Fluorescence sensor for organophosphorus pesticide detection based on the alkaline phosphatase-triggered reaction

Dong, Jingjiao; Yang, Haitang; Li, Ying; Liu, Anran; Wei, Wei; Liu, Songqin

doi:10.1016/j.aca.2020.07.048

Cited by 40 publications

(13 citation statements)

References 39 publications

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“…Their activity towards triesters which are mainly used as pesticides and chemical warfare agents is usually extremely low. It allows the use of these enzymes in the analysis of OPCs via competitive inhibition of reaction with usual substrates [63]. Nevertheless, active variants of ALP and ACP acting on triesters are often isolated from insect pests and plants that have evolved resistance to the pesticides used, including due to these enzymes.…”

Section: Alkaline and Acid Phosphatasesmentioning

confidence: 99%

Enzymes, Reacting with Organophosphorus Compounds as Detoxifiers: Diversity and Functions

Lyagin

Ефременко

2021

IJMS

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Organophosphorus compounds (OPCs) are able to interact with various biological targets in living organisms, including enzymes. The binding of OPCs to enzymes does not always lead to negative consequences for the body itself, since there are a lot of natural biocatalysts that can catalyze the chemical transformations of the OPCs via hydrolysis or oxidation/reduction and thereby provide their detoxification. Some of these enzymes, their structural differences and identity, mechanisms, and specificity of catalytic action are discussed in this work, including results of computational modeling. Phylogenetic analysis of these diverse enzymes was specially realized for this review to emphasize a great area for future development(s) and applications.

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Section: Alkaline and Acid Phosphatasesmentioning

confidence: 99%

Enzymes, Reacting with Organophosphorus Compounds as Detoxifiers: Diversity and Functions

Lyagin

Ефременко

2021

IJMS

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“…20,21 Alkaline phosphatase can cause a dephosphorylation reaction for nucleic acids, 22 proteins, 23 small molecules, and could be responsible for removal of the phosphate group of pesticides. 24 Methyl paraoxon pesticides have been widely used in agriculture and could cause serious health problems for humans even at very low concentrations. 25 To date, many methods such as colorimetric, 26 capillary electrophoresis, 27 electrochemiluminescence, 28 surface plasmon resonance, 29 and surface enhanced Raman scattering 30 have been used for enzymatic detection of methyl paraoxon using materials such as carbon quantum dots, 31,32 gold nanoclusters, 33,34 MnO 2 nanosheets, 35 and CdTe quantum dots.…”

Section: Introductionmentioning

confidence: 99%

Alkaline Phosphatase Immobilized CdTe/ZnS Quantum Dots for Dual-Purpose Fluorescent and Electrochemical Detection of Methyl Paraoxon

Dewangan

Korram

Karbhal

et al. 2022

Ind. Eng. Chem. Res.

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A facile paper-based fluorescence sensing and electrochemical behavior of CdTe/ZnS quantum dots (QDs) for detection of methyl paraoxon has been reported. The hydrolytic activity of alkaline phosphatase (ALP) for methyl paraoxon was observed to produce p-nitrophenol in the presence of QDs. The fluorescence quenching of CdTe/ZnS QDs by p-nitrophenol was measured as a quantitative signal for the detection of methyl paraoxon. A paper chip-based sensor has been developed by deposition of CdTe/ZnS QDs into cellulose paper. The significant increase in oxidation current in the cyclic voltammogram of methyl paraoxon solution in the presence of ALP is observed using a glassy carbon electrode (GCE) modified with CdTe/ZnS QDs. The linear relationship between oxidation and reduction peak currents against concentration of methyl paraoxon is utilized as a quantitative signal of electrochemical detection of methyl paraoxon. Under optimum conditions, both fluorescenceand paper-based methods showed a limit of detection (LOD) ≈ 0.65 nM whereas cyclic voltammetry provided a 2-fold higher LOD (1.72 nM) for sensing of methyl paraoxon. Therefore, the present method is a new insight for the rapid, convenient, and trace amount detection of methyl paraoxon.

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“…Various studies on the detection of chlorpyrifos by various methods have been reported, including gas chromatography (GC), 6 gas chromatography-mass spectrometry (GC-MS), 7,8 cyclic voltammetry (CV), [9][10][11] high-performance liquid chromatography (HPLC), high-performance liquid chromatography-ultravioletvisible spectroscopy (HPLC-UV-vis), 12 differential pulse voltammetry (DPV), 13,14 amperometry, 15 photoelectrochemistry (PEC), 16,17 surface-enhanced Raman spectroscopy, 18 and spec-trouorimetry. [19][20][21] However, some difficult problems remain to be solved, including complex sample preparation, expensive instruments, and the need for professional operation. Therefore, an efficient and rapid on-site detection method for the detection of chlorpyrifos is urgently needed for food and environmental safety.…”

Section: Introductionmentioning

confidence: 99%

Thioglycolic acid-modified AuNPs as a colorimetric sensor for the rapid determination of the pesticide chlorpyrifos

Zhang

et al. 2022

Anal. Methods

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Fluorescence sensor for organophosphorus pesticide detection based on the alkaline phosphatase-triggered reaction

Cited by 40 publications

References 39 publications

Enzymes, Reacting with Organophosphorus Compounds as Detoxifiers: Diversity and Functions

Enzymes, Reacting with Organophosphorus Compounds as Detoxifiers: Diversity and Functions

Alkaline Phosphatase Immobilized CdTe/ZnS Quantum Dots for Dual-Purpose Fluorescent and Electrochemical Detection of Methyl Paraoxon

Thioglycolic acid-modified AuNPs as a colorimetric sensor for the rapid determination of the pesticide chlorpyrifos

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