Fluorescence method for kanamycin detection based on the conversion of G-triplex and G-quadruplex

Wang, Chengke; Li, Jiang-Yu

doi:10.1007/s00216-021-03676-y

Cited by 13 publications

(5 citation statements)

References 24 publications

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“…As shown in Figure B, the surface of ZIF-67-Fe collapsed and wrinkled to form a cage-like structure of Fe–Co/NPC after 2 h of pyrolysis in N 2 at 500 °C. The collapsed rough surface of the Fe–Co/NPC can adsorb more aptamers, and the addition of Fe atoms enhanced the electrical signal by improving the electron-transfer efficiency . In Figure C, XPS image of Fe–Co/NPC showed a peak near 720 eV, indicating the presence of Fe atoms.…”

Section: Resultsmentioning

confidence: 99%

“…This indicates that the embedding of OPs into the aptamer leads to a reduction of DNA base-stacking interactions. Meanwhile, after the binding of the OPs to the G-base-rich aptamer, the peaks shifted flat to the left due to the conversion of the unstructured conformation to a parallel conformation, indicating the successful formation of a parallel G-triplex structural mixture …”

Section: Resultsmentioning

confidence: 99%

“…The collapsed rough surface of the Fe−Co/NPC can adsorb more aptamers, and the addition of Fe atoms enhanced the electrical signal by improving the electron-transfer efficiency. 45 In Figure 6C, XPS image of Fe−Co/NPC showed a peak near 720 eV, indicating the presence of Fe atoms. To demonstrate the feasibility of the method, the electrochemical properties of the assembled electrodes were examined using EIS, CV, and DPV, respectively.…”

Section: Characterization and Electrochemical Detectionmentioning

confidence: 97%

“…Meanwhile, after the binding of the OPs to the G-base-rich aptamer, the peaks shifted flat to the left due to the conversion of the unstructured conformation to a parallel conformation, indicating the successful formation of a parallel G-triplex structural mixture. 45 To study the binding mechanism between the aptamers and OPs, molecular docking models of OPs 0−12 with four OPs (DCH, DCP, PHT, and DIC) were constructed. Figure 3D and Figure S7 depict the three-dimensional structure of the aptamer OPs 0−12 bound to each of the four OPs.…”

Section: Sequence Analysis Of Selectedmentioning

confidence: 99%

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Novel Broad-Spectrum Aptamer Used to Build Fe–Co Nanoporous Carbon Highly Sensitive Sensing Platform for the Detection of Multiple Organophosphorus Pesticides

Wang

Liu

Shen

et al. 2023

J. Agric. Food Chem.

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In this work, broad-spectrum aptamers for organophosphorus pesticides (OPs) were obtained by alternate target systematic evolution of ligands by exponential enrichment screening. The secondary and tertiary structure analyses of the aptamer inferred that the neck-loop structure formed a G-triplex structure with the target. In addition, optimization of the sheared aptamer resulted in a stronger affinity (K d = 86.74 nM), which was increased by 2 orders of magnitude compared to similar aptamers. A novel electrochemical biosensor was prepared by modifying an aptamer labeled with an electroactive substance (methylene blue) on the surface of nanoporous carbon containing Fe–Co (Fe–Co/NPC). When a target bound to the aptamer, a G-triplex structure was formed close to the electrode surface. The aptamer phosphate backbone labeled with methylene blue enhanced the electron-transfer efficiency and resulted in signal changes. The biosensor exhibited an excellent sensitivity (7.32 fM) and a wide detection range (1 × 10–13 to 1 × 10–3 M) for OPs under optimal conditions, enabling simultaneous detection of multiple OPs in vegetables.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Characterization and Electrochemical Detectionmentioning

confidence: 97%

Section: Sequence Analysis Of Selectedmentioning

confidence: 99%

See 2 more Smart Citations

Novel Broad-Spectrum Aptamer Used to Build Fe–Co Nanoporous Carbon Highly Sensitive Sensing Platform for the Detection of Multiple Organophosphorus Pesticides

Wang

Liu

Shen

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

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“…However, most studies have primarily focused on the effects of pH, DNA self-modification, and ligands on the i-motif structure, utilizing techniques such as circular dichroism (CD), , nuclear magnetic resonance (NMR), ultraviolet–visible spectroscopy (UV–vis), fluorescence resonance energy transfer (FRET), and so on. While these methods have been successful in characterizing the i-motif structure, they also present certain challenges.…”

Section: Introductionmentioning

confidence: 99%

Nanopore-Based Single-Molecule Investigation of Cation Effect on the i-Motif Structure

Wang,

Cui,

Liu

et al. 2024

J. Phys. Chem. B

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The i-motif, a secondary structure of a four-helix formed by cytosine-rich DNA (i-DNA) through C−C + base pairing, is prevalent in human telomeres and promoters. This structure creates steric hindrance, thereby inhibiting both gene expression and protein coding. The conformation of i-DNA is intricately linked to the intracellular ionic environment. Hence, investigating its conformation under various ion conditions holds significant importance. In this study, we explored the impact of cations on the i-motif structure at the single-molecule level using the α-hemolysin (α-HL) nanochannel. Our findings reveal that the ability of i-DNA to fold into the i-motif structure follows the order Cs + > Na + > K + > Li + for monovalent cations. Furthermore, we observed the interconversion of single-stranded DNA (ss-DNA) and the i-motif structure at high and low concentrations of Mg 2+ and Ba 2+ electrolyte solutions. This study not only has the potential to extend the application of i-motifbased sensors in complex solution environments but also provides a new idea for the detection of metal ions.

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A label-free T4 polynucleotide kinase fluorescence sensor based on split dimeric G-quadruplex and ligation-induced dimeric G-quadruplex/thioflavin T conformation

et al. 2022

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Fluorescence method for kanamycin detection based on the conversion of G-triplex and G-quadruplex

Cited by 13 publications

References 24 publications

Novel Broad-Spectrum Aptamer Used to Build Fe–Co Nanoporous Carbon Highly Sensitive Sensing Platform for the Detection of Multiple Organophosphorus Pesticides

Novel Broad-Spectrum Aptamer Used to Build Fe–Co Nanoporous Carbon Highly Sensitive Sensing Platform for the Detection of Multiple Organophosphorus Pesticides

Nanopore-Based Single-Molecule Investigation of Cation Effect on the i-Motif Structure

A label-free T4 polynucleotide kinase fluorescence sensor based on split dimeric G-quadruplex and ligation-induced dimeric G-quadruplex/thioflavin T conformation

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