Fluorescence Spectroelectrochemical Sensor for 1-Hydroxypyrene

Pinyayev, Tatyana S.; Seliskar, Carl J.; Heineman, William R.

doi:10.1021/ac101883a

Cited by 44 publications

(30 citation statements)

References 31 publications

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“…However, we could detect the peaks corresponding to PYDOH by changing FD setting (Ex/Em: 385/425 nm). This setting is according to the data from Pinyayev et al (2010). Also, by using MS, we could detect PYDOH (m/z 233) in samples after deconjugation.…”

Section: Identification Of Pyrene Metabolites Formed In Each Speciesmentioning

confidence: 99%

Phase-II conjugation ability for PAH metabolism in amphibians: Characteristics and inter-species differences

et al. 2011

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The present study examines amphibian metabolic activity-particularly conjugation -by analysis of pyrene (a four ring, polycyclic aromatic hydrocarbon) metabolites using high-performance liquid chromatography (HPLC) with fluorescence detector (FD), a mass spectrometry detector (MS) system and kinetic analysis of conjugation enzymes. Six amphibian species were exposed to pyrene (dissolved in water): African claw frog (Xenopus laevis); Tago's brown frog (Rana tagoi); Montane brown frog (Rana ornativentris); Wrinkled frog (Rana rugosa); Japanese newt (Cynops pyrrhogaster); and Clouded salamander (Hynobius nebulosus); plus one fish species, medaka (Oryzias latipes); and a fresh water snail (Clithon retropictus), and the resultant metabolites were collected. Identification of pyrene metabolites by HPLC and ion-trap MS system indicated that medaka mainly excreted pyrene-1-glucuronide (PYOG), whilst pyrene-1-sulfate (PYOS) was the main metabolite in all amphibian species. Pyrene metabolites in amphibians were different from those in invertebrate fresh water snails. Inter-species differences were also observed in pyrene metabolism among amphibians. Metabolite analysis showed that frogs relied more strongly on sulfate conjugation than did Japanese newts and clouded salamanders. Furthermore, urodelan amphibians, newts and salamanders, excreted glucose conjugates of pyrene that were not detected in the anuran amphibians. Kinetic analysis of conjugation by hepatic microsomes and cytosols indicated that differences in excreted metabolites reflected differences in enzymatic activities. Furthermore, pyrenediol (PYDOH) glucoside sulfate was detected in the Japanese newt sample. This novel metabolite has not been reported previously to this report, in which we have identified unique characteristics of amphibians in phase II pyrene metabolism.

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Section: Identification Of Pyrene Metabolites Formed In Each Speciesmentioning

confidence: 99%

Phase-II conjugation ability for PAH metabolism in amphibians: Characteristics and inter-species differences

et al. 2011

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“…Ru(bpy) 3 2 + is a model system for spectroelectrochemical sensing because it uptakes into the film and its redox couple is within the working range of many thin film electrodes. The use of SSEBS and Nafion coated OTEs has been extensively studied by our group [22][23][24][25]. Figure 5A shows cyclic voltammograms of Ru(bpy) 3 2 + both on bare BDD and BDD coated with a 210 nm Nafion film.…”

Section: Spectroelectrochemistrymentioning

confidence: 99%

“…This is demonstrated with tris(2,2'-bipyridyl)ruthenium(II) ion [Ru(bpy) 3 2 + ] using an attenuated total reflectance (ATR) flow cell setup for both absorption and fluorescence. With a Nafion coated BDD optically transparent thin layer electrode (OTTLE), we developed a fluorescence based sensor for a common polyWhile they play an important role in dye, plastic, and pesticide production, polycyclic aromatic hydrocarbons (PAHs) are harmful environmental contaminants [25]. This contamination comes from a vast number of sources, both natural and man-made.…”

Section: Introductionmentioning

confidence: 99%

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Polymer‐coated Boron Doped Diamond Optically Transparent Electrodes for Spectroelectrochemical Sensors

et al. 2016

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Spectroelectrochemical sensors combine electrochemistry, spectroscopy, and partitioning into a film to provide improved selectivity for the target analyte. The sensor usually consists of an optically transparent electrode (OTE) coated with a charge selective polymer film. The polymer film is chosen to pre-concentrate analyte at the OTE surface to improve the sensitivity and provide selectivity against like charged interferences. OTEs such as Indium Tin Oxide (ITO) have been used extensively for spectroelectrochemical sensors, but little is known about the applicability of such sensors using other OTE materials, such as Boron Doped Diamond (BDD). One distinct advantage of BDD OTEs over ITO OTEs is their significant increase in sensitivity for organic compounds, such as 4-aminophenol and hydroquinone. We have developed absorption and fluorescence-based sensing methods with a BDD OTE coated with a sulfonated ionomer film, Nafion

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“…53 1-PyOH was oxidized under acidic conditions to diquinonepyrene. Diquinonepyrene has reversible electrochemistry and fluoresces at 425 nm in its reduced form, dihydroxypyrene.…”

Section: Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 99%

Spectroelectrochemistry as a strategy for improving selectivity of sensors for security and defense applications

Heineman¹,

Seliskar²,

Morris³

et al. 2012

SPIE Proceedings

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Spectroelectrochemistry provides improved selectivity for sensors by electrochemically modulating the optical signal associated with the analyte. The sensor consists of an optically transparent electrode (OTE) coated with a film that preconcentrates the target analyte. The OTE functions as an optical waveguide for attenuated total reflectance (ATR) spectroscopy, which detects the analyte by absorption. Alternatively, the OTE can serve as the excitation light for fluorescence detection, which is generally more sensitive than absorption. The analyte partitions into the film, undergoes an electrochemical redox reaction at the OTE surface, and absorbs or emits light in its oxidized or reduced state. The change in the optical response associated with electrochemical oxidation or reduction at the OTE is used to quantify the analyte. Absorption sensors for metal ion complexes such as [Fe(CN) 6 ] 4-and [Ru(bpy) 3 ] 2+ and fluorescence sensors for [Ru(bpy) 3 ] 2+ and the polycyclic aromatic hydrocarbon 1-hydroxypyrene have been developed. The sensor concept has been extended to binding assays for a protein using avidin-biotin and 17β-estradiol-anti-estradiol antibodies. The sensor has been demonstrated to measure metal complexes in complex samples such as nuclear waste and natural water. This sensor has qualities needed for security and defense applications that require a high level of selectivity and good detection limits for target analytes in complex samples. Quickly monitoring and designating intent of a nuclear program by measuring the Ru/Tc fission product ratio is such an application.

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Fluorescence Spectroelectrochemical Sensor for 1-Hydroxypyrene

Cited by 44 publications

References 31 publications

Phase-II conjugation ability for PAH metabolism in amphibians: Characteristics and inter-species differences

Phase-II conjugation ability for PAH metabolism in amphibians: Characteristics and inter-species differences

Polymer‐coated Boron Doped Diamond Optically Transparent Electrodes for Spectroelectrochemical Sensors

Spectroelectrochemistry as a strategy for improving selectivity of sensors for security and defense applications

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