Fluorescence quenching of fluorescein by Merocyanine 540 in liposomes

Toprak, Mahmut; Aydın, Burcu Meryem; Arık, Mustafa; Onganer, Yavuz

doi:10.1016/j.jlumin.2011.05.049

Cited by 26 publications

(17 citation statements)

References 25 publications

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“…The Fluorescence quantum yields of donor molecules were calculated through the Parker-Rees equation: where D is the integrated area under the corrected fluorescence spectrum, n is the refractive index of the solution, and OD is the optical density at the excitation wavelength ( ex = 350 nm). The subscripts s and r refer to the sample and reference solutions, respectively [21]. Quinine sulfate in 0.5 M H 2 SO 4 solution was used as the reference.…”

Section: Apparatusmentioning

confidence: 99%

A novel system for Fe3+ ion detection based on fluorescence resonance energy transfer

Bozkurt

Arık

Onganer

2015

Sensors and Actuators B: Chemical

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Section: Apparatusmentioning

confidence: 99%

A novel system for Fe3+ ion detection based on fluorescence resonance energy transfer

Bozkurt

Arık

Onganer

2015

Sensors and Actuators B: Chemical

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“…Indeed, their membranes offer opportunities for an efficient functionalization and for this reason they find various biological applications, including drug delivery, labeling, and bioimaging . In spite of extensive studies on SUVs, only a few works have been focused on their capability to nanostructure organic dyes . Moreover, there is a strong interest toward new vesicular formulations, made by nonlipid components, able to overcome the intrinsic instability of liposomes that hinders their biomedical applications …”

mentioning

confidence: 99%

Nanostructuring Lipophilic Dyes in Water Using Stable Vesicles, Quatsomes, as Scaffolds and Their Use as Probes for Bioimaging

Ardizzone

Illa-Tuset

Faraudo

et al. 2018

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A new kind of fluorescent organic nanoparticles (FONs) is obtained using quatsomes (QSs), a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines (1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (DiI), 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indodicarbocyanine perchlorate (DiD), and 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indotricarbocyanine iodide (DiR)) in aqueous media. The obtained FONs, prepared by a CO -based technology, show excellent colloidal- and photostability, outperforming other nanoformulations of the dyes, and improve the optical properties of the fluorophores in water. Molecular dynamics simulations provide an atomistic picture of the disposition of the dyes within the membrane. The potential of QSs for biological imaging is demonstrated by performing superresolution microscopy of the DiI-loaded vesicles in vitro and in cells. Therefore, fluorescent QSs constitute an appealing nanomaterial for bioimaging applications.

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“…Consequently, the thermodynamic parameters were evaluated in order to understand better the acting forces between ligand and β-LG [6]. The changes of enthalpy (ΔH 0 ) and entropy (ΔS 0 ) are the main arguments confirming the interaction mode of the ligand with protein [44]. The acting forces between proteins and ligands were classified on the basis of the magnitude and the sign of thermodynamic parameters into four groups of noncovalent interactions: (a) ΔH 0 >0 and ΔS 0 >0, hydrophobic interactions; (b) ΔH 0 <0 and ΔS 0 <0, van der [49].…”

Section: Analysis Of Binding and Of Its Thermodynamicsmentioning

confidence: 99%

“…Since over the temperature range studied, the enthalpy and entropy are constant or do not change significantly, it is possible to use the van't Hoff equation (Eq. (5)) in order to evaluate the thermodynamic parameters [44]:…”

Section: Analysis Of Binding and Of Its Thermodynamicsmentioning

confidence: 99%

Probing of the Interaction Between β-Lactoglobulin and the Anticancer Drug Oxaliplatin

Ghalandari¹,

Divsalar²,

Eslami-Moghadam³

et al. 2014

Appl Biochem Biotechnol

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The potential carrier role of β-lactoglobulin (β-LG) and its interactions with oxaliplatin were studied using various spectroscopic techniques (fluorescence, UV-visible, and circular dichroism (CD)) in an aqueous medium at two temperatures of 25 and 37 °C in combination with a molecular docking study. Fluorescence measurements have shown that the observed quenching is a combination of static and dynamic quenching with a predominant contribution of static mode. The presence of a single binding site located in the internal cavity of the β-barrel of β-LG was confirmed by molecular docking calculations. Thermodynamic data as well as molecular docking indicated that the hydrophobic interactions dominate in the binding site. Results of fluorescence resonance energy transfer (FRET) measurements in combination with docking results imply that resonance energy transfer occurs between β-LG and its ligand oxaliplatin. Additionally, CD results revealed that oxaliplatin binding has no influence on the β-LG structure. The molecular docking results indicate that docking may be an appropriate method for the prediction and confirmation of experimental results. Complementary molecular docking results may be useful for the determination of the binding mechanism of proteins such as β-LG in pharmaceutical and biophysical studies providing new insight in the novel pharmacology and new solutions in the formulation of advanced oral drug delivery systems.

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Fluorescence quenching of fluorescein by Merocyanine 540 in liposomes

Cited by 26 publications

References 25 publications

A novel system for Fe3+ ion detection based on fluorescence resonance energy transfer

A novel system for Fe3+ ion detection based on fluorescence resonance energy transfer

Nanostructuring Lipophilic Dyes in Water Using Stable Vesicles, Quatsomes, as Scaffolds and Their Use as Probes for Bioimaging

Probing of the Interaction Between β-Lactoglobulin and the Anticancer Drug Oxaliplatin

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