Fluorescent magnetic bead-based mast cell biosensor for electrochemical detection of allergens in foodstuffs

Jiang, Donglei; Zhu, Pei‐Wen; Jiang, Hui; Ji, Jian; Sun, Xiulan; Gu, Wenshu; Zhang, Genyi

doi:10.1016/j.bios.2015.03.058

Cited by 60 publications

(30 citation statements)

References 33 publications

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“…However, these nanoparticles (MNPs) are more prone to agglomerate and to suffer losses during their more challenging handling than their micrometric analogues [ 15 , 16 ]. In electrochemical biosensing approaches, MNPs have been used mainly as nanosurfaces where affinity conjugation takes place without the need for applying costly and complicated modification procedures to the electrode platforms [ 17 , 18 , 19 , 20 , 21 , 22 , 23 ], but also as catalytic label and as nanocarriers of signalling molecules.…”

Section: Selected Nanostructures In Electrochemical Affinity Biosementioning

confidence: 99%

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

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The excellent capabilities demonstrated over the last few years by electrochemical affinity biosensors should be largely attributed to their coupling with particular nanostructures including dendrimers, DNA-based nanoskeletons, molecular imprinted polymers, metal-organic frameworks, nanozymes and magnetic and mesoporous silica nanoparticles. This review article aims to give, by highlighting representative methods reported in the last 5 years, an updated and general overview of the main improvements that the use of such well-ordered nanomaterials as electrode modifiers or advanced labels confer to electrochemical affinity biosensors in terms of sensitivity, selectivity, stability, conductivity and biocompatibility focused on food and environmental applications, less covered in the literature than clinics. A wide variety of bioreceptors (antibodies, DNAs, aptamers, lectins, mast cells, DNAzymes), affinity reactions (single, sandwich, competitive and displacement) and detection strategies (label-free or label-based using mainly natural but also artificial enzymes), whose performance is substantially improved when used in conjunction with nanostructured systems, are critically discussed together with the great diversity of molecular targets that nanostructured affinity biosensors are able to quantify using quite simple protocols in a wide variety of matrices and with the sensitivity required by legislation. The large number of possibilities and the versatility of these approaches, the main challenges to face in order to achieve other pursued capabilities (development of antifouling, continuous operation, wash-, calibration- and reagents-free devices, regulatory or Association of Official Analytical Chemists, AOAC, approval) and decisive future actions to achieve the commercialization and acceptance of these devices in our daily routine are also noted at the end.

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Section: Selected Nanostructures In Electrochemical Affinity Biosementioning

confidence: 99%

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

View full text Add to dashboard Cite

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“…The mast cells have a natural ability to determine the presence of the food allergens and further produce proinflammatory responses. These offer an accurate and stable strategy mimicking the physiological conditions [108]. A new paper-based analytical device has been reported for the sensitive detection of casein [73].…”

Section: Immunosensorsmentioning

confidence: 99%

Nano-Biosensing Platforms for Detection of Cow’s Milk Allergens: An Overview

Nehra

Lettieri

Dilbaghi

et al. 2019

Sensors

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Among prevalent food allergies, cow milk allergy (CMA) is most common and may persist throughout the life. The allergic individuals are exposed to a constant threat due to milk proteins’ presence in uncounted food products like yogurt, cheese, and bakery items. The problem can be more severe due to cross-reactivity of the milk allergens in the food products due to homologous milk proteins of diverse species. This problem can be overcome by proper and reliable food labeling in order to ensure the life quality of allergic persons. Therefore, highly sensitive and accurate analytical techniques should be developed to detect the food allergens. Here, significant research advances in biosensors (specifically immunosensors and aptasensors) are reviewed for detection of the milk allergens. Different allergic proteins of cow milk are described here along with the analytical standard methods for their detection. Additionally, the commercial status of biosensors is also discussed in comparison to conventional techniques like enzyme-linked immunosorbent assay (ELISA). The development of novel biosensing mechanisms/kits for milk allergens detection is imperative from the perspective of enforcement of labeling regulations and directives keeping in view the sensitive individuals.

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“…However, it is difficult to perform on-site detection, or use as a high-throughput screen because of the complicated sample pre-treatment procedures, expensive equipment, and trained personnel needed. Furthermore, various biosensors exhibiting high sensitivity and good specificity have been designed and developed to determine shrimp tropomyosin (Jiang et al, 2013;Jiang et al, 2015;Jiang et al, 2019;Tabrizi, Shamsipur, Saber, Sarkar, & Ebrahimi, 2017;Wang, Rao, Zhou, Zheng, & Fu, 2019). However, it also requires sophisticated sample preparation steps and expensive equipment.…”

Section: Introductionmentioning

confidence: 99%

Development of a sandwich ELISA and immunochromatographic strip for the detection of shrimp tropomyosin

Zeng

Song

Zheng³

et al. 2019

Food and Agricultural Immunology

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Shrimp tropomyosin is one of the most important causes of shellfish allergy. The objective of this study was to develop a highly sensitive and specific immunoassay for the detection of shrimp tropomyosin in food. Ten monoclonal antibodies against shrimp tropomyosin were obtained by fusion and cell screening. We found two optimum monoclonal antibodies (mAbs); mAb 2 was used as the capture antibody, and HRP-labelled mAb 3 was used as the detection antibody. Using this pair of mAbs, a sandwich enzymelinked immunosorbent assay (ELISA) was developed with the limit of detection (LOD) of 0.45 ng/mL. Cross-reactivity with other food allergens using this method was found to be negligible. An immunochromatographic assay strip was also developed for the rapid detection of shrimp tropomyosin with the LOD of 0.5 ng/ mL. The results demonstrated that our developmental methods represent a useful tool for the detection of shrimp tropomyosin in food.

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Fluorescent magnetic bead-based mast cell biosensor for electrochemical detection of allergens in foodstuffs

Cited by 60 publications

References 33 publications

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Nano-Biosensing Platforms for Detection of Cow’s Milk Allergens: An Overview

Development of a sandwich ELISA and immunochromatographic strip for the detection of shrimp tropomyosin

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