Fluorescence Microscopy Applied to Entomology and Allied Fields

Metcalf, Robert L.; Patton, R. L.

doi:10.3109/10520294409105821

Cited by 29 publications

(12 citation statements)

References 9 publications

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“…The fixative used was ordinary corrosive formol. Metcalf and Patton (1944) state that the salts of heavy metals should be avoided, but this finding has not been confirmed in this investigation. The sections were treated with iodine and hypo in the usual way.…”

Section: Present Investigationcontrasting

confidence: 64%

“…Opinions differ concerning fluorite and apochromatic lenses. Graham (1943) and Matthaei (1950) find them unsatisfactory, but Metcalf and Patton (1944) obtained good results, as did Hagemann in his original work. The use of an immersion objective has been mentioned (Kolmer, Spaulding, and Robinson, 1951), but in general there is no need for any power higher than 4 mm.…”

Section: Apparatusmentioning

confidence: 86%

“…Graham (1942) recommended a 500 w. projection lamp, though others preferred the mercury vapour lamp (Kloeck and Sweany, 1943;Stevens, 1945). Of the latter type, however, the 125 w. commercial " black lamp" did not give full field illumination with low-power objectives (Lempert, 1944;Norman and Jelks, 1945). Spectrographic investigations by Graham (1943) and Hughes (1943) showed that the effective wavelengths for the fluorescence of auramine extend well above the ultra-violet, and that the mercury vapour lamp is not an essential.…”

Section: Apparatusmentioning

confidence: 99%

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The Development of Fluorescence Microscopy for Tubercle Bacilli and its Use as an Adjunct to Histological Routine

McClure¹

1953

Journal of Clinical Pathology

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Section: Present Investigationcontrasting

confidence: 64%

Section: Apparatusmentioning

confidence: 86%

Section: Apparatusmentioning

confidence: 99%

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The Development of Fluorescence Microscopy for Tubercle Bacilli and its Use as an Adjunct to Histological Routine

McClure¹

1953

Journal of Clinical Pathology

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“…Sections were stained with Sudan black B in ethylene glycol (Jensen 1962). Rose Bengal (0.01%, w/v, aqueous solution) was used as a fluorochrome for fats (Metcalf and Patton 1944). Fresh sections were also used to observe autofluorescence and auramine 0 staining.…”

Section: Methodsmentioning

confidence: 99%

Preliminary evidence for the involvement of suberization in infection of Casuarina

Berg¹

1983

Can. J. Bot.

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Histochemistry of infected cells in mature nodule lobes of Casuarina showed that walls of infected host cells had a ligninlike component (ultraviolet-stimulated autofluorescence and staining with auramine O, phloroglucinol staining, and resistance to degradation by hydrolytic enzymes). Cytoplasm of infected cells had a pronounced affinity for lipid stains (Sudan black B, Rose Bengal fluorescence), though walls of infected cells were less clearly stained. When nodules were digested several days in cold 50% chromic acid, the walls of infected cells and suberized host tissue (epidermis, endodermis) were not degraded. Endophyte cell wall components were also found to be resistant to chromic acid digestion. The digested tissue retained the capacity to adsorb lipid dyes. These observations suggested that walls of infected host cells had become impregnated with a suberinlike compound. The hydrophobic quality of this wall was evident when its ultrastructure was examined after en bloc staining with the polar stain KMnO4. This stain did not penetrate the walls of mature infected cells, perhaps because of the presence of aliphatic compounds similar to those found in suberin. As is known for suberizing tissue, peroxidase activity (via diaminobenzidine oxidation) was high in nodule cortical tissue cell walls. The peroxidase stain was also localized on endophyte hyphae. This report is the first instance associating a suberizationlike host reaction with infection of an actinorhizal plant.

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“…Thereafter a few papers concerning this field have been published (4,5,6). In my laboratory, a method combining the lumiflavin fluorescence with the riboflavin fluorescence has been devised (7,8,9,10).…”

mentioning

confidence: 99%