Fluorescence turn-on sensing of protein based on mannose functionalized perylene bisimides and its fluorescence imaging

Wang, Ke-Rang; An, Hong‐Wei; Rong, Rui-Xue; Cao, Ziping; Li, Xiaoliu

doi:10.1016/j.bios.2014.02.038

Cited by 25 publications

(15 citation statements)

References 63 publications

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“…We next wanted to assess CD206-mediated endocytic uptake in our macrophages. This was determined using PBI-12-Man, a biocompatible agent that displays fluorescence following binding to mannose receptor [17]. After a sixty-minute treatment of both M1 and M2 macrophages with PBI-12-Man, the red fluorescence of PBI-12-Man was predominantly intracellular in M2 macrophages under phased and continuous treatment conditions (Figure 4A–B).…”

Section: Resultsmentioning

confidence: 99%

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A Phased Strategy to Differentiate Human CD14 ⁺ Monocytes into Classically and Alternatively Activated Macrophages and Dendritic Cells

et al. 2016

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There are currently several in vitro strategies to differentiate human CD14+ monocytes isolated from peripheral blood mononuclear cells into M1 or M2 macrophage cell types. Thereafter, each cell type is then verified using flow cytometric analysis of cell surface markers. Human CD14+ monocytes have the potential to differentiate into M1 and M2 macrophages, both of which demonstrate varying degrees of cell surface antigen overlap. Using multiple surface markers with current macrophage polarization protocols, our data reveal several limitations with current methods, such as highly ambiguous cell types that possess cell surface marker overlap and functional similarities. By utilizing interleukin-6 and two phases of cytokine exposure, we have developed a protocol to differentiate human monocytes into M1, M2, or dendritic cells with a greater efficiently and fidelity relative to macrophages and dendritic cells that are produced from commonly used methods. This is achieved via alterations in cytokine composition, dosing, incubation times, and improvements in verification methodology. This report provides a reliable method to reproduce human in vitro monocyte derived dendritic cells and macrophage models that will aid in defining and understanding innate and adaptive immunity better as well as several pathologic states.

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Section: Resultsmentioning

confidence: 99%

“…Perylene bisimide (PBI-12-Man) [17] was a kind gift from Dr. Ke-Rang Wang from Hebei University. Cells were then cultured with 10 μg/mL PBI-12-Man for 30 minutes at 37°C then washed with 1X PBS.…”

Section: Methodsmentioning

confidence: 99%

A Phased Strategy to Differentiate Human CD14 ⁺ Monocytes into Classically and Alternatively Activated Macrophages and Dendritic Cells

et al. 2016

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“…Further in vivo experiments confirmed their selective imaging capability . Perylene bis(imide) derivative decorated with mannose was found to be fluorescent only after the degradation of the formed scaffold triggered by its ligation to a mannose‐specific lectin . The fluorescent imaging of macrophage cells overexpressing such receptors has been reported (Fig.…”

Section: Cell/tissue Targetingmentioning

confidence: 62%

“…The fluorescent imaging of macrophage cells overexpressing such receptors has been reported (Fig. ) . A novel NIR fluorophore activated upon isomerization at a low pH in lysosomes was used by Wu et al Biocompatible poly(styrene‐co‐maleic acid) NPs loaded with this probe specifically targeting surface‐displayed sialic acid were able to selectively tag and mark tumors in vivo .…”

Section: Cell/tissue Targetingmentioning

confidence: 99%

“…For the inhibition experiment, in the presence of α ‐d‐mannopyranoside (d–f), the remarkable decrease of the red fluorescence of PBI‐12‐Man indicated the selectively binding interactions of PBI‐12‐Man with the surface mannose receptor of the macrophage cells. Reprinted from . Copyright 2014, with permission from Elsevier.…”

Section: Cell/tissue Targetingmentioning

confidence: 99%

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Nanotechnology in Glycomics: Applications in Diagnostics, Therapy, Imaging, and Separation Processes

Dosekova

Filip

Bertók

et al. 2016

Medicinal Research Reviews

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This review comprehensively covers the most recent achievements (from 2013) in the successful integration of nanomaterials in the field of glycomics. The first part of the paper addresses the beneficial properties of nanomaterials for the construction of biosensors, bioanalytical devices, and protocols for the detection of various analytes, including viruses and whole cells, together with their key characteristics. The second part of the review focuses on the application of nanomaterials integrated with glycans for various biomedical applications, that is, vaccines against viral and bacterial infections and cancer cells, as therapeutic agents, for in vivo imaging and nuclear magnetic resonance imaging, and for selective drug delivery. The final part of the review describes various ways in which glycan enrichment can be effectively done using nanomaterials, molecularly imprinted polymers with polymer thickness controlled at the nanoscale, with a subsequent analysis of glycans by mass spectrometry. A short section describing an active glycoprofiling by microengines (microrockets) is covered as well.

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Unique SiO₂ Nanourchins Enable Amplification in Living Cells for In Situ Imaging of mRNAs

Shen

Wang

Zhang

et al. 2018

Adv Funct Materials

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Sufficient delivery of nucleic acid reagents into cells for amplifications with great activities would be significant for in situ intracellular imaging of messenger RNAs in living cells, showing great potentials in chemistry and biology. Herein, SiO 2 nanourchins (SNUs) are developed, with two-pronged pair of primers anchored on enzyme-functionalized mesoporous SiO 2 cores, for in situ imaging of mRNAs in living cells. Endowed by the urchinslike structure to represent high loading capacity, efficient activity without mutual interference, and sufficient delivery into cells, SNUs maintain sustainable reactivities for amplification through primers' 3′-end complete exposure and synergistic reagents release by increased dissociation or mobility during hybridization. "One-step" reverse-transcription helicasedependent isothermal amplifications are employed in living cells with Klenow (exo − ) DNA polymerase to accomplish reverse transcriptions and polymerization amplifications. With high efficiency and stability, low toxicity, and good specificity, this method can detect amol of mRNA in dozens of microliter samples (<0.02 molecules per cell) in living cells, providing an ultrasensitive tool for fundamental understanding of mRNAs in cellular processes.

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Fluorescence turn-on sensing of protein based on mannose functionalized perylene bisimides and its fluorescence imaging

Cited by 25 publications

References 63 publications

A Phased Strategy to Differentiate Human CD14 ⁺ Monocytes into Classically and Alternatively Activated Macrophages and Dendritic Cells

A Phased Strategy to Differentiate Human CD14 ⁺ Monocytes into Classically and Alternatively Activated Macrophages and Dendritic Cells

Nanotechnology in Glycomics: Applications in Diagnostics, Therapy, Imaging, and Separation Processes

Unique SiO₂ Nanourchins Enable Amplification in Living Cells for In Situ Imaging of mRNAs

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Fluorescence turn-on sensing of protein based on mannose functionalized perylene bisimides and its fluorescence imaging

Cited by 25 publications

References 63 publications

A Phased Strategy to Differentiate Human CD14 + Monocytes into Classically and Alternatively Activated Macrophages and Dendritic Cells

A Phased Strategy to Differentiate Human CD14 + Monocytes into Classically and Alternatively Activated Macrophages and Dendritic Cells

Nanotechnology in Glycomics: Applications in Diagnostics, Therapy, Imaging, and Separation Processes

Unique SiO2 Nanourchins Enable Amplification in Living Cells for In Situ Imaging of mRNAs

Contact Info

Product

Resources

About

A Phased Strategy to Differentiate Human CD14 ⁺ Monocytes into Classically and Alternatively Activated Macrophages and Dendritic Cells

A Phased Strategy to Differentiate Human CD14 ⁺ Monocytes into Classically and Alternatively Activated Macrophages and Dendritic Cells

Unique SiO₂ Nanourchins Enable Amplification in Living Cells for In Situ Imaging of mRNAs