Fluorescent immunochromatography of vancomycin in plasma: A rapid and sensitive quantitative analysis

Abstract

Vancomycin (VAN) has a narrow therapeutic window and variable pharmacokinetic properties, which require timely monitoring of plasma concentration to adjust the dosage for better effectiveness. A sensitive and quantitative immunochromatographic method was developed to detect VAN in plasma. The linear response range of the method to detect plasma VAN was 1.25-50 μg/mL. The intra-and inter-assay coefficients were 4.68% and 8.81%, respectively, while the recovery was 89.10%-98.32%. The clinical comparative experiment results demonstrated a good correlation (r > 0.90) between the fluorescent immunochromatographic strip test and the mass spectrum. In this study, a simple, rapid, and high-sensitivity immunochromatographic method was established for detecting VAN, which helped establish the basis for further application of monitoring plasma concentration.

One-Step Detection of Vancomycin in Whole Blood Using the Lateral Flow Immunoassay

“…LFIAs have advantages such as its simple procedure, time efficiency, low cost, easy interpretation of results, and a wide detection range [ 21 , 22 ]. Therefore, various studies have been conducted to detect VAN using LFIAs, providing reliable detection results from samples such as milk, serum, and plasma, demonstrating the outstanding advantages of LFIAs [ 23 , 24 , 25 ]. However, the LFIA method used in these studies had limitations, such as requiring multiple steps during sample preparation or detection.…”

“…For the plasma sample, pre-treatment was required to separate blood cells from blood samples, and before using strips, VAN antibodies were fluorescently labeled, and the fluorescently labeled VAN antibody (Ab) served as the detection solution. The detection solution was subsequently mixed with a diluted plasma sample, and then the mixed solution was loaded onto the strip, requiring a two-step detection process for the strip [ 25 ]. These procedures make the whole detection process complex, requiring supplementary equipment and extended time for analysis.…”