Fluorescent biosensor for alkaline phosphatase based on fluorescein derivatives modified silicon nanowires

Wang, Huimin; Mu, Lixuan; She, Guangwei; Xu, Haitao; Shi, Wensheng

doi:10.1016/j.snb.2014.07.047

Cited by 16 publications

(7 citation statements)

References 44 publications

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“…The SiNWs (by CVD) were mainly utilized for the experiment in vitro. SiNWs (by chemical etching (CE) method) with different lengths can be fabricated by varying the etching time. As shown in Figure S1b–e in the Supporting Information, the diameters of SiNWs range from 100 nm to 400 nm and the lengths of the prepared SiNWs are separately about 30 μm (Figure S1b,c, Supporting Information) and 200 µm (Figure S1d,e, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

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Single Silicon Nanowire‐Based Fluorescent Sensor for Endogenous Hypochlorite in an Individual Cell

Cao

Chen

et al. 2018

Adv. Biosys.

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it is of vital importance to monitor the hypochlorite.So far, many fluorescent probes have been reported for selective signaling of the hypochlorite and they possess great merits such as high sensitivity, fast response time, etc. [5] Owing to some limitations of these research, most sensors would only be able to investigate large numbers of cells. Based on the assumption that the average response represents typical cells within the population, the traditional cellbased assays just measure the average response of cell populations. [6] However, there are significant differences among individuals of the same type of cells. [7] So such average cannot accurately reflect the response from an individual cell and may result in a misleading interpretation. The accurate determination of the hypochlorite at single-cell level could reveal the essence of hypochlorite in life activities and enable physiologists and pathologists to explore the causes of hypochlorite-related diseases and obtain more reliable scientific basis for the development and treatment of the diseases. [8] Thus, it is highly desirable to fabricate a proper fluorescent sensor which could be used to investigate the dynamic activity of the hypochlorite and reveal the biological role of hypochlorite in a single cell.Currently, single-cell study at the nanoscale can present in forms such as pillars, [9] tubes, [10] and wires. [11] These materials as probes are gently inserted into a cell and show great advantages [12] such as active insertion into the cell at the location as we need, realizing subcellular resolution, label-free for a specific location in the cell, decreasing diffusion distance, etc. [13] Modifying a proper molecule on an appropriate anchoring carrier would be a rational strategy to construct sensors for single-cell research. Among the nanomaterials, silicon nanowires (SiNWs) are a competitive alternative as the anchoring substrate. [14] Compared with other nanomaterials, SiNWs own many virtues such as nontoxicity, high stability, easy modification, and praiseworthy biocompatibility. Especially, it can be easily fabricated in good straightness, rigidity, and different lengths. Therefore, it is very favorable to utilize the SiNWs as the anchoring substrate to construct a single nanowire-based sensor for biological detection in a single cell.In this study, we have constructed a single SiNW-based fluorescent sensor for hypochlorite and realized its application in an individual cell with the assistance of micromanipulation and As a prototype of a single-cell detecting technique, a single silicon nanowire-based (SiNW-based) fluorescent sensor for endogenous hypochlorite in a macrophage is constructed. The fluorescent sensor for hypochlorite is prepared by decorating a near-infrared dye, IR780 onto the surface of SiNWs. The sensor exhibits high sensitivity and linear dependence of the fluorescence intensities on the hypochlorite concentrations. With the help of micromanipulation and laser scanning confocal microscopy, detection of endogenous hypoc...

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Section: Resultsmentioning

confidence: 99%

“…The structure and morphologies of SiNWs were recorded by TEM. SiNW arrays were prepared according to silver‐assisted CE method . First, Si (n‐type, ⟨100⟩) strips were cleaned by acetone, ethanol, and deionized water for 10 min separately.…”

Section: Methodsmentioning

confidence: 99%

Single Silicon Nanowire‐Based Fluorescent Sensor for Endogenous Hypochlorite in an Individual Cell

Cao

Chen

et al. 2018

Adv. Biosys.

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“…5b). As normal ALP levels in adults are approximately 20 to 160 U.L −1 [28], which means the developed assay can be applied for the evaluation of ALP activity in human body. The performance of the developed assay was compared with literature reports.…”

Section: Resultsmentioning

confidence: 99%

Monitoring the activity and inhibition of alkaline phosphatase via quenching and restoration of the fluorescence of carbon dots

et al. 2014

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We report that the fluorescence of carbon dots (C-dots) in water is quenched by the addition of Cu 2+ ions, and that the subsequent addition of pyrophosphate (PPi) restores fluorescence. This is likely to be due to the coordination of Cu 2+ by PPi. This effect forms the basis for a method to determine the activity and inhibition of the enzyme alkaline phosphatase (ALP). If ALP is added to a system composed of C-dots, Cu 2+ and PPi, fluorescence will decrease over time because ALP catalyzes the hydrolysis of PPi to form orthophosphate (Pi). This results in a release of the quencher Cu 2+ . The decrease in fluorescence is related to the activity of ALP. The method is simple and displays good sensitivity (with a limit of detection of 1 units per L) and selectivity. The method was successfully applied to the determination of ALP in serum samples. We also have studied the inhibitory effect of Pi on the activity of ALP. We presume that this method holds a large potential in terms of diagnosis of ALP-related diseases, to evaluate the function of ALP in biological systems and in screening for potential inhibitors of ALP.

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“…As far as the fabrication of Si NWs-based fluorescent sensors is concerned, it is possible to generalize 3 types of major Si NW applications as schematized in Figure 1. In particular, (a) Si NWs (or a single Si NW) coupled with fluorescent biomolecules can be employed and used as a substrate [33,34,55,56], (b) metal decorated Si NWs coupled with fluorescent biomolecules can be used as quencher according to the structural conformation of the functionalized material (i.e., quenching when the dye is in close proximitity to gold NP decorated Si NWs) [96,97], (c) light-emitting Si NW directly used as biosensor based on their photoluminescence (PL) quenching [101][102][103]. During the next sections, each one of these interesting strategies will be presented, discussing their major advantages and drawbacks and the type of applications where they have been reported so far.…”

Section: Silicon Nanowire Sensing Platformmentioning

confidence: 99%

“…Another advantage of light-emitting assays is the possibility to be coupled with microscopy analyses improving the sensitivity and spatial resolution and allowing analysis in vivo or inside cell cultures [30][31][32]. Indeed, the development of fluorescent probes that can be precisely located inside the cells is a highly demanded point to investigate certain biomolecule target activity revealing their biological roles [33]. So far, several organic fluorophores have been reported in the literature [34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Biosensors Based on Silicon Nanowires

et al. 2021

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Nanostructures are arising as novel biosensing platforms promising to surpass current performance in terms of sensitivity, selectivity, and affordability of standard approaches. However, for several nanosensors, the material and synthesis used make the industrial transfer of such technologies complex. Silicon nanowires (NWs) are compatible with Si-based flat architecture fabrication and arise as a hopeful solution to couple their interesting physical properties and surface-to-volume ratio to an easy commercial transfer. Among all the transduction methods, fluorescent probes and sensors emerge as some of the most used approaches thanks to their easy data interpretation, measure affordability, and real-time in situ analysis. In fluorescent sensors, Si NWs are employed as substrate and coupled with several fluorophores, NWs can be used as quenchers in stem-loop configuration, and have recently been used for direct fluorescent sensing. In this review, an overview on fluorescent sensors based on Si NWs is presented, analyzing the literature of the field and highlighting the advantages and drawbacks for each strategy.

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Fluorescent biosensor for alkaline phosphatase based on fluorescein derivatives modified silicon nanowires

Cited by 16 publications

References 44 publications

Single Silicon Nanowire‐Based Fluorescent Sensor for Endogenous Hypochlorite in an Individual Cell

Single Silicon Nanowire‐Based Fluorescent Sensor for Endogenous Hypochlorite in an Individual Cell

Monitoring the activity and inhibition of alkaline phosphatase via quenching and restoration of the fluorescence of carbon dots

Fluorescent Biosensors Based on Silicon Nanowires

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