Fluorescence of Muscle and Connective Tissue from Cod and Salmon

Abstract

Autofluorescence of salmon and cod muscle was measured and compared with autofluorescence of collagen type I and type V. Similarities between fluorescence of fish muscle and collagen were found in that the same peaks were obtained around 390, 430, and 480 nm. These similarities are supported by principal component analyses. Texture and gaping score were predicted from the fluorescence spectra by partial least-squares regression. However, the predictions did not perform well. Relating fluorescence to the gaping score gave a prediction error of 0.91 and a correlation of 0.43 when measuring gaping on a scale from 0 to 5. There was no relation between texture and fluorescence spectra. Fluorescence of fish muscle could be related to the storage time. However, this relation seemed not to be induced by changes in collagen.

Practical aspects of PARAFAC modeling of fluorescence excitation‐emission data

“…These relations could not be seen when comparing visually the intensities of the two peaks with the chill storage time. The change in fluorescence intensity with storage time has been shown in other studies [30,31]. However, the present study illustrates an explorative way of identifying chemically meaningful quality parameters that can possibly be monitored industrially.…”

Fluorescence in aldehyde model systems related to lipid oxidation

“…Model systems 3-5: Fluorescence induced by adding aldehydes to minced turkey, pork, and cod meat Fresh cod was obtained from a local commercial fishing vessel in the Oslofjord, Drøbak, Norway, and kept on ice for 3 days before processing. This pre-storing was done to avoid the fluorescence from NADH activity in the cod the first days after slaughtering (Andersen & Wold, 2003). Head and bones were kept on during storage.…”

Fluorescence Intrinsic Characterization of Excitation-Emission Matrix Using Multi-Dimensional Ensemble Empirical Mode Decomposition

“…The first five PCs were selected as the main components. We also compared the feature locations with the references listed in Table 1[1,4,5,13,15,16] and found that PCs with larger b values present features related to chemicals. The main components are PC 14 , PC 15 , PC 12 , PC 11 , and PC 8 in order of descending b values.…”

“…To control biological variation, fresh fish fillets were used as testing samples. Many important fluorophores, such as collagen type I, type V, and NADH, are related to the changes in fish during different stages [13]. The aim of this paper was to extract the intrinsic fluorescence characteristics that are related to the chemical constituents of tissue from EEM by using MEEMD and to compare its feasibility with the results from PCA.…”