Fluorescence Polarization Imaging of Methylene Blue Facilitates Quantitative Detection of Thyroid Cancer in Single Cells

Jermain, Peter R.; Fischer, Andrew H.; Joseph, Lija; Muzikansky, Alona; Yaroslavsky, Anna N.

doi:10.3390/cancers14051339

Cited by 8 publications

(13 citation statements)

References 44 publications

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“…Both cationic dyes (MB and CV) are known to be exogenous fluorophores [41,42]. Therefore, their adsorption onto the surface of the biosorbents (WS H2O and WS NaOH , respectively) was analyzed by polarized light microscopy, as shown in Figure 5b-f.…”

Section: Adsorption Isothermsmentioning

confidence: 99%

An Eco-Friendly Modification of a Walnut Shell Biosorbent for Increased Efficiency in Wastewater Treatment

Enache¹,

Samoilă²,

Cojocaru³

et al. 2023

Sustainability

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Herein, we report the performance of some low-cost biosorbents developed by environment-friendly modification of walnut shells. Two types of biosorbents were prepared by ecological modification of walnut shell surfaces: (1) biosorbents obtained by hot water treatment (WSH2O) and (2) biosorbents produced by mercerization (WSNaOH). Different techniques were used to evaluate the morphological, elemental, and structural modification of the biosorbents, by comparison with raw materials. These characterization techniques involved scanning electron microscopy (SEM) coupled with energy-dispersive X-ray analysis, and Fourier-transform infrared spectroscopy (FTIR). The biosorbents were employed for the removal of methylene blue (MB) and crystal violet (CV) cationic dyes (as model organic pollutants) from aqueous solutions. The kinetic adsorption data mainly followed the pseudo-first-order model. The maximum adsorption capacities of the produced biosorbents ranged from 102 to 110 mg/g and were observed at 330 K. Equilibrium data for adsorption were fitted to Langmuir and Freundlich isotherm models. The calculated values of thermodynamic parameters suggested that the investigated adsorption processes were exergonic (ΔG < 0) and exothermic (ΔH < 0). In addition, a possible valorization of the cost-effective and eco-friendly spent biosorbents was tested by performing secondary adsorption of the anionic dyes.

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Section: Adsorption Isothermsmentioning

confidence: 99%

An Eco-Friendly Modification of a Walnut Shell Biosorbent for Increased Efficiency in Wastewater Treatment

Enache¹,

Samoilă²,

Cojocaru³

et al. 2023

Sustainability

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“…As a consequence, a larger amount of MB is adsorbed from the aqueous solution, but the amount of MB per unit mass of CMC-Mn-S2 adsorbent decreases. Because the MB dye is a well-known exogenous fluorophore [53], its homogeneous adsorption on the CMC-Mn-S2 surface was highlighted by using polarized light microscopy (Figure 8b). Moreover, the penetration into the depth of the beads of MB molecules was proved by the cross-section image (given in Figure S4, Supplementary Materials) due to the porous network created by the SDS surfactant's introduction during the gelation process.…”

Section: Kinetics and Isothermsmentioning

confidence: 99%

Magnetic Ionotropic Hydrogels Based on Carboxymethyl Cellulose for Aqueous Pollution Mitigation

Enache¹,

Grecu²,

Samoilă³

et al. 2023

Gels

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In this work, stabilized ionotropic hydrogels were designed using sodium carboxymethyl cellulose (CMC) and assessed as inexpensive sorbents for hazardous chemicals (e.g., Methylene Blue, MB) from contaminated wastewaters. In order to increase the adsorption capacity of the hydrogelated matrix and facilitate its magnetic separation from aqueous solutions, sodium dodecyl sulfate (SDS) and manganese ferrite (MnFe2O4) were introduced into the polymer framework. The morphological, structural, elemental, and magnetic properties of the adsorbents (in the form of beads) were assessed using scanning electron microscopy (SEM), energy-dispersive X-ray analysis, Fourier-transform infrared spectroscopy (FTIR), and a vibrating-sample magnetometer (VSM). The magnetic beads with the best adsorption performance were subjected to kinetic and isotherm studies. The PFO model best describes the adsorption kinetics. A homogeneous monolayer adsorption system was predicted by the Langmuir isotherm model, registering a maximum adsorption capacity of 234 mg/g at 300 K. The calculated thermodynamic parameter values indicated that the investigated adsorption processes were both spontaneous (ΔG < 0) and exothermic (ΔH < 0). The used sorbent can be recovered after immersion in acetone (93% desorption efficiency) and re-used for MB adsorption. In addition, the molecular docking simulations disclosed aspects of the mechanism of intermolecular interaction between CMC and MB by detailing the contributions of the van der Waals (physical) and Coulomb (electrostatic) forces.

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“…39−41 The two energy-release processes are fluorescence emission and the photothermal effect. 42 While fluorescence emission is one of the most fundamental bases for photosensitizers in applications such as tumor imaging and angiography, 42,43 the conversion from light to heat provides a bridge for the binding of photosensitizers to liposomes, thereby forming photoresponsive liposomes with multiple functions. Near-infrared (NIR) photoresponsive organic photosensitizers are the most widely used among many organic photosensitizers.…”

Section: Photoresponsive Liposomes Based On Organic Photosensitizersmentioning

confidence: 99%

“…Then, most of the energy is released through radiation and nonradiation transitions, and the molecules decay to the ground state. − The two energy-release processes are fluorescence emission and the photothermal effect . While fluorescence emission is one of the most fundamental bases for photosensitizers in applications such as tumor imaging and angiography, , the conversion from light to heat provides a bridge for the binding of photosensitizers to liposomes, thereby forming photoresponsive liposomes with multiple functions.…”

Section: Photoresponsive Liposomes Based On Organic Photosensitizersmentioning

confidence: 99%

Recent Development of Photoresponsive Liposomes Based on Organic Photosensitizers, Au Nanoparticles, and Azobenzene Derivatives for Nanomedicine

Pan

et al. 2022

ACS Appl. Nano Mater.

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Photoresponsive liposomes are controlled-release liposomes modified by photoresponsive materials. They use light with high spatial and temporal precision as a means of regulation. After excitation by light at appropriate wavelengths, photoresponsive liposomes can influence the permeability of phospholipid bilayers through the photothermal or photoisomerization effect of photoresponsive materials. The photothermal effect causes the phospholipid layer to become dispersed by local heating, and the photoisomerization effect modulates the release behavior by establishing permeation channels on the phospholipid layer through changes in the dipole moments of azobenzene derivatives. Currently, photoresponsive liposomes are widely used as a cutting-edge strategy for nanomedicine. In this paper, three types of photoresponsive liposomes based on organic photosensitizers, Au nanoparticles, and azobenzene derivatives with abundant research reports were introduced based on the differences in the photoresponse principles and forms of different photoresponsive materials. This review aims to reflect the differences in the structures, properties, photoresponsive materials, and principles of action of different photoresponsive liposomes. Moreover, this work outlines the current status of their application in the field of nanomedicine.

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Fluorescence Polarization Imaging of Methylene Blue Facilitates Quantitative Detection of Thyroid Cancer in Single Cells

Cited by 8 publications

References 44 publications

An Eco-Friendly Modification of a Walnut Shell Biosorbent for Increased Efficiency in Wastewater Treatment

An Eco-Friendly Modification of a Walnut Shell Biosorbent for Increased Efficiency in Wastewater Treatment

Magnetic Ionotropic Hydrogels Based on Carboxymethyl Cellulose for Aqueous Pollution Mitigation

Recent Development of Photoresponsive Liposomes Based on Organic Photosensitizers, Au Nanoparticles, and Azobenzene Derivatives for Nanomedicine

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