Fluorescent detection of target proteins via a molecularly imprinted hydrogel

Abstract

Proteins are typically separated by immune-reaction, such as enzyme-linked immuno sorbent assay, and detected by certain fluorescent labeling. They have usually potential of complicated procedures, denaturation of proteins by labeling,...

Customizable molecular recognition: advancements in design, synthesis, and application of molecularly imprinted polymers

“…Fluorescent detection of proteins was investigated when researchers Kubo et al detected bovine serum albumin (BSA) using protein imprinted hydrogels (PI gels) composed of poly(ethylene glycol)(PEG). 112 The fluorescent dye, 8-anilino-1-napthalenesulfonic acid (ANS), was incorporated into a PEG-containing monomer prior to polymerization to assist in observing the efficiency of the PI gels. The ANS moiety was found to selectively interact with the hydrophobic part in BSA due to the imprint effect.…”

Specific recognition of a target protein, cytochrome c, using molecularly imprinted hydrogels

“…Thus, it is worthy to study the PEG-based MIPs to promote the diversity of MIP materials. Until now, the PEG-based hydrogels have found applications for carbohydrates, proteins, and small molecules, by showing specific adsorption, 37 retention/affinity effect for separation, 19 fluorescent response, 48 and swelling-deswelling behavior 49 to the targets.…”