Fluorescent binary ensemble with pattern recognition ability for identifying multiple metalloproteins with applications in serum and urine

Zheng, Demin; Fan, Junmei; Huang, Xinyan; Ding, Liping; Xin, Yunhong

doi:10.1039/c7ra09741g

Cited by 13 publications

(9 citation statements)

References 41 publications

(47 reference statements)

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“…Another cholic acid-modified pyrene derivative, Monopyrene 5 , carrying a cationic boric acid group was designed as an amphiphilic fluorescent probe . It could also self-assemble into round-shaped aggregates in aqueous solution at a concentration of 5 μM.…”

Section: Applications In Differentiation Of Proteinsmentioning

confidence: 99%

Fluorescent Ensemble Sensors and Arrays Based on Surfactant Aggregates Encapsulating Pyrene-Derived Fluorophores for Differentiation Applications

Qiao

Fan

Ding

et al. 2021

ACS Appl. Mater. Interfaces

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Surfactant assemblies have drawn great attention in fabricating fluorescent sensors as they can provide advantages such as easy preparation, low cost, aqueous detection, high fluorescence stability, high sensitivity to external stimuli, etc. We have devoted the past few years to fluorescent cross-reactive sensors and arrays that are advantageous in differentiating similar analytes and analyzing mixed samples. In this Spotlight on Applications, we introduce our recent advances in developing surfactant assembly-based fluorescent sensors and arrays for discrimination applications. Besides using surfactant assemblies encapsulating fluorophores to fabricate multiple-element-based sensor arrays, we particularly proposed to take advantage of modulation effect of dynamic surfactant assemblies on the photophysical properties of encapsulated fluorophores to construct single-system-based discriminative sensors, which have been successfully applied in differentiation of multiple metal ions and various proteins. The applications of surfactant assembly-based sensors for the detection and discrimination of thiols, amino acids, and explosives are also introduced. Finally, the prospects of further efforts for improving surfactant ensemble sensors and their challenges are discussed.

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Section: Applications In Differentiation Of Proteinsmentioning

confidence: 99%

Fluorescent Ensemble Sensors and Arrays Based on Surfactant Aggregates Encapsulating Pyrene-Derived Fluorophores for Differentiation Applications

Qiao

Fan

Ding

et al. 2021

ACS Appl. Mater. Interfaces

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“…In order to further identify the proteins belong to the same type, we developed a mini sensor array (Cao et al, 2016 ) and a single-sensor based discriminative system, which realized the identification of metalloproteins (Zheng et al, 2017 ). In the single-sensor system, our group have synthesized a mono-pyrene derivative containing a pyridine boronic acid unit to provide glycoprotein receptors and positive charges and a cholic acid group with self-assembly ability to increase the modulation effect by surfactant.…”

Section: Multi-wavelength-based Single Sensing System For Protein Recmentioning

confidence: 99%

“…(B) PCA score plot for identifying metalloproteins at 0.5 μM. Reprinted from Zheng et al ( 2017 ) with permission.…”

Section: Multi-wavelength-based Single Sensing System For Protein Recmentioning

confidence: 99%

Array-Based Discriminative Optical Biosensors for Identifying Multiple Proteins in Aqueous Solution and Biofluids

Fan

Han

et al. 2020

Front. Chem.

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Identification of proteins is an important issue both in medical research and in clinical practice as a large number of proteins are closely related to various diseases. Optical sensor arrays with recognition ability have been flourished to apply for distinguishing multiple chemically or structurally similar analytes and analyzing unknown or mixed samples. This review gives an overview of the recent development of array-based discriminative optical biosensors for recognizing proteins and their applications in real samples. Based on the number of sensor elements and the complexity of constructing array-based discriminative systems, these biosensors can be divided into three categories, which include multi-element-based sensor arrays, environment-sensitive sensor arrays and multi-wavelength-based single sensing systems. For each strategy, the construction of sensing platform and detection mechanism are particularly introduced. Meanwhile, the differences and connections between different strategies were discussed. An understanding of these aspects may help to facilitate the development of novel discriminative biosensors and expand their application prospects.

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“…Chemical structures of probes 33 and 34 and schematic illustration of 34 /SDBS for the cross-reactive sensing of different metalloproteins . Reprinted with permission from ref (copyright 2017, Royal Society of Chemistry).…”

Section: Cross-reactive Sensingmentioning

confidence: 99%

“…As a continuous effort to develop a discriminating sensor for metalloproteins, we built a binary ensemble sensor based on fluorophore 34 and SDBS assemblies (Figure 23). 82 The pyrene-based fluorophore contained a boronic acid group to provide charges and glycoprotein receptors and a cholic acid unit with assembly ability to enhance the modulation effect by surfactants. The fluorescence emission of the probe could be well adjusted from monomer-dominated emission to monomer−excimer coemission by the anionic SDBS surfactants, and the selected binary ensemble exhibited multiple-wavelength cross-reactive responses to seven metalloproteins.…”

Section: Cross-reactive Sensingmentioning

confidence: 99%

Surfactant Aggregates Encapsulating and Modulating: An Effective Way to Generate Selective and Discriminative Fluorescent Sensors

2018

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The heterogeneous structure and dynamic balancing nature of surfactant aggregates make them attractive in developing fluorescent sensors. They can provide a number of advantages, e.g., enhanced fluorescence stability and quantum yield, detection capability in aqueous solutions, and easy operation. Thus, various strategies have been used to construct surfactant aggregate-based fluorescent sensors. Surfactant aggregates play various roles in different strategies and realize multiple sensing behaviors. Many new functions have been discovered for surfactant aggregates in constructing fluorescent sensors. In this feature article, we briefly summarize the development of surfactant aggregate-based fluorescent sensors and their applications in three different types of sensing: selective sensing, multiple analyte sensing, and cross-reactive sensing. For each type of sensing, the design strategies and the roles of surfactant aggregates are particularly introduced. An understanding of these aspects will help to expand the applications of surfactant assemblies in the sensing field.

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Fluorescent binary ensemble with pattern recognition ability for identifying multiple metalloproteins with applications in serum and urine

Abstract: A fluorescent binary ensemble with multiple-wavelength cross-reactivity functioning as a discriminative sensor to identify different metalloproteins in serum or urine solution.

Cited by 13 publications

References 41 publications

Fluorescent Ensemble Sensors and Arrays Based on Surfactant Aggregates Encapsulating Pyrene-Derived Fluorophores for Differentiation Applications

Fluorescent Ensemble Sensors and Arrays Based on Surfactant Aggregates Encapsulating Pyrene-Derived Fluorophores for Differentiation Applications

Array-Based Discriminative Optical Biosensors for Identifying Multiple Proteins in Aqueous Solution and Biofluids

Surfactant Aggregates Encapsulating and Modulating: An Effective Way to Generate Selective and Discriminative Fluorescent Sensors

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