Fluorescent labeling of oleanolic acid using ‘click’ chemistry

Abstract

The natural product oleanolic acid (OA) was functionalized and labeled with the non-aggregated fluorescent dye BOPIM (boron 2-(2′-pyridyl)imidazole complex) using ‘click’ chemistry, yielding a highly fluorescent probe. The pharmacological activity of the probe is comparable to that of unmodified OA, making it suitable for studying the therapeutic mechanisms of OA derivatives in vitro.

Natural-product-based fluorescent probes: recent advances and applications

“…64 Fluorescent derivative 61 labeled with boron 2-(20-pyridyl)imidazole (BOPIM) was prepared. 65 Evaluation of its cardioprotective effects on primary neonatal rat cardiomyocytes with hypoxia/reoxygenation (H/R) injuries showed significant prevention of H/R-induced cell death. 64 Thus, BOPIM-OA 61 is a promising fluorescent probe for investigating the pharmacological mechanisms of natural products.…”