Fluorescence studies of the intra-cellular distribution of zinc bis(thiosemicarbazone) complexes in human cancer cells

Abstract

The uptake of zinc bis(thiosemicarbazone) complexes in human cancer cells has been studied by fluorescence microscopy and the cellular distribution established, including the degree of uptake in the nucleus.

In Vitro and In Vivo Evaluations of a Hydrophilic 64Cu-Bis(Thiosemicarbazonato)–Glucose Conjugate for Hypoxia Imaging

“…The proligand H 2 -ATSM, Zn-ATSM, and Zn-ATSE/A-G were prepared according to published methods (17)(18)(19)(20).…”

EuroTracker dyes: highly emissive europium complexes as alternative organelle stains for live cell imaging

“…Recently, we introduced a new class of highly emissive europium complexes, with extinction coefficients in the range 55-60,000 M -1 cm -1 (330 to 350 nm) and an emission quantum yield in methanol of around 50%. 27 Due to their high 10 brightness, these systems show promise as intracellular stains, allowing rapid spectral imaging acquisition using CCD detectors.…”

“…Over the last decade, emissive metal coordination complexes have emerged as a distinctive class of intracellular stains and probes. [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] Complexes of the lanthanide(III) ions offer particular scope in this respect, [17][18][19][20][21][22][23][24][25][26] minimizes self-quenching and an optical signal that conveys information about the local environment through changes in emission spectral form and lifetime. A key feature of luminescent lanthanide(III) complexes is the longer lifetime of emission; this permits the use of time-gated detection methods to eliminate 5 short-lived autofluorescence from biomolecules, generating high quality images.…”

Selective Intracellular Release of Copper and Zinc Ions from Bis(thiosemicarbazonato) Complexes Reduces Levels of Alzheimer Disease Amyloid-β Peptide

“…btsc complexes are also capable of transporting Zn II into cells, and the intrinsic fluorescence of certain Zn II (btsc) complexes has been used to probe their intracellular distribution in several lines of cancer cells (27). Zn II , like Cu, is central to a number of cell signal pathways including modulation of N-methyl-D-aspartate receptor activity (28), expression of metallothioneins (29,30) and activation of MAPK-mediated signal transduction pathways (31).…”