Fluorescent naphthalimide boronates as theranostics: structural investigations, confocal fluorescence and multiphoton fluorescence lifetime imaging microscopy in living cells

Green, Megan J.; Ge, Haobo; Flower, Stephen E.; Pourzand, Charareh; Botchway, Stanley W.; Wang, Hui-Chen; Kuganathan, Navaratnarajah; Kociok-Köhn, Gabriele; Li, Meng; Xu, Suying; James, Tony D.; Pascu, Sofia I.

doi:10.1039/d3cb00112a

Cited by 3 publications

References 54 publications

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Tripodal BODIPY‐Tagged and Functional Molecular Probes: Synthesis, Computational Investigations and Explorations by Multiphoton Fluorescence Lifetime Imaging Microscopy

Lledos,

Calatayud,

Cortezon‐Tamarit

et al. 2024

Chemistry A European J

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A range of novel BODIPY derivatives with a tripodal aromatic core was synthesized and characterized spectroscopically. These new fluorophores showed promising features as probes for in vitro assays in live cells and offer strategic routes for further functionalization towards hybrid nanomaterials. Incorporation of biotin tags facilitated proof‐of‐concept access to targeted bioconjugates as molecular probes. Computational explorations using DFT and TD‐DFT calculations identified the most stable tripodal linker conformations and predicted their absorption and emission behavior. The uptake and speciation of these molecules in living prostate cancer cells was imaged by single‐ and two‐photon excitation techniques coupled with two‐photon fluorescence lifetime imaging (2P FLIM)

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Tripodal BODIPY‐Tagged and Functional Molecular Probes: Synthesis, Computational Investigations and Explorations by Multiphoton Fluorescence Lifetime Imaging Microscopy

Lledos,

Calatayud,

Cortezon‐Tamarit

et al. 2024

Chemistry A European J

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Recent advances in dual response molecular probes for biothiols

Mwafulirwa,

Abdalla,

Bian

et al. 2024

Smart Molecules

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The detection of biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are critical for understanding their roles in biology and their involvement in various physiological and pathological processes. Recently, significant progress has been made in constructing fluorescent probes capable of detecting and visualizing biothiols. This review provides an in‐depth look at the latest advancements in simultaneous and selective molecular probes, focusing on developments over the last 5 years. We examine design techniques, sensing mechanisms, and imaging methods to assess their effectiveness and responsiveness to thiols. Additionally, we discuss the prevailing challenges and offer recommendations to address them.

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Multiphoton FLIM and PLIM: Emerging Imaging and Sensing Tools for Probing Metal Complexes and Nanohybrid Materials in Complex Cellular Environments

Calatayud,

Arrowsmith,

Waghorn

et al. 2024

Imaging Tools for Chemical Biology

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Fluorescence and phosphorescence lifetime imaging microscopies (FLIM and PLIM) have found applications at the interface between life and physical sciences with relevance to biomedicine. This chapter outlines a facet of the broader optical imaging landscape, by exploring the applications of FLIM and PLIM that can help elucidate the fate of molecular probes when located within specific cellular environments. We highlight several case studies of advances in microscopy using FLIM and PLIM, with a focus on the detection of chemical probes within living cells, encompassing selected recent published work over the past decade and with a particular focus on metal complexes and related molecular materials, including carbon-based nanoparticles. We also aim to show how variations in fluorescence lifetimes can be applied to gain a deeper understanding of molecular interactions in the quest to pinpoint both static and dynamic cellular processes, as well as the cellular environment. There are prospects in the applications of the technology in the near-infrared (NIR) spectrum: this extension of current lifetime imaging capabilities by employing detectors and probes suited to address the NIR technologies has several advantages and could revolutionize the biomedical imaging, sensing and diagnostics domains, by enabling deeper tissue penetration and mitigating autofluorescence. This approach to imaging NIR-emitting probes using lifetime imaging could open new avenues for cutting-edge research and applications at the interface between physical, life and biomedical sciences.

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Fluorescent naphthalimide boronates as theranostics: structural investigations, confocal fluorescence and multiphoton fluorescence lifetime imaging microscopy in living cells

Abstract: New design and synthetic strategies were developed to generate functional phenyl boronic acid (BA)-based fluorescent probes incorporating the 1,8-naphthalimide (NI) tag. This fluorescent core was anchored onto the BA unit...

Cited by 3 publications

References 54 publications

Tripodal BODIPY‐Tagged and Functional Molecular Probes: Synthesis, Computational Investigations and Explorations by Multiphoton Fluorescence Lifetime Imaging Microscopy

Tripodal BODIPY‐Tagged and Functional Molecular Probes: Synthesis, Computational Investigations and Explorations by Multiphoton Fluorescence Lifetime Imaging Microscopy

Recent advances in dual response molecular probes for biothiols

Multiphoton FLIM and PLIM: Emerging Imaging and Sensing Tools for Probing Metal Complexes and Nanohybrid Materials in Complex Cellular Environments

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