Fluorescence saturation spectroscopy in probing electronically excited states of silver nanoclusters

Volkov, Ivan L.; Sych, Tomash S.; Serdobintsev, P. Yu.; Reveguk, Zakhar V.; Kononov, Alexei I.

doi:10.1016/j.jlumin.2015.12.019

Cited by 14 publications

(18 citation statements)

References 28 publications

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“…The cluster on the CT DNA exhibited the same absorption cross-section and similar spectral properties as the reported earlier Ag 4 2+ cluster on the 12-mer DNA ( 74 , 76 ). As the spectral parameters of Ag clusters are extremely sensitive to size, charge, and shape ( 83–85 ), we believe that these Ag–DNA complexes have a similar metal acceptor core structures.…”

Section: Resultssupporting

confidence: 82%

“…The calculated excitation spectrum of the Ag 6 2+ cluster is shown in Supplementary Figure S10 . The predicted larger size of this cluster compared to the Ag 4 2+ cluster correlates well with the fact that it has two times higher experimental absorption cross-section (2.0 × 10 5 M −1 cm −1 ) than the Ag 4 2+ cluster (1.0 × 10 5 M −1 cm −1 ( 76 )).…”

Section: Resultssupporting

confidence: 72%

“…As seen in the figure, the UV band of the complex at 260 nm originates mainly from DNA absorption, while the band with a maximum at 485 nm is evidently from the lowest energy transition of the Ag cluster. The UV band observed in the excitation spectrum of the complex, as in other Ag–DNA complexes ( 62 , 74 , 76 ), is obviously due to energy transfer between DNA and the Ag cluster. Here, the excitation spectrum approaches its absorption spectrum, which indicates that the energy transfer efficiency is close to unity.…”

Section: Resultsmentioning

confidence: 84%

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DNA as UV light–harvesting antenna

Volkov

Reveguk

Serdobintsev

et al. 2017

Nucleic Acids Research

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The ordered structure of UV chromophores in DNA resembles photosynthetic light-harvesting complexes in which quantum coherence effects play a major role in highly efficient directional energy transfer. The possible role of coherent excitons in energy transport in DNA remains debated. Meanwhile, energy transport properties are greatly important for understanding the mechanisms of photochemical reactions in cellular DNA and for DNA-based artificial nanostructures. Here, we studied energy transfer in DNA complexes formed with silver nanoclusters and with intercalating dye (acridine orange). Steady-state fluorescence measurements with two DNA templates (15-mer DNA duplex and calf thymus DNA) showed that excitation energy can be transferred to the clusters from 21 and 28 nucleobases, respectively. This differed from the DNA–acridine orange complex for which energy transfer took place from four neighboring bases only. Fluorescence up-conversion measurements showed that the energy transfer took place within 100 fs. The efficient energy transport in the Ag–DNA complexes suggests an excitonic mechanism for the transfer, such that the excitation is delocalized over at least four and seven stacked bases, respectively, in one strand of the duplexes stabilizing the clusters. This result demonstrates that the exciton delocalization length in some DNA structures may not be limited to just two bases.

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Section: Resultssupporting

confidence: 82%

Section: Resultssupporting

confidence: 72%

Section: Resultsmentioning

confidence: 84%

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DNA as UV light–harvesting antenna

Volkov

Reveguk

Serdobintsev

et al. 2017

Nucleic Acids Research

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“…7 It has been previously demonstrated for a large number of DNA-AgNCs that dark state formation from the Frank-Condon state is a common process. [20][21][22][23][24][25][26][27] This dark state can be optically excited by a secondary NIR laser that pumps the dark state to the emissive state in a process termed optically activated delayed fluorescence (OADF). 20,23 We performed OADF measurements, since this allows to estimate the minimum value for the quantum yield of dark state formation (Q D1 ), as was previously demonstrated for a red-emitting DNA-AgNC.…”

mentioning

confidence: 99%

Unusually large fluorescence quantum yield for a near-infrared emitting DNA-stabilized silver nanocluster

et al. 2020

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“…21 It is known from literature that upon exciting DNA-AgNCs, the Franck-Condon state (FC) can evolve into a microsecond-lived dark state. 15,[22][23][24][25][26] Besides dark state formation, the FC state can either relax to the ground state (S 0 ) or evolve to the emissive state (S 1 ). 27 All these initial processes are ultrafast and occur on a sub-picosecond timescale.…”

mentioning

confidence: 99%

Anti-Stokes fluorescence microscopy using direct and indirect dark state formation

et al. 2018

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Measurements on biological samples are often hampered by auto-fluorescence from inherent compounds in tissue or cells, limiting the achievable contrast. Both the signal of interest and the auto-fluorescence are usually detected on the Stokes side of the excitation laser. In this communication, we present two new microscopy modalities, based on the emission of a red-emitting DNA-stabilized silver nanocluster (DNA-AgNC). Its bright fluorescence can be generated on the anti-Stokes side of the readout laser, allowing easy spectral separation of the signal of interest from the Stokes side auto-fluorescence.

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Fluorescence saturation spectroscopy in probing electronically excited states of silver nanoclusters

Cited by 14 publications

References 28 publications

DNA as UV light–harvesting antenna

DNA as UV light–harvesting antenna

Unusually large fluorescence quantum yield for a near-infrared emitting DNA-stabilized silver nanocluster

Anti-Stokes fluorescence microscopy using direct and indirect dark state formation

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