Fluorescence Tomography of Biological Tissue Based on Ultrasound Tagging Technique

Kobayashi, Masaki; Mizumoto, Takashi; Duc, Trinh Quang; Takeda, Motohiro

doi:10.1364/ecbo.2007.6633_5

Biophotonics 2007: Optics in Life Science 2007

DOI: 10.1364/ecbo.2007.6633_5

|View full text |Cite

Fluorescence Tomography of Biological Tissue Based on Ultrasound Tagging Technique

Masaki Kobayashi

Takashi Mizumoto

Trinh Quang Duc

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Publication Types

Select...

Book1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By exploiting the spectral separation between fluorescence and excitation using an appropriate optical arrangement with a large-scale, highly efficient detector, an ultrasound field offers a detectable modulation of the fluorescence signal. 8,9 For this demonstration study, we show tomographic imaging of fluorescence in dense scattering media using porcine muscle…”

mentioning

confidence: 99%

Fluorescence Tomography

Encyclopedia of Diagnostic Imaging

View full text Add to dashboard Cite

Ultrasound-induced modulation permits the collection of fluorescence images from deep within light-scattering media.Fluorescence labeling traces specific molecules that play physiological roles in living organisms, revealing the mysteries of life. Recently developed fluorescent probes with excellent optical performance are expected to extend fluorescence techniques to clinical diagnosis. However, living tissues scatter light strongly, restricting applications to the microscopic level or to the body surface. To permit noninvasive optical measurement for tomographic imaging of the human body, we have explored a technique to visualize fluorescence at a depth of tens of centimeters, with resolution of the order of millimeters.Myriad efforts have been made over many years to develop fluorescence and other optical imaging techniques that work in light-scattering media. Time-and frequency-domain techniques 1 for analyzing diffuse light and the extraction of forward-scattering light by coherent gating 2, 3 are the major approaches to optical tomography. The distribution of optical absorption has been measured using 'ultrasonic tagging,' which focuses more weakly scattered ultrasound to extract spatial information about optical properties. [4][5][6][7] We have extended focused ultrasound to visualize fluorescence in dense scattering media.Previously, ultrasonic tagging was thought to be unsuitable for fluorescence imaging because speckle modulation in multiply-scattering media occurs only for coherent optical processes. However, even for fluorescence, density variation of the medium in a focused sound field might induce intensity modulation. By exploiting the spectral separation between fluorescence and excitation using an appropriate optical arrangement with a large-scale, highly efficient detector, an ultrasound field offers a detectable modulation of the fluorescence signal. 8,9 For this demonstration study, we show tomographic imaging of fluorescence in dense scattering media using porcine muscle

show abstract

mentioning

confidence: 99%

Fluorescence Tomography

Encyclopedia of Diagnostic Imaging

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Fluorescence Tomography of Biological Tissue Based on Ultrasound Tagging Technique

Cited by 1 publication

References 0 publications

Fluorescence Tomography

Fluorescence Tomography

Contact Info

Product

Resources

About