Fluorescent compounds as retrograde tracers compared with horseradish peroxidase (HRP). II. A parametric study in the peripheral motor system of the cat

Illert, M.; Fritz, Norbert; Aschoff, Andreas; Holländer, Horstmar

doi:10.1016/0165-0270(82)90084-x

Cited by 82 publications

(24 citation statements)

References 23 publications

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“…The overall distribution of rhodamine latex beads agrees with previous retrograde studies in our laboratory employing two other fluorescent tracers (Manaker et al, 1992;Manaker and Tischler, 1993), although the current study produced higher absolute numbers of retrogradely labeled neurons using rhodamine latex beads. These differences in absolute numbers of retrogradely labeled neurons may be attributed to the different inherent transport properties of various retrograde tracers (Illert et al, 1982;Craig et al, 1989;Richmond et al, 1994).…”

Section: Discussionmentioning

confidence: 97%

Colocalization of substance P or enkephalin in serotonergic neuronal afferents to the hypoglossal nucleus in the rat

Henry

Manaker

1998

J. Comp. Neurol.

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The serotonergic innervation of the hypoglossal nucleus originates from the caudal raphe nuclei. Non-serotonergic neurons in the caudal raphe nuclei also project to the hypoglossal nucleus. We employed a triple-fluorescence technique to determine whether the substance P- or the enkephalin-containing neurons in the caudal raphe nuclei that projected to the hypoglossal nucleus also contained serotonin. Rhodamine latex microspheres were injected into the hypoglossal nucleus, and then serotonin and peptide dual-immunofluorescence was performed to colocalize perikarya containing serotonin, substance P, and rhodamine microspheres; or perikarya containing serotonin, enkephalin, and rhodamine microspheres. Our results demonstrate that most substance P-containing neuronal afferents to the hypoglossal nucleus colocalize serotonin. In contrast, few enkephalin-containing neuronal afferents to the hypoglossal nucleus also contain serotonin. These data suggest that substance P projections to the hypoglossal nucleus are a subset of serotonergic projections and that limited overlap exists between the populations of enkephalinergic and serotonergic neuronal afferents to the hypoglossal nucleus. Either substance P- or enkephalin-containing somata account for a very small proportion of non-serotonergic caudal raphe projections to the hypoglossal nucleus. Finally, these data demonstrate the medial tegmental field origins of the substance P projections and the enkephalin projections to the hypoglossal nucleus.

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Section: Discussionmentioning

confidence: 97%

Colocalization of substance P or enkephalin in serotonergic neuronal afferents to the hypoglossal nucleus in the rat

Henry

Manaker

1998

J. Comp. Neurol.

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“…Different labelling efficiencies were found in other studies, however. Such differences were found for Fast Blue, propidium iodide, DAPI, bisbenzimide and Nuclear Yellow after application to the cut end of forelimb nerves in the cat (Illert et al 1982), and for True…”

Section: Introductionmentioning

confidence: 86%

“…Fluorescent dyes have also been used to retrogradely label dorsal root ganglia (DRG) cell bodies after injection directly in peripheral tissues such as muscle (Illert et al, 1982;O'Brien, 1989;Buisseret-Delmas, 1990;Richmond et al, 1994), or skin (O'Brien, 1989, but there is little information regarding the relative efficiency of different fluorescent retrograde tracers after cutaneous injection. Such information would be useful for quantitative studies of peripheral skin innervation, e.g.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of the fluorescent dyes Fast Blue, Fluoro-Gold, and Diamidino Yellow for retrograde tracing to dorsal root ganglia after subcutaneous injection

Puigdellı́vol-Sánchez

Prats-Galino

Ruano-Gil

et al. 1998

Journal of Neuroscience Methods

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“…This feature would have permitted the clear detection of double-labeled cells, had they been present. Because FB generally labels 25% fewer cells after a 4-day survival as compared to HRP Illert et al, 1982), our FB counts may be an underestimate of the motor neuron numbers.…”

Section: Fluorescent Tracer Studymentioning

confidence: 98%

Motor pudendal nerve characterization in the female rat

et al. 2001

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The aim of our study was to provide quantitative data on pudendal motor neuron cell bodies and axons in the female rat. To confirm earlier studies, fluorescent retrograde tracers were used to label the motor neurons for correlation with myelinated axon counts along the length of the motor pudendal nerve. The external urethral sphincter of female rats was injected with diamidino yellow and the external anal sphincter with fast blue. The L 6 spinal cord revealed labeled motor neurons. Those in the dorsolateral column (60.8 Ϯ 10.6) had nuclei labeled yellow from the external urethral sphincter and those in the dorsomedial column (31.7 Ϯ 8.5) had cytoplasm labeled blue from the external anal sphincter. Double labeling was not present, suggesting that pudendal motor neurons in each column innervate separate sphincters. The motor pudendal nerve in the ischiorectal fossa was also characterized by light microscopy. The mean myelinated axon count (151.4 Ϯ 17.0) was highly correlated (r ϭ 0.995) in the proximal fascicles and the sum of distal fascicles. This indicated that myelinated axons do not branch at the point where the main motor pudendal nerve branches into separate fascicles. Axon counts between sides were not as well correlated (r ϭ 0.883). The ratio of motor neurons to myelinated axons is 56%, suggesting that some myelinated axons either innervate other muscles or are sensory. This reproducible characterization of the normal pudendal nerve anatomy provides an excellent basis for experimental studies associated with pudendal nerve denervation as a model for neurogenic incontinence.

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Fluorescent compounds as retrograde tracers compared with horseradish peroxidase (HRP). II. A parametric study in the peripheral motor system of the cat

Cited by 82 publications

References 23 publications

Colocalization of substance P or enkephalin in serotonergic neuronal afferents to the hypoglossal nucleus in the rat

Colocalization of substance P or enkephalin in serotonergic neuronal afferents to the hypoglossal nucleus in the rat

Efficacy of the fluorescent dyes Fast Blue, Fluoro-Gold, and Diamidino Yellow for retrograde tracing to dorsal root ganglia after subcutaneous injection

Motor pudendal nerve characterization in the female rat

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