Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires

Verardo, Damiano; Liljedahl, Leena; Richter, Corinna; Agnarsson, Björn; Axelsson, Ulrika; Prinz, Christelle; Höök, Fredrik; Borrebaeck, Carl; Linke, Heiner

doi:10.3390/nano11010227

Cited by 10 publications

(18 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on optics modeling for optimal lightguiding, the nanowire platforms were designed with the aim to display arrays of vertical nanowires with a diameter of ∼110 nm, expected to provide good lightguiding at the chosen fluorophore wavelength, 29 and with a spacing of 1 μm or more, sufficient to avoid optical nanowire–nanowire overlap at the illumination wavelength of 650 nm, 26 enabling single-nanowire analysis. In addition, for biomolecular functionalization purposes, the nanowires were modified with a SiO 2 coating of thickness t c .…”

Section: Experimental Sectionmentioning

confidence: 99%

“… 29 Previous studies demonstrated the applicability of GaP nanowire arrays in single-molecule detection to study the diffusivity and concentration of membrane-bound proteins, 27 and they have been used for the detection of protein biomarkers, providing a 20-fold increase on sensitivity in contrast to conventional flat sensing surfaces. 26 In addition, vertically oriented GaP nanowires can be synthesized on GaP substrates by metal–organic vapor phase epitaxy (MOVPE), a nanoparticle-seeded, vapor–liquid–solid process that allows for optimization of fluorescence enhancement with much higher control over morphology, orientation, and positioning, as compared to solution-based methods used for instance for ZnO nanowires. However, MOVPE of GaP nanowires is not easily compatible with large-scale biosensing applications, for example, point-of-care diagnostics, because nanowire growth is a slow process and requires expensive GaP wafer substrates.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced Optical Biosensing by Aerotaxy Ga(As)P Nanowire Platforms Suitable for Scalable Production

Valderas-Gutiérrez

Davtyan

Sivakumar

et al. 2022

ACS Appl. Nano Mater.

Self Cite

View full text Add to dashboard Cite

Sensitive detection of low-abundance biomolecules is central for diagnostic applications. Semiconductor nanowires can be designed to enhance the fluorescence signal from surface-bound molecules, prospectively improving the limit of optical detection. However, to achieve the desired control of physical dimensions and material properties, one currently uses relatively expensive substrates and slow epitaxy techniques. An alternative approach is aerotaxy, a high-throughput and substrate-free production technique for high-quality semiconductor nanowires. Here, we compare the optical sensing performance of custom-grown aerotaxy-produced Ga(As)P nanowires vertically aligned on a polymer substrate to GaP nanowires batch-produced by epitaxy on GaP substrates. We find that signal enhancement by individual aerotaxy nanowires is comparable to that from epitaxy nanowires and present evidence of single-molecule detection. Platforms based on both types of nanowires show substantially higher normalized-to-blank signal intensity than planar glass surfaces, with the epitaxy platforms performing somewhat better, owing to a higher density of nanowires. With further optimization, aerotaxy nanowires thus offer a pathway to scalable, low-cost production of highly sensitive nanowire-based platforms for optical biosensing applications.

show abstract

Section: Experimental Sectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced Optical Biosensing by Aerotaxy Ga(As)P Nanowire Platforms Suitable for Scalable Production

Valderas-Gutiérrez

Davtyan

Sivakumar

et al. 2022

ACS Appl. Nano Mater.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Taking advantage of a fluorescence-based assay, Verardo et al developed a single chain fragment variable (scFv) antibody-based microarray platform consisting of SiO 2 -coated GaP nanowires [ 27 ]. Protein markers such as CEACAM-1, IL-5, and MCP-1were measured.…”

Section: Assay Miniaturizationmentioning

confidence: 99%

“…For example, for the MCP-1 protein, a concentration on the order of 100 pg/mL was found in serum, while for IL-5 it was on the order of 10 pg/mL. The prospect of a LOD below 1 pg/mL (i.e., ~10–100 fM) raised the sensitivity by 20-fold compared to conventional ELISA, making it a state-of-the-art immunoassay [ 27 ]. This method however did require additional steps and reagents to synthesize the nanowires and the array, making it inconvenient and less economical to use for wider applications.…”

Section: Assay Miniaturizationmentioning

confidence: 99%

See 1 more Smart Citation

Pushing the detection limits: strategies towards highly sensitive optical-based protein detection

Momenbeitollahi

Cloet

2021

Anal Bioanal Chem

View full text Add to dashboard Cite

Proteins are one of the main constituents of living cells. Studying the quantities of proteins under physiological and pathological conditions can give valuable insights into health status, since proteins are the functional molecules of life. To be able to detect and quantify low-abundance proteins in biofluids for applications such as early disease diagnostics, sensitive analytical techniques are desired. An example of this application is using proteins as biomarkers for detecting cancer or neurological diseases, which can provide early, lifesaving diagnoses. However, conventional methods for protein detection such as ELISA, mass spectrometry, and western blotting cannot offer enough sensitivity for certain applications. Recent advances in optical-based micro- and nano-biosensors have demonstrated promising results to detect proteins at low quantities down to the single-molecule level, shining lights on their capacities for ultrasensitive disease diagnosis and rare protein detection. However, to date, there is a lack of review articles synthesizing and comparing various optical micro- and nano-sensing methods of enhancing the limits of detections of the antibody-based protein assays. The purpose of this article is to critically review different strategies of improving assay sensitivity using miniaturized biosensors, such as assay miniaturization, improving antibody binding capacity, sample purification, and signal amplification. The pros and cons of different methods are compared, and the future perspectives of this research field are discussed.

show abstract

Selective Growth of GaP Crystals on CMOS-Compatible Si Nanotip Wafers by Gas Source Molecular Beam Epitaxy

Kafi,

Kang,

Golz

et al. 2024

Crystal Growth & Design

View full text Add to dashboard Cite

Gallium phosphide (GaP) is a III−V semiconductor with remarkable optoelectronic properties, and it has almost the same lattice constant as silicon (Si). However, to date, the monolithic and large-scale integration of GaP devices with silicon remains challenging. In this study, we present a nanoheteroepitaxy approach using gas-source molecular-beam epitaxy for selective growth of GaP islands on Si nanotips, which were fabricated using complementary metal−oxide semiconductor (CMOS) technology on a 200 mm n-type Si(001) wafer. Our results show that GaP islands with sizes on the order of hundreds of nanometers can be successfully grown on CMOS-compatible wafers. These islands exhibit a zinc-blende phase and possess optoelectronic properties similar to those of a high-quality epitaxial GaP layer. This result marks a notable advancement in the seamless integration of GaPbased devices with high scalability into Si nanotechnology and integrated optoelectronics.

show abstract

Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires

Cited by 10 publications

References 46 publications

Enhanced Optical Biosensing by Aerotaxy Ga(As)P Nanowire Platforms Suitable for Scalable Production

Enhanced Optical Biosensing by Aerotaxy Ga(As)P Nanowire Platforms Suitable for Scalable Production

Pushing the detection limits: strategies towards highly sensitive optical-based protein detection

Selective Growth of GaP Crystals on CMOS-Compatible Si Nanotip Wafers by Gas Source Molecular Beam Epitaxy

Contact Info

Product

Resources

About