Fluorescent Mueller matrix analysis of a highly scattering turbid media

Abstract

We report the fluorescent Mueller matrix analysis of a highly scattering, inhomogeneous, and low quantum yield polymeric nanoparticle system. Both the ground and the excited state anisotropy of this turbid system were measured. The excited state anisotropy was found to be higher than ground state anisotropy by inverse polar decomposition analysis. The depolarization coefficients of these polythiophene nanoparticles were experimentally determined by recording Mueller matrices from this complex random medium. This approach provides an alternative method of determining optical characteristics of low quantum efficiency turbid system like fluorescently leveled tissue phantom.

Polarisation optics for biomedical and clinical applications: a review

“…Here we briefly summarise common phantoms used for biomedical polarimetric techniques. These techniques include polarised wide-field microscopy 16 , 24 , 183 , polarised light spatial frequency imaging 184 , polarimetric endoscopy 185 – 190 , spectral light scattering polarimetry 18 , 82 , 191 – 193 , polarised fluorescence spectroscopy 194 – 196 , polarised confocal microscopy 197 , polarised Raman-spectroscopy 198 , 199 , polarised super-resolution microscopy 154 , 155 , polarisation sensitive optical coherence tomography 200 – 218 , non-diffraction beam polarimetry (such as Bessel beam based) 219 , polarisation-resolved nonlinear microscopy (including second/third harmonic generation) 220 – 226 , and polarised speckle imaging 213 , 227 (several techniques will be mentioned again in the Discussion). The relationship between incoherence and depolarisation of the light should be kept in mind when considering coherence based polarimetric techniques: they are different but related optical concepts.…”

Safeguarding long-lived excitons from excimer traps in H-aggregated dye-assemblies

“…The experiments were performed on a single mX-PBI microcrystal in exact backscattering collection geometry, following a method developed by Ghosh and coworkers. 24 , 25 The elements of the 4 × 4 matrix were derived from sixteen separate measurements using different combinations of incident and emission polarization states (see the ESI † for details), and are presented in Fig. 3a .…”

Optically tunable engineered macromolecular fluorescent material analyzed using optical parameters

“…Traditional fluorescence polarization parameters extract contained partial information, which was found to be moderately successful. The polarization parameters—Fluorescence Diattenuation and Polarizance derived from fluorescence Mueller matrix (FLMM) quantifies the anisotropy associated with fluorescence excitation and emission separately 18–25 …”