellular or tissue environment, but also these changes are independent of fluorescent signal intensity, irregular brain surfaces, tissue illumination, blood, and fluorophore quantum yield. 61,66 Time-resolved fluorescence measurement can also differentiate the fluorescence components of overlapping emission spectra, providing the ability to detect variations in pH and temperature. 59,67 However, time-resolved fluorescence measurements (either spectroscopic or imaging) require more complex equipment, including more sophisticated lasers, high-speed detectors, and electronics that function in the picosecond range.…”

Section: Time-domain Fluorescence Measurement Methodsmentioning

confidence: 99%

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Review of the potential of optical technologies for cancer diagnosis in neurosurgery: a step toward intraoperative neurophotonics

Vasefi

MacKinnon²,

Farkas

et al. 2016

Neurophoton

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Section: Time-domain Fluorescence Measurement Methodsmentioning

confidence: 97%

Section: Time-domain Fluorescence Measurement Methodsmentioning

confidence: 99%

Review of the potential of optical technologies for cancer diagnosis in neurosurgery: a step toward intraoperative neurophotonics

Vasefi

MacKinnon²,

Farkas

et al. 2016

Neurophoton

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“…The first study has shown FLIM applicability for evaluating resection borders during neurosurgical intervention on brain tumor in vivo [96]. FlIM spectroscopy has been used in patients with head and neck tumors to reveal that tumor tissue cells have shorter NADH fluorescence lifetime as compared to the surrounding healthy tissue [108][109][110], which suggests their glycolytic status.…”

Section: Nadh and Fad Fluorescence Lifetime In Energy Metabolism Evalmentioning

confidence: 99%

Metabolic Imaging in the Study of Oncological Processes (Review)

Lukina¹,

Shirmanova²,

Sergeeva³

et al. 2016

Sovrem Tehnol Med

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There have been reviewed the main approaches to the study of energy metabolism in cancer cells, based on fluorescent imaging of NADH and FAD cofactors. The term "metabolic imaging" covers a range of modern fluorescent techniques for detecting NADH and FAD according to fluorescence intensity and/or lifetime. These cofactors play an important role in energy metabolism reactions acting as electron carriers and, being fluorescent, serve as a basis for metabolic process analysis in living cells and tissues without the use of additional coloring agents. Particular attention is paid to metabolic changes associated with carcinogenesis. Numerous examples of metabolic imaging application in cell cultures in vitro, animal and human tumors in vivo, as well as in patients' tumor biopsy samples have demonstrated its being highly demanded for biomedical research in the area of oncology.Key words: metabolic imaging; energy metabolism; fluorescence lifetime; redox ratio; NADH; FAD; oxidative phosphorylation; glycolysis; tumor cells.For contacts: Mariya M. lukina, e-mail: kuznetsova.m.m@yandex.ru Cells need energy to maintain homeostasis. All processes of cell activity, such as generating concentration gradients, cytoskeleton movement, DNA repair, transcription, translation and vesicular transport, are energy-dependent. Energy metabolism of normal cells is known to differ significantly from cell metabolism in case of a disease. Therefore, the metabolic status can serve as an indicator for diagnosis and visualization of response to pathological process treatment. Taking into account high incidence of oncological diseases, assessment of metabolic phenotype of tumor cells is particularly urgent.Development of optical visualization technologies has provided the possibility of noninvasive analysis of metabolic cofactors NADH and FAD in living tumor cells at high spatial resolution (up to several hundred Metabolic Imaging in the Study of Oncological Process nanometers) without using additional coloring agents and with no significant effect on biochemical and physiological condition of cells. The term "metabolic imaging" covers a range of modern fluorescent techniques which allow visualizing NADH and FAD according to their fluorescence intensity and/or lifetime.The present review characterizes the properties of energy metabolism in cancer cells, describes the two key approaches to the assessment of metabolic status: analysis of cofactor fluorescence intensity ratio (redox ratio) and their fluorescence lifetime. There have been presented numerous examples of metabolic imaging application in cell cultures in vitro, animal and human tumors in vivo, as well as in patients' tumor biopsy samples.

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“…An endoscopic procedure for which 5-ALA is administered is well established as a diagnostic tool for cancers such as bladder, oral, laryngeal, esophageal, bronchial tract, and peritoneal cancer [42][43][44][45].…”

Section: Neuroendoscopy With 5-alamentioning

confidence: 99%

Review of Neurosurgical Fluorescence Imaging Systems for Clinical Application

Kim

Lee

Yoo

et al. 2016

Journal of the Optical Society of Korea

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A number of fluorescence imaging techniques for use in the surgical removal of glioma have been developed over the course of the long history of neurosurgery. Various biomarkers, biochemical agents, and detection systems for glioma have also been developed. This review focuses on 5-aminolevulinic acid (5-ALA), which is used to detect glioma. Numerous forms of fluorescence-guided surgery use 5-ALA, which is helpful to the surgeon. The surgical microscope system is the observational method generally used with 5-ALA, while the loupe, endoscope, and exoscope are simpler alternatives. A system is needed for minimal resection and other issues that arise during neurosurgery. Such an enhanced system should be able to detect low-grade tumors and provide information on microinvasive diseases, resulting in an improved survival rate and better surgical skills. Development of systems that fulfill certain needs would help protect the brain function of the patient and broaden the use of such systems in neurosurgery.

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Fluorescence lifetime spectroscopy for guided therapy of brain tumors

Cited by 85 publications

References 58 publications

Review of the potential of optical technologies for cancer diagnosis in neurosurgery: a step toward intraoperative neurophotonics

Review of the potential of optical technologies for cancer diagnosis in neurosurgery: a step toward intraoperative neurophotonics

Metabolic Imaging in the Study of Oncological Processes (Review)

Review of Neurosurgical Fluorescence Imaging Systems for Clinical Application

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