Fluorescent probe 4-dimethylaminochalcone: Solvatochromism according to the data of semiempirical and quantum-chemical calculations

Bakhshiev, N. G.; Gularyan, S. K.; Dobretsov, G. E.; Kirillova, A. Yu.; Svetlichnyĭ, V. Yu.

doi:10.1134/s0030400x09030138

Cited by 5 publications

(2 citation statements)

References 7 publications

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“…As a result, the energy-related parameters of probe fluorescence show nonlinear dependence upon the total concentration of the active component in the mixture. 64 Such phenomenon is known as the preferential solvation effect. 64,65 Such preferential solvation of the probe by water or by the organic solvent result in substantial upward and downward deviations from the linearity in the dependence of log(N*/T*) as a function of the total water concentration (Supporting Information, Figure S4).…”

Section: Calibration Of Probementioning

confidence: 99%

“…64 Such phenomenon is known as the preferential solvation effect. 64,65 Such preferential solvation of the probe by water or by the organic solvent result in substantial upward and downward deviations from the linearity in the dependence of log(N*/T*) as a function of the total water concentration (Supporting Information, Figure S4). For instance, the strong HB-donor water molecules dominate over acetonitrile in the solvation shell (Supporting Information, Figure S4 upward deviations), while lipophilic 2-propanol and, especially, tetrahydrofurane dominate over water (Supporting Information, Figure S4, downward deviations).…”

Section: Calibration Of Probementioning

confidence: 99%

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Quantification of Local Hydration at the Surface of Biomolecules Using Dual-Fluorescence Labels

et al. 2012

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By using four labels of the 3-hydroxyflavone family displaying selective sensitivity to hydrogen bond (HB) donors and poor response to other polar molecules, we developed an approach for measuring local water concentration [H(2)O](L) (or partial volume of water: W(A) = [H(2)O](L)/55.6) in the label surrounding both in solvent mixtures and in biomolecules by the intensity ratio of two emissive forms of the label, N*/T*. Using a series of binary water/solvent mixtures with limited preferential solvation effects, a linear dependence of log(N*/T*) on the local concentration of HB donor was obtained and then used as a calibration curve for estimating the W(A) values in the surroundings of the probes conjugated to biomolecules. By this approach, we estimated the hydration of the labels in different peptides and their complexes with DNAs. We found that W(A) values for the label at the peptide N-terminus are lower (0.63-0.91) than for free labels and depend strongly on the nature of the N-terminal amino acid. When complexed with different DNAs, the estimated hydration of the labels conjugated to the labeled peptides was much lower (W(A) = 0-0.47) and depended on the DNA nature and linker-label structure. Thus, the elaborated method allows a site-specific evaluation of hydration at the surface of a biomolecule through the determination of the partial volume of water. We believe the developed procedure can be successfully applied for monitoring hydration at the surface of any biomolecule or nanostructure.

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Section: Calibration Of Probementioning

confidence: 99%