Fluorescent In Situ Hybridization (FISH)

Ramos, Jeanette M

doi:10.1007/978-1-0716-1948-3_12

Cited by 8 publications

(3 citation statements)

References 6 publications

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“…Techniques for characterising individual cells at the molecular level in their natural spatial context are advancing rapidly. Recently, conventional experimental procedures like DNA barcoding, 12 immunohistochemistry (IHC) for protein detection, 13 and fluorescent in situ hybridisation (FISH) for nucleic acid detection 14 have paved the way for spatial omics technologies to encompass a broader range of targets and/or regions. Spatial omics technologies capture a range of properties, with consideration for spatial resolution, coverage, scale, throughput and multiplexing capacity required depending on the scientific question being asked.…”

Section: Spatial Profiling Techniquesmentioning

confidence: 99%

Redefining clinical practice through spatial profiling: a revolution in tissue analysis

Mulholland,

Leedham

2024

annals

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Spatial biology, which combines molecular biology and advanced imaging, enhances our understanding of tissue cellular organisation. Despite its potential, spatial omics encounters challenges related to data complexity, computational requirements and standardisation of analysis. In clinical applications, spatial omics has the potential to revolutionise biomarker discovery, disease stratification and personalised treatments. It can identify disease-specific cell patterns, and could help risk stratify patients for clinical trials and disease-appropriate therapies. Although there are challenges in adopting it in clinical practice, spatial omics has the potential to significantly enhance patient outcomes. In this paper, we discuss the recent evolution of spatial biology, and its potential for improving our tissue level understanding and treatment of disease, to help advance precision and effectiveness in healthcare interventions.

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Section: Spatial Profiling Techniquesmentioning

confidence: 99%

Redefining clinical practice through spatial profiling: a revolution in tissue analysis

Mulholland,

Leedham

2024

annals

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“…In situ hybridization (ISH) is an experimental method for locating specific nucleic acid sequences in cell or tissue sections. The main steps include preparing a probe (usually a labeled piece of DNA or RNA that can specifically pair with the target sequence), hybridization (the pairing process between the probe and the target sequence), washing [6].…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Research on the function of CG15382 by mutating Zelda binding region

Chang

2024

TNS

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Zelda protein (Zld) is a transcription factor in drosophila. It binds on specific sequence to activate the expression of genes involved in the early development of drosophila, which hinted a method to study the function of these genes. Since the expression of genes can be regulated by Zld, mutation of Zld binding site can be used to silence target genes. Three base pairs were substituted by using CRISPR-Cas9 editing. The function of target genes in the development process can be studied by comparing wild type drosophila and the Zld absent mutant (Zld-). This paper will focus on CG15382 and discuss the design of the mutant drosophila.

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“…17 In addition, mRNA expression in tumour tissue sections was examined by the SweAMI FISH assay. 18 The probe sequence for the SweAMI FISH assay is summarized in Table S3. The probes for the SweAMI FISH assay were labelled with the fluorescence dye CY3 and equally mixed for target mRNA examination.…”

Section: Immunohistochemistry Staining and Sweami Fish Assaymentioning

confidence: 99%

Decreased RNA‐binding protein heterogeneous nuclear ribonucleoprotein U improves multiple myeloma sensitivity to lenalidomide

Lin,

Zhang,

Liu

et al. 2024

Br J Haematol

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SummaryMultiple myeloma (MM) is an incurable plasma cell cancer in the bone marrow. Immunomodulatory drugs, such as lenalidomide (LEN) and pomalidomide, are backbone agents in MM treatment, and LEN resistance is commonly seen in the MM clinic. In this study, we presented that heterogeneous nuclear ribonucleoprotein U (hnRNPU) affected MM resistance to LEN via the regulation of target mRNA translation. hnRNPULow MM cells exhibited upregulated CRBN and IKZF1 proteins, stringent IKZF1/3 protein degradation upon LEN addition and increased sensitivity to LEN. RNA pulldown assays and RNA electrophoretic mobility shift assays revealed that hnRNPU bound to the 3′‐untranslated region of CRBN and IKZF1 mRNA. A sucrose gradient assay suggested that hnRNPU specifically regulated CRBN and IKZF1 mRNA translation. The competition of hnRNPU binding to its target mRNAs by small RNAs with hnRNPU‐binding sites restored MM sensitivity to LEN. hnRNPU function in vivo was confirmed in an immunocompetent MM mouse model constructed by the inoculation of Crbn‐humanized murine 5TGM1 cells into CrbnI391V/+ mice. Overall, this study suggests a novel mechanism of LEN sensitivity in which hnRNPU represses CRBN and IKZF1 mRNA translation.

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Fluorescent In Situ Hybridization (FISH)

Cited by 8 publications

References 6 publications

Redefining clinical practice through spatial profiling: a revolution in tissue analysis

Redefining clinical practice through spatial profiling: a revolution in tissue analysis

Research on the function of CG15382 by mutating Zelda binding region

Decreased RNA‐binding protein heterogeneous nuclear ribonucleoprotein U improves multiple myeloma sensitivity to lenalidomide

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