2012
DOI: 10.1002/cphc.201100921
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Fluorescence Properties of Carba Nicotinamide Adenine Dinucleotide for Glucose Sensing

Abstract: Carba nicotinamide adenine dinucleotide (cNAD) may serve as a stable cofactor for the enzyme-based detection of glucose. Many characteristics of cNAD and its reduced form cNADH resemble those of NAD and NADH, respectively. The fluorescence lifetimes of cNADH are determined to be 0.32(2) ns and 0.66(3) ns compared to 0.28(2) ns and 0.60(3) ns for NADH, and the temperature dependence of these lifetimes hints towards identical processes for quenching. The maximum emission occurs at 464 nm for both cNADH and NADH … Show more

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Cited by 11 publications
(10 citation statements)
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“…We reasoned that introduction of a "turn-on" mechanism in these dyes would be possible by implementing the unique spectral properties of the NAD(P) + /NAD(P)H pair. 18 When NAD(P) + is reduced to NAD(P)H, the nicotinamide moiety is transformed to a dihydronicotinamide. As a result, the absorption band at 260 nm decreases and a new maximum, resulting from the dihydronicotinamide moiety, occurs at 360 nm.…”
Section: ■ Results and Discussionmentioning
confidence: 99%
“…We reasoned that introduction of a "turn-on" mechanism in these dyes would be possible by implementing the unique spectral properties of the NAD(P) + /NAD(P)H pair. 18 When NAD(P) + is reduced to NAD(P)H, the nicotinamide moiety is transformed to a dihydronicotinamide. As a result, the absorption band at 260 nm decreases and a new maximum, resulting from the dihydronicotinamide moiety, occurs at 360 nm.…”
Section: ■ Results and Discussionmentioning
confidence: 99%
“…The high wavenumber indicates that the cation-anion hydrogen bond is relatively weak. It can be related to the interaction strength of water and methanol in similar ionic liquids including BF 4 − anions 22 23 . However, the vibrational band is not symmetric and exhibits additional intensity at the low frequency site.…”
mentioning
confidence: 99%
“…Later reports showed an enzymatic reduction for the first time, using a glucose dehydrogenase plus an enzymatic synthesis route of carba‐NAD + patented by Roche Diagnostics [26] . carba‐NAD(H) turned out to be an advantageous alternative to NAD(P)/H for glucose dehydrogenase‐based glucose sensing and found its way towards an application [27] . Interestingly, recent reports claim that the enzyme stability of glucose dehydrogenases increases significantly when stored together with the stable carba analog [28] .…”
Section: Introductionmentioning
confidence: 99%