Fluorescence quenching of biologically active carboxamide by aniline and carbon tetrachloride in different solvents using Stern–Volmer plots

Patil, N. R.; Melavanki, Raveendra; Kapatkar, S. B.; Chandrashekhar, K.; Patil, H D; Umapathy, Siva

doi:10.1016/j.saa.2011.05.104

Cited by 21 publications

(6 citation statements)

References 15 publications

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“…(4), (5) and (6) the values of V and r are determined by least square fit method in all the systems and the values of V and r are given in Table 1. Similar results were also obtained by others [5][6][7][8].…”

Section: Resultssupporting

confidence: 91%

“…3. Similar experimental results were also observed by others [5][6][7][8]. Thus, positive deviation from linearity suggests that quenching mechanism is not purely collisional and this may be attributed either to the ground state complex formation or sphere of action static quenching models [1][2].…”

Section: Resultssupporting

confidence: 83%

“…From Table 1, we see that the values of kinetic distance (r) are larger than the encounter distance (R) indicating that the sphere of action model holds good in our case also. Further it may also be noted that a positive deviation in the S-V plot is expected when both static and dynamic quenching occurs simultaneously [5][6][7][8]. In order to find out whether the reactions are diffusion limited, we invoke the finite sink approximation model.…”

Section: Resultsmentioning

confidence: 99%

“…Fluorescence quenching of organic molecules in solution by various quenchers like carbon tetrachloride, bromobenzene, aniline, halide ions, metal ions etc. has been studied by several investigators [1][2][3][4][5][6][7][8][9][10]. The study has been mainly to understand the nature of bimolecular reactions taking place both under steady state and transient conditions.…”

Section: Introductionmentioning

confidence: 99%

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Solvent Effect on Fluorescence Quenching of 7, 8 Benzo‑4‑azido Methyl Coumarin by Aniline

et al. 2013

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Fluorescence quenching of biologically active studies of 7, 8 benzo-4-azidomethyl coumarin (7BAMC) by aniline in four different organic solvents namely benzene, dioxane, tetrahydrofuran and acetonitrile has been carried out at room temperature with a view to understand the quenching mechanisms. The Stern–Volmer (S-V) plot has been found to be non-linear with a positive deviation for all the solvents studied. In order to interpret these results we have invoked the ground state complex formation and sphere of action static quenching models. Using these models various quenching rate parameters have been determined. The magnitudes of these parameters suggest that sphere of action static quenching model agrees well with the experimental results. Hence the positive deviation is attributed to the static and dynamic quenching. Further, with the use of Finite Sink approximation model, it was possible to check these bimolecular reactions as diffusion-limited and to estimate independently distance parameter R’ and mutual diffusion coefficient D. Finally an effort has been made to correlate the values of R’ and D with the values of the encounter distance R and the mutual coefficient D determined using the Edward’s empirical relation and Stokes – Einstein relation.

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Solvent Effect on Fluorescence Quenching of 7, 8 Benzo‑4‑azido Methyl Coumarin by Aniline

et al. 2013

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“…The first process will be reflected in a reduced lifetime of the fluorophore, and the second process by a reduced brightness. A plot of F 0 /F vs quencher concentration (called the “Stern-Volmer” plot ( Patil et al, 2011 ), where F 0 is the steady-state fluorescence at zero concentration of the quencher, and F is the fluorescence at a given quencher concentration) can quantify the quenching process. A separate measurement of the lifetime can reveal how much of the quenching is due to fast collisional processes, and how much is due to ground state complex formation.…”

Section: Methodsmentioning

confidence: 99%

Single Molecule Measurements of the Accessibility of Molecular Surfaces

Dey

Vishvakarma

Das

et al. 2021

Front. Mol. Biosci.

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An important measure of the conformation of protein molecules is the degree of surface exposure of its specific segments. However, this is hard to measure at the level of individual molecules. Here, we combine single molecule photobleaching (smPB, which resolves individual photobleaching steps of single molecules) and fluorescence quenching techniques to measure the accessibility of individual fluorescently labeled protein molecules to quencher molecules in solution. A quencher can reduce the time a fluorophore spends in the excited state, increasing its photostability under continuous irradiation. Consequently, the photo-bleaching step length would increase, providing a measure for the accessibility of the fluorophore to the solvent. We demonstrate the method by measuring the bleaching step-length increase in a lipid, and also in a lipid-anchored peptide (both labelled with rhodamine-B and attached to supported lipid bilayers). The fluorophores in both molecules are expected to be solvent-exposed. They show a near two-fold increase in the step length upon incubation with 5 mM tryptophan (a quencher of rhodamine-B), validating our approach. A population distribution plot of step lengths before and after addition of tryptophan show that the increase is not always homogenous. Indeed there are different species present with differential levels of exposure. We then apply this technique to determine the solvent exposure of membrane-attached N-terminus labelled amylin (h-IAPP, an amyloid associated with Type II diabetes) whose interaction with lipid bilayers is poorly understood. hIAPP shows a much smaller increase of the step length, signifying a lower level of solvent exposure of its N-terminus. Analysis of results from individual molecules and step length distribution reveal that there are at least two different conformers of amylin in the lipid bilayer. Our results show that our method (“Q-SLIP”, Quenching-induced Step Length increase in Photobleaching) provides a simple route to probe the conformational states of membrane proteins at a single molecule level.

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Effect of Heavy Metals on Dynamic and Static Quenching of the Fluorescence of the Host-Guest Inclusion Complex Methyl-β-Cyclodextrin by 2,9-Dimethyl-4,7-Diphenyl-1,10-Phenanthroline in Aqueous Media

2021

J Appl Spectrosc

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Fluorescence quenching of biologically active carboxamide by aniline and carbon tetrachloride in different solvents using Stern–Volmer plots

Cited by 21 publications

References 15 publications

Solvent Effect on Fluorescence Quenching of 7, 8 Benzo‑4‑azido Methyl Coumarin by Aniline

Solvent Effect on Fluorescence Quenching of 7, 8 Benzo‑4‑azido Methyl Coumarin by Aniline

Single Molecule Measurements of the Accessibility of Molecular Surfaces

Effect of Heavy Metals on Dynamic and Static Quenching of the Fluorescence of the Host-Guest Inclusion Complex Methyl-β-Cyclodextrin by 2,9-Dimethyl-4,7-Diphenyl-1,10-Phenanthroline in Aqueous Media

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