Fluorescence quenching of 7-amino-4-methylcoumarin by different TEMPO derivatives

Żamojć, Krzysztof; Wiczk, Wiesław; Zaborowski, Bartłomiej; Jacewicz, Dagmara; Chmurzyński, Lech

doi:10.1016/j.saa.2014.10.102

Cited by 26 publications

(14 citation statements)

References 35 publications

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“…In addition, degradation or metabolization of compounds during their incubation with the E. coli detection culture could affect their fluorescent properties. To assess such potential effects (which clearly must be distinguished and accounted for) we investigated the interference caused by the model fluorescent compound 7-amino-4-methylcoumarin (7-AMC) and fluorescence-quenching compound rifampicin (Richter et al, 2015; Żamojć et al, 2015). As anticipated, these compounds respectively increased and decreased fluorescence recorded in the assay ( Figure 5 ).…”

Section: Resultsmentioning

confidence: 99%

High Throughput Screening Method for Identifying Potential Agonists and Antagonists of Arabidopsis thaliana Cytokinin Receptor CRE1/AHK4

Klimeš¹,

Turek²,

Mazura³

et al. 2017

Front. Plant Sci.

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The CRE1/AHK4 cytokinin receptor is an important component of plants’ hormone signaling systems, and compounds that can alter its activity have potential utility for studying the receptor’s functions and/or developing new plant growth regulators. A high throughput method was developed for screening compounds with agonist or antagonist properties toward the CRE1/AHK4 cytokinin receptor in a single experiment using the Nanodrop II liquid handling system and 384-well plates. Potential ligands are screened directly, using a reporter system in which receptor signaling activity triggers expression of β-galactosidase in Escherichia coli. This enzyme generates a fluorescent product from a non-fluorescent substrate, allowing the agonistic/antagonistic behavior of tested compounds to be assayed in relation to that of an internal standard (here the natural ligand, trans-zeatin). The method includes a robust control procedure to determine false positive or false negative effects of the tested compounds arising from their fluorescent or fluorescent-quenching properties. The presented method enables robust, automated screening of large libraries of compounds for ability to activate or inhibit the Arabidopsis thaliana cytokinin receptor CRE1/AHK4.

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Section: Resultsmentioning

confidence: 99%

High Throughput Screening Method for Identifying Potential Agonists and Antagonists of Arabidopsis thaliana Cytokinin Receptor CRE1/AHK4

Klimeš¹,

Turek²,

Mazura³

et al. 2017

Front. Plant Sci.

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“…Photoluminescence emission (PL) spectrum was obtained on a fluorescence spectrophotometer (Hitachi, F4600) at the scope of 400–600 nm with a photomultiplier tube handled at 800 volt. A 150 watt xenon lamp was served as the pumping source, at an excitation wavelength of 340 nm (Zamojć et al, 2015 ). The emission and excitation slits size were 5 nm and scan speed was setting at 240 nm per min.…”

Section: Methodsmentioning

confidence: 99%

Fabrication of an AMC/MMT Fluorescence Composite for its Detection of Cr(VI) in Water

Wei

Mei

et al. 2018

Front. Chem.

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Hexavalent chromium species, Cr(VI), which can activate teratogenic processes, disturb DNA synthesis and induce mutagenic changes resulting in malignant tumors. The detection and quantification of Cr(VI) is very necessary. One of the rapid and simple methods for contaminant analysis is fluorescence detection using organic dye molecules. Its application is limited owing to concentration quenching due to aggregation of fluorescent molecules. In this study, we successfully intercalated 7-amino-4-methylcoumarin (AMC) into the interlayer space of montmorillonite (MMT), significantly inhibited fluorescence quenching. Due to enhanced fluorescence property, the composite was fabricated into a film with chitosan to detect Cr(VI) in water. Cr(VI) can be detected in aqueous solution by instruments excellent, ranging from 0.005 to 100 mM with a detection limit of 5 μM.

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“…We wanted to show (in comparison to our earlier investigations) that the affinity of the peptides to metal ions depends not only on the primary structure (amino acid composition) but also -to a large extent -on the shape of the adopted peptide conformation. We report herein the type of interactions between the peptide fragment of the Aβ polypeptide, Aβ (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16) : Ac-Arg-His-AspSer-Gly-Tyr-Glu-Val-His-His-Gln-Lys-NH 2 hereafter referred to as HZ1, and Cu 2+ ions.…”

Section: Introductionmentioning

confidence: 99%

“…The studies on the fluorescence quenching performed in our group with the use of steady state and transient methods provided a large quantity of information, mainly about fluorescent dyes [12][13][14][15]. Additionally, such a technique has been widely used for acquiring qualitative and quantitative information on peptides containing fluorescent amino acids (tyrosine, phenylalanine and tryptophan) as quenched by paramagnetic metal ions (Mn 2+ , Co 2+ , Cu 2+ ) [11,[16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Probing the binding of Cu 2+ ions to a fragment of the Aβ (1–42) polypeptide using fluorescence spectroscopy, isothermal titration calorimetry and molecular dynamics simulations

Makowska

Żamojć

Wyrzykowski

et al. 2016

Biophysical Chemistry

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Fluorescence quenching of 7-amino-4-methylcoumarin by different TEMPO derivatives

Cited by 26 publications

References 35 publications

High Throughput Screening Method for Identifying Potential Agonists and Antagonists of Arabidopsis thaliana Cytokinin Receptor CRE1/AHK4

High Throughput Screening Method for Identifying Potential Agonists and Antagonists of Arabidopsis thaliana Cytokinin Receptor CRE1/AHK4

Fabrication of an AMC/MMT Fluorescence Composite for its Detection of Cr(VI) in Water

Probing the binding of Cu 2+ ions to a fragment of the Aβ (1–42) polypeptide using fluorescence spectroscopy, isothermal titration calorimetry and molecular dynamics simulations

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