Fluorescence quenching/enhancement surface assays: Signal manipulation using silver-coated gold nanoparticles

Matveeva, Evgenia G.; Shtoyko, Tanya; Gryczyński, Ignacy; Akopova, Irina; Gryczyński, Zygmunt

doi:10.1016/j.cplett.2008.01.075

Cited by 32 publications

(22 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although it is sought to obtain localized excitation and increased quantum yield near metal NPs but in practical both enhancing and quenching of RE ions or fluorophores (as the case may be) takes place near the metal particles [42]. Literature reports that to obtain enhancement of fluorescence, the fluorophore molecule must be situated at least at a distance of 50 Å or more from the metal surface to evade quenching at shorter distances [43].…”

Section: Resultsmentioning

confidence: 99%

“…In fact, the interactions between RE ions (or fluorophore molecules) with metals depend on a several factors like metal type, fluorophore type, the sizes of plasmonic metal NPs, distance between fluorophore and metal particle surface, and molecule dipole orientation versus particle surface, etc. and is fairly complex [42,44]. For example, nano-sized metallic particles have larger field effects than continuous metallic surfaces [43].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Surface Plasmon Resonance and Enhanced Fluorescence Application of Single-step Synthesized Elliptical Nano Gold-embedded Antimony Glass Dichroic Nanocomposites

2010

View full text Add to dashboard Cite

A novel series of elliptical gold (Au 0 ) nanoparticles (18-40 nm) embedded antimony glass (K 2 O-B 2 O 3 -Sb 2 O 3 ) dichroic nanocomposites have been synthesized by a single-step melt-quench in-situ thermochemical reduction technique. X-ray and selected area electron diffractions manifest growth of Au 0 nanoparticles along the (111) and (200) crystallographic planes. The transmission electron microscopic image reveals elliptical Au 0 nanoparticles having an aspect ratio varying in the range 1.2-2.1. The dichroic behavior of the nanocomposites arises due to elliptical shape of the Au 0 nanoparticles. These nanocomposites show strong surface plasmon resonance (SPR) band of Au nanoparticles in the range 610-681 nm and it exhibit red-shifts with increasing Au concentration. They, when co-doped with Sm 2 O 3 and excited at 949 nm, exhibit about sevenfold enhancement of the upconverted red emission transition of 4 G 5/2 → 6 H 9/2 at 636 nm due to local electric field enhancement effect of Au 0 nanoparticles induced by its SPR. These nanocomposites are the promising materials for laser, display, and various nanophotonic applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Surface Plasmon Resonance and Enhanced Fluorescence Application of Single-step Synthesized Elliptical Nano Gold-embedded Antimony Glass Dichroic Nanocomposites

2010

View full text Add to dashboard Cite

show abstract

“…Although localized excitation and increased quantum yield near metal NPs are desired but practically both enhancing and quenching of RE ions or fl uorophores (as the case may be) takes place near the metal particles [36,37]. It has been well documented in literature that to achieve enhancement of fluorescence, the fluorophore molecule must be located at a distance of 50 Å or more from the metal surface to avoid quenching at a distance shorter than this [35].…”

Section: Resultsmentioning

confidence: 99%

“…[37 40]. For example, small sized metallic particles have larger fi eld effects than continuous metallic surfaces [37]. However, it is very diffi cult to precisely predict the effect of each such parameter on the fluorescence properties of Sm 3+ ions with KBS glasses doped with Au Ag alloy nanoclusters.…”

Section: Resultsmentioning

confidence: 99%

Core-shell Au-Ag nanoparticles in dielectric nanocomposites with plasmon-enhanced fluorescence: A new paradigm in antimony glasses

Som

Karmakar

2009

Nano Res.

View full text Add to dashboard Cite

The nano era demands the synthesis of new nanostructured materials, if possible by simplified techniques, with remarkable properties and versatile applications. Here, we demonstrate a new single-step reproducible melt-quench methodology to fabricate core-shell bimetallic (Au 0 Ag 0 ) nanoparticles (28 89 nm) embedded glasses (dielectrics) by the use of a new reducing glass matrix, K 2 O B 2 O 3 Sb 2 O 3 (KBS) without applying any external reducing agent or multiple processing steps. The surface plasmon resonance (SPR) band of these nanocomposites embedded in KBS glass is tunable in the range 554-681 nm. More remarkably, taking advantage of the selective reduction capability of Sb 2 O 3 , this single-step methodology is used to fabricate inter-metallic: rare-earth ions co-embedded (Au Ag:Sm 3+ ) dielectric (glass)-based-dnanocomposites and study the effect of enhanced local fi eld on the red upconversion fl uorescence of Sm 3+ ions at 636 nm. The enhancement is found to be about 2 folds. This single-step in-situ selective reduction approach can be used to fabricate a variety of hybrid-nanocomposite devices for laser based applications (see supplementary information).

show abstract

“…However, there is a general concern on the fluorescence technique: a protein can contain multiple tryptophan and tyrosine residues. Depending on their actual distance to the gold metal core, the fluorescence of these amino acid residues can be quenched or enhanced [23,24]. This problem could cause uncertainty in the quantitative analysis.…”

Section: Introductionmentioning

confidence: 99%

Dynamic light scattering for gold nanorod size characterization and study of nanorod–protein interactions

2012

View full text Add to dashboard Cite

In recent years, there has been considerable interest and research activity in using gold nanoparticle materials for biomedical applications including biomolecular detection, bioimaging, drug delivery, and photothermal therapy. In order to apply gold nanoparticles in the real biological world, we need to have a better understanding of the potential interactions between gold nanoparticle materials and biomolecules in vivo and in vitro. Here, we report the use of dynamic light scattering (DLS) for gold nanorods characterization and nanorod-protein interaction study. In the size distribution diagram, gold nanorods with certain aspect ratios exhibit two size distribution peaks, one with an average hydrodynamic diameter at 5-7 nm, and one at 70-80 nm. The small size peak is attributed to the rotational diffusion of the nanorods instead of an actual dimension of the nanorods. When proteins are adsorbed to the gold nanorods, the average particle size of the nanorods increases and the rotational diffusion-related size distribution peak also changes dramatically. We examined the interaction between four different proteins, bovine serum albumin, human serum albumin, immunoglobulin G, and immunoglobulin A (IgA) with four gold nanorods that have the same diameter but different aspect ratios. From this study, we found that protein adsorption to gold nanorods is strongly dependent on the aspect ratio of the nanorods, and varies significantly from protein to protein. The two serum albumin proteins caused nanorod aggregation upon interaction with the nanorods, while the two immunoglobulin proteins formed a stable protein corona on the nanorod surface without causing significant nanorod aggregation. This study demonstrates that DLS is a valuable tool for nanorod characterization. It reveals information complementary to molecular spectroscopic techniques on gold nanorod-protein interactions.

show abstract

Fluorescence quenching/enhancement surface assays: Signal manipulation using silver-coated gold nanoparticles

Cited by 32 publications

References 36 publications

Surface Plasmon Resonance and Enhanced Fluorescence Application of Single-step Synthesized Elliptical Nano Gold-embedded Antimony Glass Dichroic Nanocomposites

Surface Plasmon Resonance and Enhanced Fluorescence Application of Single-step Synthesized Elliptical Nano Gold-embedded Antimony Glass Dichroic Nanocomposites

Core-shell Au-Ag nanoparticles in dielectric nanocomposites with plasmon-enhanced fluorescence: A new paradigm in antimony glasses

Dynamic light scattering for gold nanorod size characterization and study of nanorod–protein interactions

Contact Info

Product

Resources

About