Fluorescence monitoring of a benzo[a]pyrene metabolite using a regenerable immunochemical-based fiber-optic sensor

Alarie, Jean Pierre; Bowyer, James R.; Sepaniak, Michael J.; Hoyt, A.M.; Vo‐Dinh, Tuan

doi:10.1016/s0003-2670(00)83317-6

Cited by 47 publications

(24 citation statements)

References 12 publications

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“…Over 95% of the antibody activity has been shown to be retained when using this procedure. (19) When storage over long periods of time (greater than 5 days) was required, the fibers were placed in PBS and stored at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Intracellular Measurements in Mammary Carcinoma Cells Using Fiber-Optic Nanosensors

Cullum

Griffin

Miller

et al. 2000

Analytical Biochemistry

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109

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Section: Methodsmentioning

confidence: 99%

Intracellular Measurements in Mammary Carcinoma Cells Using Fiber-Optic Nanosensors

Cullum

Griffin

Miller

et al. 2000

Analytical Biochemistry

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109

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“…Amperometric biosensors for naphthalene found in contaminated soils, were constructed using Sphingomonas yanoikuyae B1 [78]. For benzo(a)pyrene (BaP) and related adducts, a fiber optic fluoroimmunosensor has also been developed in which high sensitivity is achieved by laser excitation and optical detection [79]. The laser radiation reaches the sensor probe and excites the BaP bound to the fiber optic probe.…”

Section: Alkanes Aromatic Compounds and Polycyclic Aromatic Hydrocamentioning

confidence: 99%

Biosensors for environmental applications: Future development trends

Rodríguez-Mozaz

Marco

Alda

et al. 2004

Pure and Applied Chemistry

199

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Biosensors can be excellent analytical tools for monitoring programs working to implement legislation. In this article, biosensors for environmental analysis and monitoring are extensively reviewed. Examples of biosensors for the most important families of environmental pollutants, including some commercial devices, are presented. Finally, future trends in biosensor development are discussed. In this context, bioelectronics, nanotechnology, miniaturization, and especially biotechnology seem to be growing areas that will have a marked influence on the development of new biosensing strategies in the next future.

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“…69 Examples of immunosensors which utilize the intrinsic fluorescence of the analyte are not widespread; however, they are used in the specific area of PAH detection. As an example, Alaire et al 70 described a fiberoptic immunosensor for benzo[a]pyrene which relied on the direct detection of the bound analyte using evanescent excitation. In this case, a LOD of 20 nM and a dynamic range of spanning three orders of magnitude could be obtained with an equilibration time of 20 min, although a washing step was required to remove unbound analyte.…”

Section: Fluoroimmunosensorsmentioning

confidence: 99%

Fluorescence‐Based Biosensors

Brennan

2000

Encyclopedia of Analytical Chemistry

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Biosensors, as defined by Biosensors and Bioelectronics, are analytical devices “incorporating a biological material, a biologically derived material or biomimetic intimately associated with or integrated within a physicochemical transducer or transducing microsystem”. Fluorescence‐based biosensors are those devices that derive an analytical signal from the fluorescence emission process. Such biosensors may be used for a wide variety of tasks, including: detection of compounds of biomedical, 1 environmental 2 or defense interest, 3 on‐line monitoring for process control, 4 monitoring of foodstuffs, 5 selective detection of compounds undergoing a chemical separation, 6 and screening of drug compounds. 7 Advantages of such devices include: 8 high selectivity; rapid response times; reusability; amenability to remote analysis; and immunity from electrical interferences. The selective nature of the complexation between the biomolecule and the analyte, combined with the small size of the device and the advantages of total internal reflection (TIR)‐based spectroscopy, 9 also results in an ability to measure analytes in complex matrixes. Such samples may include highly scattering systems such as milk or whole blood, 10 or relatively inaccessible locations such as groundwater wells, or even intracellular environments. 11 The key limitation of such devices mainly centers around the poor stability of biological compounds, which can lead to a substantial drift in instrument response over time. There is also the potential for interferences related to biological autofluorescence, and the analyte‐dependent sensitivity and limit of detection (LOD) for sensors, which rely on the nature of both the protein and the fluorescent probe utilized. Finally, in cases where immunological reagents are used, the devices can show a lack of reversibility, and may operate only as a “one‐shot” screen, without the potential for continuous, quantitative analysis.

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Fluorescence monitoring of a benzo[a]pyrene metabolite using a regenerable immunochemical-based fiber-optic sensor

Cited by 47 publications

References 12 publications

Intracellular Measurements in Mammary Carcinoma Cells Using Fiber-Optic Nanosensors

Intracellular Measurements in Mammary Carcinoma Cells Using Fiber-Optic Nanosensors

Biosensors for environmental applications: Future development trends

Fluorescence‐Based Biosensors

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