Fluorescence microscope by using computational ghost imaging

Abstract

Abstract. We propose a fluorescence microscope by using the computational Ghost imaging (CGI) for observing a living cell for a long duration over an hour. There is a problem for observing a cell about light-induced bleaching for a ling-term observation. To over come the problem, we focused on an advantage of sensitivity of the CGI as second order colleration for an imaging with weak intensity excitation light. Setting for the CGI, a DMD projector was installed at an eye-piece part of a microscope and fluorescent light was detected using a bucket detector of a photo-multiplier tube. As a results, we have shown the imaging advantage of the CGI under weak light intensity, in addition, we have demonstrated to detect fluorescence images of biological samples for one day.

BM3D-based color computational ghost imaging

“…Therefore, in the process of the gradual application of classical light sources to the field of ghost imaging, new concepts, new methods * Author to whom any correspondence should be addressed. and new technologies of ghost imaging have been vigorously developed [19][20][21][22][23][24][25][26][27][28][29][30][31]. However, the above methods and technologies focused on a single wavelength light source, so that the reconstructed images are only gray scale images, which limits its application field.…”