Fluorescence resonance energy transfer usage to track the sequence promoter changes in CGB5 gene in ovarian cancer patients

Andrusiewicz, Mirosław; Skibinska, Izabela; Gąsiorowska, Emilia; Białas, Piotr; Kotwicka, Małgorzata

doi:10.1016/j.biopha.2017.02.113

Cited by 3 publications

(3 citation statements)

References 37 publications

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“…CGB5 is highly expressed in ovarian cancer and lowly expressed in adjacent tissues, and can further facilitate the invasion of ovarian cancer cells by promoting angiogenesis (19). Its cancer-promoting effect is consistent with the trend of our analysis.…”

Section: Discussionsupporting

confidence: 90%

Screening of prognostic immune genes and establishment of prognostic model of cervical adenocarcinoma based on bioinformatics analysis

Sheng

Nie

2022

Ann Transl Med

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Background: There is currently a lack of clinical models to accurately predict the prognosis of cervical adenocarcinoma. This study aimed to explore the correlation between immune genes and the prognosis of cervical adenocarcinoma patients, and establish a prognostic model. Methods:Transcriptome sequencing data sets and clinical data sets of cervical adenocarcinoma samples were downloaded from the Gene Expression Omnibus (GEO) database. Information about the immune gene was obtained from the ImmPort database. Differentially expressed genes and differentially expressed immune genes were screened in cervical adenocarcinoma tissue and normal cervical group by edgeR package.Differentially expressed immune genes were screened for prognosis-related immune genes by Cox analysis. Taking the immune genes related to prognosis as variables, a prognosis prediction model was established by multivariate Cox regression analysis. Kaplan-Meier analysis and a receiver operating characteristic (ROC) curve were used to test the effectiveness of the model. According to the clinical information and risk score, univariate multivariate Cox analyses were used to screen the independent prognostic risk factors of cervical adenocarcinoma. Results: CXCL9 was an independent prognostic factor of cervical adenocarcinoma [hazard ratio (HR) =0.63; P=0.025]. CGB5 (HR =1.22; P=0.034), CXCL12 (HR =1.33; P=0.023), PTX3 (HR =1.53; P=0.024), and CXCL10 (HR =2.31; P=0.031) were prognostic risk factors for cervical adenocarcinoma. The risk score was calculated as follows: risk score = (0.005 × CXCL10) + (0.076 × CGB5) + (0.061 × CXCL12) + (0.034 × PTX3)+ (−0.004 × CXCL9). The prognosis of the low-risk score group was better than that of the high-risk score group (P=0.035). The area under the ROC curve (AUC) of the risk score was 0.713, and the predictive power was good. Multivariate Cox analysis showed that N stage (HR =1.34; P=0.035) and risk score (HR =1.37; P<0.001) were independent risk factors for the prognosis of cervical adenocarcinoma (HR >1; P<0.001).Conclusions: In this study, an immune gene prognosis prediction model for cervical adenocarcinoma was established based on the GEO and ImmPort databases. The prediction performance of the model is good, and the prognosis of patients can be evaluated according to the gene expression, which has clinical practicability.

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Section: Discussionsupporting

confidence: 90%

Screening of prognostic immune genes and establishment of prognostic model of cervical adenocarcinoma based on bioinformatics analysis

Sheng

Nie

2022

Ann Transl Med

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“…The role of CGB5 in tumors is of great interest. Previous studies have provided evidence to support the notion that CGB5 plays a critical factor in gastric cancer [Qin et al 2021;Ji et al 2023], ovarian cancer [Andrusiewicz et al 2017], and squamous lung cancer [Liu et al 2022] and is closely associated with patient prognosis. However, it is regrettable that there is currently no reported role of CGB5 across pan-cancer.…”

Section: Introductionmentioning

confidence: 94%

CGB5 Proves to be a Promising Predictive and Immunotherapeutic Indicator across Pan-Cancer

Chen,

Yu,

et al. 2023

Preprint

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CGB5 plays an important factor in numerous different cancers and is strongly associated with patient prognosis. Unfortunately, there is currently no data, however, on whether CGB5 plays a role in pan-cancer diseases. Research on CGB5 in pan-cancer has been conducted through multiple websites and public databases, including TCGA, HPA, UALCAN, cbiopportal Platform, UALCAN, GSCA, Kaplan-Meier Plotter, TIMER, TISIDB, SangerBox Website, and metscape. The genomic, transcriptomic, epigenetic, immune microenvironmental, and clinical prognostic significance of CGB5 across pan-cancer is investigated with the resulting outcome. CGB5 expression in gastric cancer was further detected, and the potential mechanism of its influence on prognosis was elucidated. This study found that abnormal CGB5 expression in pan carcinoma is correlated with a poorer prognosis. Aberrant CGB5 expression is potentially linked to gene mutations, copy number variation (CNV), and DNA methylation. In addition, this study revealed a robust association between the expression of CGB5 and immune cell infiltration across various types of cancer, with differences in cell type and level among distinct tumor types. In addition, the present investigation has shown that A strong association has been detected between the upregulation of CGB5 expression and immune cell infiltration in various forms of cancer, with differences in the different tumor types in the type and level of immune cell infiltration. Our further cell experiments also confirmed the upregulation of CGB5 within the context of gastric cancer. In conclusion, it has been noted that CGB5 expression exhibited a substantial rise across wide types of malignancies, and this upregulation was shown to exhibit a significant association with the prognosis of individuals who have cancer. Further studies showed that CGB5 expression in tumors affects the tumor immune microenvironment and promotes tumorigenesis. We also explored CGB5 expression in gastric cancer and clarified its potential prognostic mechanism. It reveals CGB5's significance in cancer diagnosis and treatment.

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“…As described previously, in short, during the melting phase, and FRET occurs if both fluorescent dyes are in approximate distance. 25 This absorbed excitation energy is transferred from the anchor to the sensor probe and the emitted by LC Red-640 fluorescence energy is detected. Gradual temperature-dependent fluorescence intensity decrease as the probes melt.…”

Section: Genetic Analysesmentioning

confidence: 99%

TIMP2 Polymorphisms Association With Curve Initiation and Progression of Thoracic Idiopathic Scoliosis in the Caucasian Females

Andrusiewicz

Harasymczuk

Janusz

et al. 2019

Journal Orthopaedic Research

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Idiopathic scoliosis (IS) etiology remains unclear, but strong genetic background is suggested. Previously reported TIMP2 study indicates an association of genic rs8179090 with IS progression in a Han Chinese population. However, there has been a lack of investigation into intragenic TIMP2 polymorphisms in IS patients. We recruited 100 Caucasian females with IS and 100 controls. Patients were subdivided accordingly to: progression rate, curve severity, joint mobility, and curve pattern. Allele‐specific‐polymerase chain reaction based on fluorescence resonance energy transfer was applied to evaluate nine TIMP2 polymorphisms. Distribution of genotype and allele frequency in only one polymorphism (rs11658743) differed in case–control study. Four of the polymorphisms (rs2277700, rs11077401, rs2376999, and rs4789934) showed non‐equal distributions either in genotype or/and allele distributions in the patients of different progression rates. The rs11077401 was related to curve severity patients distinction and the rs8179090 distinguished patients with different joint mobility level. Two polymorphisms either differed statistically in case of curve patterns subgrouping (rs8068674 and rs8179090) or showed a slight tendency toward significance in the recessive model of allele distributions (rs9916809 and rs8179090). The remaining two polymorphisms (rs2377005, rs11658743) showed no association with either clinical or radiographic IS characteristics. The influence of the G allele of the rs8179090 on the clinical course of IS has not yet been confirmed. We identified four TIMP2 polymorphisms (rs11077401, rs2376999, rs2277700, and rs4789934) that were associated with a higher risk of the progressive IS form. Further genetic association studies based on suggested clinical criteria would be necessary to validate TIMP2 polymorphisms associated with the curve progression. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2217–2225, 2019

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Fluorescence resonance energy transfer usage to track the sequence promoter changes in CGB5 gene in ovarian cancer patients

Cited by 3 publications

References 37 publications

Screening of prognostic immune genes and establishment of prognostic model of cervical adenocarcinoma based on bioinformatics analysis

Screening of prognostic immune genes and establishment of prognostic model of cervical adenocarcinoma based on bioinformatics analysis

CGB5 Proves to be a Promising Predictive and Immunotherapeutic Indicator across Pan-Cancer

TIMP2 Polymorphisms Association With Curve Initiation and Progression of Thoracic Idiopathic Scoliosis in the Caucasian Females

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