Fluorescence Microscopy for Algal Studies

Wilde, E.W.; Fliermans, C.B.

doi:10.2307/3225943

Cited by 24 publications

(15 citation statements)

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“…The use of epifluorescence microscopy is needed to assess the truly photosynthetic fraction of the plankton. The use of such methodology, although not a novelty (Wilde & Fliermans, 1979;Davis & Sieburth, 1982), can only provide reliable data when operational circumstances allow for the appropriate storage and quick analysis of samples to counteract fairly rapid loss of chlorophyll fluorescence (and/or the fluorescence of other accessory pigments or stains of interest).…”

Section: Introductionmentioning

confidence: 99%

Auto- and heterotrophic nanoplankton and filamentous bacteria of Guanabara Bay (RJ, Brazil): estimates of cell/filament numbers versus carbon content

et al. 2007

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Variations of nanoplankton (2-20 µm) and filamentous bacteria (diameter: 0.5-2.0 µm) of Guanabara Bay (RJ, Brazil) are presented, considering cell density and carbon content of auto- and heterotrophs. Our goal is to contribute to future modeling of local trophic dynamics. Subsurface water samples were taken weekly during the year 2000 at two sites: Urca (close to the entrance, more saline, eutrophic) and Ramos (inner area, less saline, hypertrophic). Microscopic analysis was done by epifluorescence and cell density was converted to biomass through cell biovolume. Total nanoplankton was about 10(8) cells.l-1 in most samples (>57%), and total filamentous bacteria densities varied from 10(5) to 10(8) fil.l-1. Autotroph density was one order of magnitude higher at Ramos, both for nanoplankton (Md: 10(8)cells.l-1 at Ramos and 10(7)cells.l-1 at Urca) and for filamentous bacteria (Md: 10(6) fil.l-1 at Ramos and 10(5) fil.l-1 at Urca). The same was observed for autotrophic biomass (Md: 10³µgC.l-1 at Ramos and 10¹µgC.l-1 at Urca for nanoplankton; Md: 28µgC.l-1 at Ramos and 1.4µgC.l-1 at Urca for filamentous bacteria). The relative contribution of autotrophs increased after conversion to biomass. Seasonal variation was conspicuous for filamentous bacteria at both sites and for nanoplankton only at Ramos, with maximum autotrophic abundances during the rainy period (spring-summer).
Variações do nanoplâncton (2-20µm) e bactérias filamentosas (diâmetro: 0.5-2.0 µm) da Baía de Guanabara (RJ, Brasil) são apresentadas, considerando densidade celular e biomassa de autótrofos e heterótrofos. A meta deste trabalho é contribuir para uma futura modelagem da dinâmica trófica neste sistema. Amostras subsuperficiais de água foram coletadas semanalmente durante um ano em dois pontos: Urca (próximo à entrada, mais salino, eutrófico) e Ramos (no interior, menos salino, hipertrófico). Foi feita análise por microscopia de epifluorescência, com densidade celular convertida para biomassa através do biovolume celular. A concentração do nanoplâncton total foi alta (10(8)cel.l-1) na maioria das amostras (>57%) e das bactérias filamentosas variou de 10(5) a 10(8)fil.l-1. A densidade de autótrofos em Ramos foi uma ordem de grandeza superior tanto para o nanoplâncton (Md: 10(8)cel.l-1 em Ramos e 10(7)cel.l-1 na Urca) quanto para as bactérias filamentosas (Md: 10(6)fil.l-1 em Ramos e 10(5)fil.l-1 na Urca). A biomassa autotrófica do nanoplâncton (Md: 10³µgC.l-1 em Ramos e 10¹µgC.l-1 na Urca) e das bactérias filamentosas (Md: 28µgC.l-1 em Ramos e 1,4µgC.l-1 na Urca) seguiu o mesmo padrão. A contribuição relativa de autótrofos aumentou após a conversão para biomassa. Uma tendência temporal foi evidenciada para as bactérias filamentosas em ambos os pontos e, para o nanoplâncton autotrófico, em Ramos, com valores máximos no período chuvoso (primavera-verão)

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Section: Introductionmentioning

confidence: 99%

Auto- and heterotrophic nanoplankton and filamentous bacteria of Guanabara Bay (RJ, Brazil): estimates of cell/filament numbers versus carbon content

et al. 2007

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“…A 2.2 ml aliquot was then placed in a counting chamber, allowed to settle for at least two hours, and examined at 30ooX, 75oX, and ii 8oX using a Zeiss Invertoscope equipped with fluorescence microscopical capabilities. Taxonomic advantages of using fluorescence illumination in combination with a conventional light source have been discussed by Wilde & Fliermans (1979). The principal disadvantage of this microscopical technique was that some diatoms could not be positively identified to species and had to be lumped at the genus level.…”

Section: Methodsmentioning

confidence: 99%

Structural characteristics of algal communities in thermally altered artificial streams

Wilde

Tilly

1981

Hydrobiologia

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Algal community structure on natural substrates of thermally altered artificial streams was studied for one year. The streams were fed by a natural blackwater stream near Aiken, South Carolina. Temperature-related shifts in the abundance of major species were demonstrated. Red algae were absent from a stream heated 12.5°C above ambient, but remained abundant in streams heated 7.5 0 C or less. Water temperatures above 30°C produced blue-green algal dominance and eliminated several indigenous species. Substrate specificity was exhibited by all major taxa. Several taxa were abundant only on the bottom sediments and some showed a distinct affinity for either sand or silt, the principal bottom types.

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“…Lugol's solution may also be used, but glutaraldehyde preserves chlorophyll autofluorescence. Wilde and Fliermans ( 1979) reported retention of chlorophyll autofluorescence in glutaraldehyde-preserved samples after storage for 9 months at 9°C. However, samples should be processed within a few days to ensure against loss of fluorescence intensity in preserved samples (cf.…”

Section: Limnolmentioning

confidence: 99%

A simple and reliable method for making permanent mounts of phytoplankton for light and fluorescence microscopy1

Crumpton

1987

Limnology & Oceanography

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A simple and reliable method is described for making permanent mounts of phytoplankton for critical microscopy and cell counting. Phytoplankton are fixed with glutaraldehyde, concentrated by gentle vacuum onto cellulosic membrane filters, and mounted directly in a water‐soluble, methacrylic resin. The resulting mounts are permanent and suitable for even very delicate nanoflagellates. Chlorophyll autofluorescence is preserved as is the structural integrity of cells. Resolution is very good and mounts can be examined with bright‐field, phase contrast, differential interference contrast, and fluorescence microscopy.

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Fluorescence Microscopy for Algal Studies

Cited by 24 publications

References 0 publications

Auto- and heterotrophic nanoplankton and filamentous bacteria of Guanabara Bay (RJ, Brazil): estimates of cell/filament numbers versus carbon content

Auto- and heterotrophic nanoplankton and filamentous bacteria of Guanabara Bay (RJ, Brazil): estimates of cell/filament numbers versus carbon content

Structural characteristics of algal communities in thermally altered artificial streams

A simple and reliable method for making permanent mounts of phytoplankton for light and fluorescence microscopy1

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