There is a need for an inexpensive, reliable and fast monitoring tool to detect contaminants in a short time, for quick mitigation of pollution sources and site remediation, and for characterisation of natural dissolved organic matter (DOM). Fluorescence spectroscopy has proven to be an excellent technique in quantifying aquatic DOM, from autochthonous, allochthonous or anthropogenic sources. This paper reviews the advances in in situ fluorescence measurements of DOM and pollutants in various water environments. Studies have demonstrated, using high temporal-frequency DOM fluorescence data, that marine autochthonous production of DOM is highly complex and that the allochthonous input of DOM from freshwater to marine water can be predicted. Furthermore, river measurement studies found a delayed fluorescence response of DOM following precipitation compared to turbidity and discharge, with various lags, depending on season, site and input of dissolved organic carbon (DOC) concentration. In addition, research has shown that blue light fluorescence (emission = 430-500 nm) can be a good proxy for DOC, in environments with terrestrial inputs, and ultraviolet fluorescence (emission = UVA-320-400 nm) for biochemical oxygen demand, and also E. coli in environments with sanitation issues. The correction of raw fluorescence data improves the relationship between fluorescence intensity and these parameters. This review also presents the specific steps and parameters that must be considered before and 2 during in situ fluorescence measurement session for a harmonised qualitative and quantitative protocol. Finally, the strengths and weaknesses of the research on in situ fluorescence are identified.