Fluorescence-tunable Ag-DNA biosensor with tailored cytotoxicity for live-cell applications

Bossert, Nelli; Bruin, Donny de; Götz, Margarete; Bouwmeester, Dirk; Heinrich, Doris

doi:10.1038/srep37897

Cited by 17 publications

(10 citation statements)

References 51 publications

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“…Not only inorganic analytes can be sensed with silver nanoclusters, but also the detection of biomolecules has been explored by several groups. Among examples of biomolecular detection are gene detection [23], protein detection [24][25][26], detection of specific nucleic acid sequences [27][28][29], and cellular labeling [30,31]. Recently, advanced detection procedures have also been incorporated with the use of AgNCs such as the multiplexed sensing capability of several genes [32] or surface plasmon enhanced energy transfer (SPEET) between AgNCs and AuNP for protein [33] or DNA [34] detection.…”

Section: Introductionmentioning

confidence: 99%

Micro RNA Sensing with Green Emitting Silver Nanoclusters

Yourston

Krasnoslobodtsev

2020

Molecules

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Micro RNA (miR) are regulatory non-coding RNA molecules, which contain a small number of nucleotides ~18–28 nt. There are many various miR sequences found in plants and animals that perform important functions in developmental, metabolic, and disease processes. miRs can bind to complementary sequences within mRNA molecules thus silencing mRNA. Other functions include cardiovascular and neural development, stem cell differentiation, apoptosis, and tumors. In tumors, some miRs can function as oncogenes, others as tumor suppressors. Levels of certain miR molecules reflect cellular events, both normal and pathological. Therefore, miR molecules can be used as biomarkers for disease diagnosis and prognosis. One of these promising molecules is miR-21, which can serve as a biomarker with high potential for early diagnosis of various types of cancer. Here, we present a novel design of miR detection and demonstrate its efficacy on miR-21. The design employs emissive properties of DNA-silver nanoclusters (DNA/AgNC). The detection probe is designed as a hairpin DNA structure with one side of the stem complimentary to miR molecule. The binding of target miR-21 opens the hairpin structure, dramatically modulating emissive properties of AgNC hosted by the C12 loop of the hairpin. “Red” fluorescence of the DNA/AgNC probe is diminished in the presence of the target miR. At the same time, “green” fluorescence is activated and its intensity increases several-fold. The increase in intensity of “green” fluorescence is strong enough to detect the presence of miR-21. The intensity change follows the concentration dependence of the target miR present in a sample, which provides the basis of developing a new, simple probe for miR detection. The detection strategy is specific, as demonstrated using the response of the DNA/AgNC probe towards the scrambled miR-21 sequence and miR-25 molecule. Additionally, the design reported here is very sensitive with an estimated detection limit at ~1 picomole of miR-21.

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Section: Introductionmentioning

confidence: 99%

Micro RNA Sensing with Green Emitting Silver Nanoclusters

Yourston

Krasnoslobodtsev

2020

Molecules

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“…[18] Polymersomes that are slowly degraded by the cell are useful for studying the uptake and trafficking of nanoparticles in the endo-lysosomal pathways. [19][20][21][22] The potential of polymerosomes for bio-imaging and drug delivery has been investigated, [3,9,10,12,18,[23][24][25][26][27][28][29][30][31] but very few studies have paid attention to their uptake dynamics, long-term imaging, and breakdown in vitro and in vivo, although these parameters are critical to conclude on the usability of polymersomes for biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of dual-fluorescent polymersomes with durable integrity in living cancer cells and zebrafish embryos

et al. 2018

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The long-term fate of biomedical nanoparticles after endocytosis is often only sparsely addressed in vitro and in vivo, while this is a crucial parameter to conclude on their utility. In this study, dual-fluorescent polyisobutylene-polyethylene glycol (PiB-PEG) polymersomes were studied for several days in vitro and in vivo. In order to optically track the vesicles' integrity, one fluorescent probe was located in the membrane and the other in the aqueous interior compartment. These non-toxic nanovesicles were quickly endocytosed in living A549 lung carcinoma cells but unusually slowly transported to perinuclear lysosomal compartments, where they remained intact and luminescent for at least 90 h without being exocytosed. Fluorescence-assisted flow cytometry indicated that after endocytosis, the nanovesicles were eventually degraded within 7-11 days. In zebrafish embryos, the polymersomes caused no lethality and were quickly taken up by the endothelial cells, where they remained fully intact for as long as 96 h post-injection. This work represents a novel case-study of the remarkable potential of PiB-PEG polymersomes as an in vivo bio-imaging and slow drug delivery platform.

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“…Enhanced secretion of adipose-derived hormones termed adipokines or adipocytokines, like growth factors and pro-inflammatory cytokines, allows to focus on elements for studies of the pathogenesis of tumor growth, increased mobility and cell migration, and subsequently cancer metastasis [1, 2]. The adipokines could play a significant role in regulation of cell growth, proliferation, cell cycle, angiogenesis and also tumor growth and metastases [3].…”

Section: Introductionmentioning

confidence: 99%

The adipokine vaspin reduces apoptosis in human hepatocellular carcinoma (Hep-3B) cells, associated with lower levels of NO and superoxide anion

Skonieczna

Hudy

Hejmo

et al. 2019

BMC Pharmacol Toxicol

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Background Among adipose-derived factors, adipocytokines play roles as hormones and signaling mediators for apoptotic pathway. Among of them, vaspin, regulates the metabolism of adipose tissue itself as an endocrine organ, and stimulates adipocytes to maturation, differentiation, etc. Damaged adipocytes, present in obesity and hepatocellular carcinoma (HCC) respond with over-production of inflammatory cytokines. Such pro-inflammatory stimulation remains under adipokine control. Pro-inflammatory pathways are connected to oxidative stress and apoptosis, reported as co-existing with an elevated level of some adipokines in cancer cell lines. However, some hormones, such as vaspin, reduce apoptosis, have anti-inflammatory and anti-oxidative roles in cancer cell lines. Methods Hep-3B cells were cytometrically evaluated under vaspin treatment for reactive oxygen species (ROS) and apoptosiss induction. The statistical significant changes to the untreated controls was calculated by T-tests (indicated at value p < 0.05). Results Here we studied the production of reactive oxygen and nitrogen species in cells of HCC line Hep-3B after vaspin treatment. A decreased level of nitric oxide and superoxide anion 24 h after vaspin addition at 5 ng/ml was correlated with restricted, to the physiological level, apoptosis. A protective role of vaspin was displayed as enhanced cell viability and proliferation, which could be a poor prognostic in liver cancer. Conclusions Apoptosis was suppressed after vaspin treatment, together with low levels of nitric oxide and superoxide anions.

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Fluorescence-tunable Ag-DNA biosensor with tailored cytotoxicity for live-cell applications

Cited by 17 publications

References 51 publications

Micro RNA Sensing with Green Emitting Silver Nanoclusters

Micro RNA Sensing with Green Emitting Silver Nanoclusters

Dynamics of dual-fluorescent polymersomes with durable integrity in living cancer cells and zebrafish embryos

The adipokine vaspin reduces apoptosis in human hepatocellular carcinoma (Hep-3B) cells, associated with lower levels of NO and superoxide anion

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