Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections

Wegner, Kyle A; Keikhosravi, Adib; Eliceiri, Kevin W.; Vezina, Chad M.

doi:10.1369/0022155417718541

Cited by 94 publications

(88 citation statements)

References 45 publications

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“…Tissue was paraffin-embedded, sectioned, and stained with hematoxylin and eosin (H&E) or Masson's trichrome by the Experimental Animal Pathology Laboratory (Carbone Cancer Center, University of Wisconsin-Madison; Madison, WI, USA). Picrosirius red staining was completed as described [47]. Immunofluorescence was performed as described [103].…”

Section: Histology and Immunofluorescencementioning

confidence: 99%

Stromal CCL2 Signaling Promotes Mammary Tumor Fibrosis through Recruitment of Myeloid-Lineage Cells

Kuziel

Thompson

D'Amato

et al. 2020

Cancers

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Obesity is correlated with breast tumor desmoplasia, leading to diminished chemotherapy response and disease-free survival. Obesity causes chronic, macrophage-driven inflammation within breast tissue, initiated by chemokine ligand 2 (CCL2) signaling from adipose stromal cells. To understand how CCL2-induced inflammation alters breast tumor pathology, we transplanted oncogenically transformed human breast epithelial cells with breast stromal cells expressing CCL2 or empty vector into murine mammary glands and examined tumor formation and progression with time. As tumors developed, macrophages were rapidly recruited, followed by the emergence of cancer-associated fibroblasts (CAFs) and collagen deposition. Depletion of CD11b + myeloid lineage cells early in tumor formation reduced tumor growth, CAF numbers, and collagen deposition. CCL2 expression within developing tumors also enhanced recruitment of myeloid progenitor cells from the bone marrow into the tumor site. The myeloid progenitor cell population contained elevated numbers of fibrocytes, which exhibited platelet-derived growth factor receptor-alpha (PDGFRα)-dependent colony formation and growth in vitro. Together, these results suggest that chronic inflammation induced by CCL2 significantly enhances tumor growth and promotes the formation of a desmoplastic stroma through early recruitment of macrophages and fibrocytes into the tumor microenvironment. Fibrocytes may be a novel target in the tumor microenvironment to reduce tumor fibrosis and enhance treatment responses for obese breast cancer patients.

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Section: Histology and Immunofluorescencementioning

confidence: 99%

Stromal CCL2 Signaling Promotes Mammary Tumor Fibrosis through Recruitment of Myeloid-Lineage Cells

Kuziel

Thompson

D'Amato

et al. 2020

Cancers

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“…In addition to polarized light, picrosirius red staining can also be visualized with fluorescence microscopy, which is more sensitive, but does not show differences in fiber thicknesses (Wegner et al, ).…”

Section: Techniques To Analyze Collagenmentioning

confidence: 99%

Collagen analysis with mass spectrometry

Huizen

IJzermans

Burgers

et al. 2019

Mass Spectrometry Reviews

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Mass spectrometry‐based techniques can be applied to investigate collagen with respect to identification, quantification, supramolecular organization, and various post‐translational modifications. The continuous interest in collagen research has led to a shift from techniques to analyze the physical characteristics of collagen to methods to study collagen abundance and modifications. In this review, we illustrate the potential of mass spectrometry for in‐depth analyses of collagen.

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“…Wegner et al, [27] performed a study with fluorescencent PSR in prostate of C57BL/6J mice. They found that fluorescent PSR imaging was more sensitive than polarized light for identify the collagen fibers.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Collagens Fibers (I, III, IV) and Elastin in the Extracellular Matrix of Normal and Neoplastic Canine Prostate

Calderón¹,

Kobayashi²,

Vasconcelos³

et al. 2019

Preprint

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Collagen (Coll) is the most common protein in the extracellular matrix, responsible for providing tissue structure and support. In some types of cancer, including prostate cancer (PC) abundant collagen was identified and related to tumor progression and metastasis. This study aimed to investigate Coll-I, III, IV and elastin in normal canine prostatic tissue and PC, using the Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Col-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle and 5% around in blood vessels, in both normal prostate and PC. Immunostaining for type IV collagen was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and never fibers of normal and PC samples. Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples. A high amount of elastic fibers was observed around the ducts and the urethra in normal and canine PC. The distribution and area percentage of staining for collagen are similar in normal and neoplastic canine prostate when analyzed with PSR and IHC.

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Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections

Cited by 94 publications

References 45 publications

Stromal CCL2 Signaling Promotes Mammary Tumor Fibrosis through Recruitment of Myeloid-Lineage Cells

Stromal CCL2 Signaling Promotes Mammary Tumor Fibrosis through Recruitment of Myeloid-Lineage Cells

Collagen analysis with mass spectrometry

Characterization of Collagens Fibers (I, III, IV) and Elastin in the Extracellular Matrix of Normal and Neoplastic Canine Prostate

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