Fluorescent banding and meiotic behaviour in Erythrodiplax nigricans (Libellulidae) and Coryphaeschna perrensi (Aeschnidae) (Anisoptera, Odonata)

Diego, De Gennaro; Rebagliati, Pablo Javier; Mola, Liliana M.

doi:10.1080/00087114.2008.10589610

Cited by 8 publications

(5 citation statements)

References 14 publications

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“…The source of difficulties in identifying these chromosomes is often the fact that the size of the smallest chromosome pair can vary between both different species and even different populations of the same species (Kiauta, 1968b; Mola, 2007). Furthermore, some authors have shown that m‐chromosomes may be present in some populations and absent in other populations of the same species (e.g., Agopian & Mola, 1988; Diego et al., 2008; Kiauta, 1969a, 1969b), and this may be the case also with the species studied by us. In this context, noteworthy is a male of S. flavomaculata in which several cells had an additional minute chromosome suggesting a chromosomal mosaicism caused by an extra m‐chromosome.…”

Section: Discussionsupporting

confidence: 69%

Chromosomal analysis of eight species of dragonflies (Anisoptera) and damselflies (Zygoptera) using conventional cytogenetics and fluorescence in situ hybridization: Insights into the karyotype evolution of the ancient insect order Odonata

Kuznetsova

Maryańska-Nadachowska

Anokhin

et al. 2020

J. Zool. Syst. Evol. Res.

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All Odonata species studied to date using fluorescence in situ hybridization (FISH) belong to the dragonfly (Anisoptera) families Corduliidae and Libellulidae. It was shown that 18S rRNA gene loci locate on one of the largest pairs of autosomes in every species, whereas the “insect” telomere motif (TTAGG)n is absent in all but one species. For better understanding the chromosomal organization and evolution of Odonata, we used C‐banding and FISH to study the karyotypes and map TTAGG sequences and major rRNA loci on chromosomes of three more dragonfly species from the families Corduliidae, Libellulidae, and Aeshnidae. Moreover, we obtained the first FISH‐data on the suborder Zygoptera (damselflies) by analyzing five species of the families Coenagrionidae and Calopterygidae. We showed that all studied dragonfly species had 2n = 24A + X. The same karyotype was observed in the damselfly family Coenagrionidae, whereas in species of the Calopterygidae, the karyotype 2n = 26A + X was found. Both dragonfly and damselfly species had a pair of m‐chromosomes; constitutive heterochromatin tended to be concentrated in the terminal regions of their chromosomes. The use of (TTAGG)n and 18S rRNA gene probes in dual‐color FISH did not generate (TTAGG)n fluorescent signals in any species; major rRNA clusters were revealed on one of the largest pairs of autosomes in all Anisoptera species but on m‐chromosomes in all Zygoptera species. Our results suggest that the former 18S location pattern was ancestral in the Odonata and the latter pattern had an ancient origin and could arise in a common ancestor of the damselfly superfamilies Calopterygoidea and Coenagrionoidea.

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Section: Discussionsupporting

confidence: 69%

Chromosomal analysis of eight species of dragonflies (Anisoptera) and damselflies (Zygoptera) using conventional cytogenetics and fluorescence in situ hybridization: Insights into the karyotype evolution of the ancient insect order Odonata

Kuznetsova

Maryańska-Nadachowska

Anokhin

et al. 2020

J. Zool. Syst. Evol. Res.

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“…Coryphaeschna perrensi (McLachlan, 1887) shows only one GC-rich band in the telomeric region of the largest pair, in this pair a correlation was established between the nucleolar organiser region (NOR) and the GC-rich band (De Gennaro et al 2008). The association between GC-rich bands and NORs is frequent in insects with holokinetic chromosomes (Mola 2007;De Gennaro et al 2008). The GC-rich telomeric band of O. nodiplaga and E. nigricans might correspond to the nucleolus organizer region as well.…”

Section: Heterochromatin Distribution Quantity and Base Pair Richnessmentioning

confidence: 89%

“…Orthemis ambinigra, herein studied, also shows large bands of telomeric heterochromatin with interspersed AT-and GC-rich blocks (equally localized DAPI and CMA 3 bright bands). Orthemis nodiplaga, as well as Erythrodiplax nigricans (Rambur, 1842), has small heterochromatic AT-rich bands in the telomeric region of all chromosomes, except for one GC-rich band in a telomeric region of one pair of chromosomes (De Gennaro et al 2008;this work). Coryphaeschna perrensi (McLachlan, 1887) shows only one GC-rich band in the telomeric region of the largest pair, in this pair a correlation was established between the nucleolar organiser region (NOR) and the GC-rich band (De Gennaro et al 2008).…”

Section: Heterochromatin Distribution Quantity and Base Pair Richnessmentioning

confidence: 99%

“…Orthemis nodiplaga, as well as Erythrodiplax nigricans (Rambur, 1842), has small heterochromatic AT-rich bands in the telomeric region of all chromosomes, except for one GC-rich band in a telomeric region of one pair of chromosomes (De Gennaro et al 2008;this work). Coryphaeschna perrensi (McLachlan, 1887) shows only one GC-rich band in the telomeric region of the largest pair, in this pair a correlation was established between the nucleolar organiser region (NOR) and the GC-rich band (De Gennaro et al 2008). The association between GC-rich bands and NORs is frequent in insects with holokinetic chromosomes (Mola 2007;De Gennaro et al 2008).…”

Section: Heterochromatin Distribution Quantity and Base Pair Richnessmentioning

confidence: 99%

“…In terms of base pair richness, it may be AT-rich or GC-rich, with variations between species, chromosomes of the same species and even within the same chromosome (Grozeva and Marinov 2007;De Gennaro et al 2008;Walia and Katnoria 2017;Walia et al 2018aWalia et al , 2018bWalia and Somal 2019;Walia and Devi 2020).…”

Section: Introductionmentioning

confidence: 99%

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High karyotypic variation in Orthemis Hagen, 1861 species, with insights about the neo-XY in Orthemis ambinigra Calvert, 1909 (Libellulidae, Odonata)

Mola¹,

Fourastié²,

Agopian³

2021

CCG

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The American dragonfly genus Orthemis Hagen, 1861 is mainly found in the Neotropical region. Seven of 28 taxonomically described species have been reported from Argentina. Chromosome studies performed on this genus showed a wide variation in chromosome number and a high frequency of the neoXY chromosomal sex-determination system, although the sexual pair was not observed in all cases. This work analyzes the spermatogenesis of Orthemis discolor (Burmeister, 1839), O. nodiplaga Karsch, 1891 and O. ambinigra Calvert, 1909 in individuals from the provinces of Misiones and Buenos Aires, Argentina. Orthemis discolor has 2n=23, n=11+X and one larger bivalent. Orthemis nodiplaga exhibits the largest chromosome number of the order, 2n=41, n=20+X and small chromosomes. Orthemis ambinigra shows a reduced complement, 2n=12, n=5+neo-XY, large-sized chromosomes, and a homomorphic sex bivalent. Fusions and fragmentations are the main evolutionary mechanisms in Odonata, as well as in other organisms with holokinetic chromosomes. Orthemis nodiplaga would have originated by nine autosomal fragmentations from the ancestral karyotype of the genus (2n=22A+X in males). We argue that the diploid number 23 in Orthemis has a secondary origin from the ancestral karyotype of family Libellulidae (2n=25). The complement of O. ambinigra would have arisen from five autosomal fusions and the insertion of the X chromosome into a fused autosome. C-banding and DAPI/CMA3 staining allowed the identification of the sexual bivalent, which revealed the presence of constitutive heterochromatin. We propose that the chromosome with intermediate C-staining intensity and three medial heterochromatic regions corresponds to the neo-Y and that the neo-system of this species has an ancient evolutionary origin. Moreover, we discuss on the mechanisms involved in the karyotypic evolution of this genus, the characteristics of the neo sex-determining systems and the patterns of heterochromatin distribution, quantity and base pair richness.

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Cytogenetic Characterization of Eight Odonata Species Originating from the Curonian Spit (the Baltic Sea, Russia) Using C-Banding and FISH with 18S rDNA and Telomeric (TTAGG)n Probes

Kuznetsova

Maryańska-Nadachowska²,

Shapoval³

et al. 2017

Cytogenet Genome Res

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We studied the karyotypes of 8 dragonfly species originating from the Curonian Spit (the Baltic Sea, Russia) using C-banding and FISH with 18S rDNA and “insect” telomeric (TTAGG)_n probes. Our results show that Leucorrhinia rubicunda, Libellula depressa, L. quadrimaculata, Orthetrum cancellatum, Sympetrum danae, and S. vulgatum from the family Libellulidae, as well as Cordulia aenea and Epitheca bimaculata from the family Corduliidae share 2n = 25 (24 + X) in males, with a minute pair of m-chromosomes being present in every karyotype except for that of C. aenea. Major rDNA clusters are located on one of the large pairs of autosomes in all the species. No hybridization signals were obtained by FISH with the (TTAGG)_n probe in the examined species with the only exception of S. vulgatum. In this species, clear signals were detected at the ends of almost all chromosomes. This finding raises the possibility that in Odonata the canonical “insect” (TTAGG)_n telomeric repeat is in fact present but in very low copy number and is consequently difficult to detect by in situ hybridization. We conclude that more work needs to be done to answer questions about the organization of telomeres in this very ancient and thus phylogenetically important insect order.

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Fluorescent banding and meiotic behaviour in Erythrodiplax nigricans (Libellulidae) and Coryphaeschna perrensi (Aeschnidae) (Anisoptera, Odonata)

Cited by 8 publications

References 14 publications

Chromosomal analysis of eight species of dragonflies (Anisoptera) and damselflies (Zygoptera) using conventional cytogenetics and fluorescence in situ hybridization: Insights into the karyotype evolution of the ancient insect order Odonata

Chromosomal analysis of eight species of dragonflies (Anisoptera) and damselflies (Zygoptera) using conventional cytogenetics and fluorescence in situ hybridization: Insights into the karyotype evolution of the ancient insect order Odonata

High karyotypic variation in Orthemis Hagen, 1861 species, with insights about the neo-XY in Orthemis ambinigra Calvert, 1909 (Libellulidae, Odonata)

Cytogenetic Characterization of Eight Odonata Species Originating from the Curonian Spit (the Baltic Sea, Russia) Using C-Banding and FISH with 18S rDNA and Telomeric (TTAGG)<sub>n</sub> Probes

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