Fluorescent aptamer-modified mesoporous silica nanoparticles for quantitative acetamiprid detection

He, Kangli; Yang, Jinghan; Shi, Qiuyun; Guan, Lingjun; Sun, Li; Chen, Ziyang; Feng, Jianguo; Dong, Sa

doi:10.1007/s11356-022-21970-9

Cited by 7 publications

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“…Finally, XRD was used to verify whether carboxylation can affect the structure of the SiO 2 nanoparticles. As shown in Figure F, there is a wide hummock-shaped peak at 2θ = 22°, which is a typical peak for SiO 2 . There is no difference between the XRD pattern of SiO 2 –COOH and that of SiO 2 , indicating that carboxylation has no effect on the structure of SiO 2 .…”

Section: Resultsmentioning

confidence: 91%

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Fluorescence and Colorimetric Dual-Signal Aptamer Sensor Based on Aptamer-Functionalized Fe₃O₄ Nanoparticles Decorated with Au and Pt Nanoparticles Combined with Fluorescently Labeled Aptamer Complementary Chain-Functionalized SiO₂ Nanoparticles for Detecting the Insecticide Acetamiprid

Dong,

Zhu,

Shi

et al. 2023

ACS Appl. Nano Mater.

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Acetamiprid (ACE) is a nicotinic chloride insecticide with certain genotoxicity and cytotoxicity that poses a threat to human health. Therefore, it is necessary to establish a rapid and sensitive method for the detection of ACE in agricultural products. In this study, Au and Pt nanoparticles with enzyme-catalyzing and fluorescence-quenching ability were coated on the surface of Fe 3 O 4 nanoparticles to obtain Fe 3 O 4 −Au/Pt. Simultaneously, SiO 2 nanoparticles were synthesized. Aptamer (Apt)-functionalized Fe 3 O 4 −Au/Pt (Fe 3 O 4 −Au/Pt-Apt) and fluorescently labeled aptamers with complementary chain-functionalized SiO 2 (SiO 2 -cDNA-FAM) were used as identification probes. Based on the specific recognition of Apts to ACE, the enzymatic activity of Fe 3 O 4 −Au/Pt, and its fluorescence quenching effect, combined with magnetic separation, a fluorescence and colorimetric dual-signal aptamer sensor for ACE detection was constructed. Based on the optimal conditions, ACE in the concentration of 0.5−6 μg/mL presents a good linear correlation with ΔF (ΔF = F − F 0 , F and F 0 are fluorescence intensities in the presence or absence of ACE) and absorbance value (A). The standard curve of the fluorescence signal was ΔF = 233.68C + 34.48. R 2 = 0.9937, and the limit of detection (LOD) was 0.42 μg/mL. The standard curve of the colorimetric signal was A = 0.0385C + 0.0486, with R 2 = 0.9966, and the LOD was 0.39 μg/mL. The method had no cross-reaction with thiamethoxam (THI), nitenpyram (NIT), imidacloprid (IMI), and thiamethoxam (CLO). The average recoveries of ACE in actual samples (Chinese cabbage and cucumber) were 92.69%− 102.54% (CV < 3.67%) and 85.59%−97.52% (CV < 3.54%) for fluorescence and colorimetric signals, respectively. The above results show that the sensor has good specificity and can be used for actual sample detection. Meanwhile, this study provides technical support for pesticide residue detection.

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Section: Resultsmentioning

confidence: 91%

“…As shown in Figure 1F, there is a wide hummock-shaped peak at 2θ = 22°, which is a typical peak for SiO 2 . 34 There is no difference between the XRD pattern of SiO 2 −COOH and that of SiO 2 , indicating that carboxylation has no effect on the structure of SiO 2 . on Fe 3 O 4 particles.…”

Section: Recovery Test In Actual Samplesmentioning

confidence: 94%

Fluorescence and Colorimetric Dual-Signal Aptamer Sensor Based on Aptamer-Functionalized Fe₃O₄ Nanoparticles Decorated with Au and Pt Nanoparticles Combined with Fluorescently Labeled Aptamer Complementary Chain-Functionalized SiO₂ Nanoparticles for Detecting the Insecticide Acetamiprid

Dong,

Zhu,

Shi

et al. 2023

ACS Appl. Nano Mater.

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Unlocking the potential of mesoporous silica nanoparticles in breast cancer treatment

Thapa,

Ali,

Afzal

et al. 2023

J Nanopart Res

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An innovative biosensor utilizing aptamer-gated mesoporous silica nanoparticles for determination of aminoglycoside antibiotics through indirect-lateral flow

Ghaffari,

Moradi,

Danesh

et al. 2025

Analytica Chimica Acta

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Fluorescent aptamer-modified mesoporous silica nanoparticles for quantitative acetamiprid detection

Cited by 7 publications

References 50 publications

Fluorescence and Colorimetric Dual-Signal Aptamer Sensor Based on Aptamer-Functionalized Fe₃O₄ Nanoparticles Decorated with Au and Pt Nanoparticles Combined with Fluorescently Labeled Aptamer Complementary Chain-Functionalized SiO₂ Nanoparticles for Detecting the Insecticide Acetamiprid

Fluorescence and Colorimetric Dual-Signal Aptamer Sensor Based on Aptamer-Functionalized Fe₃O₄ Nanoparticles Decorated with Au and Pt Nanoparticles Combined with Fluorescently Labeled Aptamer Complementary Chain-Functionalized SiO₂ Nanoparticles for Detecting the Insecticide Acetamiprid

Unlocking the potential of mesoporous silica nanoparticles in breast cancer treatment

An innovative biosensor utilizing aptamer-gated mesoporous silica nanoparticles for determination of aminoglycoside antibiotics through indirect-lateral flow

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Fluorescent aptamer-modified mesoporous silica nanoparticles for quantitative acetamiprid detection

Cited by 7 publications

References 50 publications

Fluorescence and Colorimetric Dual-Signal Aptamer Sensor Based on Aptamer-Functionalized Fe3O4 Nanoparticles Decorated with Au and Pt Nanoparticles Combined with Fluorescently Labeled Aptamer Complementary Chain-Functionalized SiO2 Nanoparticles for Detecting the Insecticide Acetamiprid

Fluorescence and Colorimetric Dual-Signal Aptamer Sensor Based on Aptamer-Functionalized Fe3O4 Nanoparticles Decorated with Au and Pt Nanoparticles Combined with Fluorescently Labeled Aptamer Complementary Chain-Functionalized SiO2 Nanoparticles for Detecting the Insecticide Acetamiprid

Unlocking the potential of mesoporous silica nanoparticles in breast cancer treatment

An innovative biosensor utilizing aptamer-gated mesoporous silica nanoparticles for determination of aminoglycoside antibiotics through indirect-lateral flow

Contact Info

Product

Resources

About

Fluorescence and Colorimetric Dual-Signal Aptamer Sensor Based on Aptamer-Functionalized Fe₃O₄ Nanoparticles Decorated with Au and Pt Nanoparticles Combined with Fluorescently Labeled Aptamer Complementary Chain-Functionalized SiO₂ Nanoparticles for Detecting the Insecticide Acetamiprid

Fluorescence and Colorimetric Dual-Signal Aptamer Sensor Based on Aptamer-Functionalized Fe₃O₄ Nanoparticles Decorated with Au and Pt Nanoparticles Combined with Fluorescently Labeled Aptamer Complementary Chain-Functionalized SiO₂ Nanoparticles for Detecting the Insecticide Acetamiprid