Fluorescence Sensing of Nucleic Acid by Curcumin Encapsulated Poly(Ethylene Oxide)-Block-Poly(Propylene Oxide)-Block-Poly(Ethylene Oxide) Based Nanocapsules

Bechnak, Linda; Kurdi, Riham El; Patra, Digambara

doi:10.1007/s10895-020-02528-9

Cited by 10 publications

(13 citation statements)

References 33 publications

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“…The knowledge of atomic structure needed to understand and describe the nature of the phenomenon was not acquired until the beginning of the 20th century. By providing detailed information, this technique has enormous advantages over classical microscopy techniques [ 67 ]. In fact, literature is plentiful of studies dealing with the design of new fluorescent probes such as (bio)sensors to detect (even with the naked eye) enzymes, metals, biomaterials and others.…”

Section: Fluorescent Probes In Ocular Applicationsmentioning

confidence: 99%

Fluorescent Nanosystems for Drug Tracking and Theranostics: Recent Applications in the Ocular Field

et al. 2022

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The greatest challenge associated with topical drug delivery for the treatment of diseases affecting the posterior segment of the eye is to overcome the poor bioavailability of the carried molecules. Nanomedicine offers the possibility to overcome obstacles related to physiological mechanisms and ocular barriers by exploiting different ocular routes. Functionalization of nanosystems by fluorescent probes could be a useful strategy to understand the pathway taken by nanocarriers into the ocular globe and to improve the desired targeting accuracy. The application of fluorescence to decorate nanocarrier surfaces or the encapsulation of fluorophore molecules makes the nanosystems a light probe useful in the landscape of diagnostics and theranostics. In this review, a state of the art on ocular routes of administration is reported, with a focus on pathways undertaken after topical application. Numerous studies are reported in the first section, confirming that the use of fluorescent within nanoparticles is already spread for tracking and biodistribution studies. The first section presents fluorescent molecules used for tracking nanosystems’ cellular internalization and permeation of ocular tissues; discussions on the classification of nanosystems according to their nature (lipid-based, polymer-based, metallic-based and protein-based) follows. The following sections are dedicated to diagnostic and theranostic uses, respectively, which represent an innovation in the ocular field obtained by combining dual goals in a single administration system. For its great potential, this application of fluorescent nanoparticles would experience a great development in the near future. Finally, a brief overview is dedicated to the use of fluorescent markers in clinical trials and the market in the ocular field.

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Section: Fluorescent Probes In Ocular Applicationsmentioning

confidence: 99%

Fluorescent Nanosystems for Drug Tracking and Theranostics: Recent Applications in the Ocular Field

et al. 2022

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“…[23] Moreover, a study established by Beshnak and colleagues proved the ability of curcumin polymeric nanocapsules to detect DNA and RNA simultaneously. [9] Therefore, Cur-DBPC-COL NCs were tested to detect DNA. Interestingly, the increase in the DNA concentration did not alter the emission intensity (see Figure 6a).…”

Section: Lod Concentration Range Referencesmentioning

confidence: 99%

“…[8] Consequently, curcumin nanocapsules were found to be a relative nanosensor to detect RNA. [9] Curcumin is a yellow pigment extracted from the rhizomes of Curcuma longa. This gold-coloured phytochemical has been widely used in culinary applications, food colouring and medicinal applications.…”

mentioning

confidence: 99%

“…[22] In addition, when curcumin was encapsulated into poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide), the detection of both RNA and DNA was achieved. [9] Being a fluorescence transducer, curcumin has been used in biomedical applications to detect RNA and DNA. [23] Physiochemical properties of curcumin can be extremely improved after being encapsulated in liposomes.…”

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confidence: 99%

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Curcumin‐embedded DBPC liposomes coated with chitosan layer as a fluorescence nanosensor for the selective detection of ribonucleic acid

2022

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One of the limitations of fluorescence probe molecules during biomedical estimation is their lack of ability to selectively determine the targeted species. To overcome this there have been various approaches that involve attaching a functional group or aptamers to the fluorescence probe. However, encapsulating probe molecules in a matrix using nanotechnology can be a viable and easier method. Curcumin (Cur) as a fluorescence marker cannot distinguish DNA and RNA. This research reports a novel selective approach involving the use of nanocapsules composed of liposomal curcumin coated with chitosan for the selective detection of RNA molecules using a fluorescence method. The increase in RNA concentration enhanced the electrostatic interaction between the negatively charge surface of RNA and the positively charged nanocapsule, which was further verified by zeta potential measurement. This method had a low limit of detection (36 ng/ml) and higher linear dynamic ranges compared with other studies found in the literature. Moreover, the method was not affected by DNA and was selective for the detection of RNA molecules for which the site of interaction was confined only to uracil. The selectivity for RNA molecules towards other analogues species was also examined and recovery range found was between 99 and 100.33%.

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“…Over the last decades, nanoparticles have gained great attention in medical sciences [ 1 , 2 , 3 ] especially in the fields of imaging [ 4 , 5 , 6 , 7 , 8 , 9 , 10 ], sensing [ 11 , 12 , 13 , 14 ], gene delivery systems [ 15 , 16 , 17 , 18 , 19 , 20 , 21 ] and drug delivery [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. Several studies have focused on the development of polymeric nanoparticles as drug delivery systems.…”

Section: Introductionmentioning

confidence: 99%

Lyophilization of Nanocapsules: Instability Sources, Formulation and Process Parameters

Degobert

Aydin

2021

Pharmaceutics

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Polymeric nanocapsules have gained more and more interest in the medical sciences. Their core-shell structure offers numerous advantages, especially regarding their use as drug delivery systems. This review begins by presenting the different intrinsic sources of the instability of nanocapsules. The physical and chemical potential instabilities of nanocapsules reduce their shelf-life and constitute a barrier to their clinical use and to their commercialization. To overcome these issues, lyophilization is often used as a process of choice in the pharmaceutical industry especially when labile compounds are used. The state of the art of lyophilization nanocapsules is reviewed. The formulation properties and the process parameters are discussed for a complete understanding of their impact on the stability and storage of the final dried product. To assess the quality of the dried product, various characterization methods are also discussed.

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Fluorescence Sensing of Nucleic Acid by Curcumin Encapsulated Poly(Ethylene Oxide)-Block-Poly(Propylene Oxide)-Block-Poly(Ethylene Oxide) Based Nanocapsules

Cited by 10 publications

References 33 publications

Fluorescent Nanosystems for Drug Tracking and Theranostics: Recent Applications in the Ocular Field

Fluorescent Nanosystems for Drug Tracking and Theranostics: Recent Applications in the Ocular Field

Curcumin‐embedded DBPC liposomes coated with chitosan layer as a fluorescence nanosensor for the selective detection of ribonucleic acid

Lyophilization of Nanocapsules: Instability Sources, Formulation and Process Parameters

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