Fluorescent probes - illuminate the interplay network between lipid droplets and other organelles

Yang, Jialu; Ning, Juan; Sun, Pengju; Nie, Yamin; Li, Yonghong; Guo, Meixia; Zhou, Yanmei

doi:10.1016/j.ccr.2024.215792

Cited by 12 publications

(1 citation statement)

References 135 publications

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“…Lipid droplets (LDs), which serve as the primary storage sites for intracellular neutral lipids, are complex, active, and highly dynamic multifunctional organelles. They play important roles in intracellular energy storage and metabolism, membrane transport, protein degradation, and signaling. − A variety of metabolic diseases, including nonalcoholic fatty liver disease, cardiovascular disease, and type II diabetes, are often accompanied by the dysregulation of intracellular lipid homeostasis. − The nuclear membrane (NM) is a bilayered structure enveloping the cell nucleus that is composed of lipids, proteins, and small amounts of sugars and is involved in many key biological processes in cells, including gene regulation, DNA replication, and RNA transport . Similarly, the NM plays a vital role in the formation of LDs.…”

Section: Introductionmentioning

confidence: 99%

3D Imaging of Lipid Droplet–Nuclear Membrane Contact Sites and Cirrhotic Lipid Droplet Overexpression

Ma,

Huang,

Long

et al. 2024

Anal. Chem.

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To coordinate cellular physiology, cells rely on the rapid exchange of molecules at specialized organelle–organelle contact sites. Lipid droplets (LDs) and nuclear membrane (NM) contact sites are particularly vital communication hubs, playing key roles in the exchange of signaling molecules, lipids, and metabolites. However, there is still a lack of understanding of the specific morphology of the contact sites. Here, we combine advanced three-dimensional (3D) imaging with a high-brightness fluorescent probe specifically targeting LDs to map the structural landscape of LD–NM contact sites. The probe exhibits exceptional photophysical properties, making it highly suitable for visualizing the changes occurring in LDs during the apoptosis process. In addition, we utilize the advantages of the probe to accurately monitor the overexpression of abnormal LDs in cirrhosis by 3D imaging for the first time. The outcomes of this investigation highlight that the probe has potential as a robust imaging tool to investigate intricate biological functions of LDs and their implications in related diseases.

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Section: Introductionmentioning

confidence: 99%

3D Imaging of Lipid Droplet–Nuclear Membrane Contact Sites and Cirrhotic Lipid Droplet Overexpression

Ma,

Huang,

Long

et al. 2024

Anal. Chem.

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Clog P‐Guided Development of Multi‐Colored Buffering Fluorescent Probes for Super‐Resolution Imaging of Lipid Droplet Dynamics

Chen,

Qiao,

Wang

et al. 2024

Advanced Science

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Super‐resolution fluorescence imaging of live cells increasingly demands fluorescent probes capable of multi‐color and long‐term dynamic imaging. Understanding the mechanisms of probe‐target recognition is essential for the engineered development of such probes. In this study, it is discovered that the molecular lipid solubility parameter, Clog P, determines the staining performance of fluorescent dyes on lipid droplets (LDs). Fluorescent dyes with Clog P values between 2.5 and 4 can form buffering pools outside LDs, replacing photobleached dyes within LDs to maintain constant fluorescence intensity in LDs, thereby enabling dynamic super‐resolution imaging of LDs. Guided by Clog P, four different colored buffering LD probes spanning the visible light spectrum have been developed. Using Structured Illumination Microscopy (SIM), the role of LD dynamics have been tracked during cellular ferroptosis with the secretion, storage, and degradation of overexpressed ACSL3 proteins. It is found that LDs serve as storage sites for these proteins through membrane fusion, and further degrade overexpressed proteins via interactions with organelles like lysosomes or through lipophagy, thereby maintaining cellular homeostasis.

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Recent advances in single fluorescent probes for monitoring dual organelles in two channels

Yang,

Yao,

et al. 2024

Smart Molecules

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Organelles are specialized areas where cells perform specific processes necessary for life and actively communicate with each other to keep the whole cell functioning. Disorders of the organelle networks are associated with multiple pathological processes. However, clearly and intuitively visualizing the highly dynamic interactions between ultrafine organelles is challenging. Fluorescence imaging technology provides opportunities due to the distinct advantages of facile, non‐invasiveness and dynamic detection, making it particularly well‐suited for applications in uncovering the mysterious veil of organelle interactions. Regrettably, the lack of ideal fluorescence agents has always been an obstacle in imaging the intricate behaviors of organelles. In this review, we provide a systematic discussion on the existing dual‐color and dual‐targetable molecular sensors used in monitoring organelle interactions, with a specific focus on their targeting strategies, imaging mechanisms and biological applications. Additionally, the current limitations and future development directions of dual‐targetable probes and dual‐emissives are briefly discussed. This review aims to provide guidance for researchers to develop more improved probes for studying organelle interactions in the biomedical field.

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Fluorescent probes - illuminate the interplay network between lipid droplets and other organelles

Cited by 12 publications

References 135 publications

3D Imaging of Lipid Droplet–Nuclear Membrane Contact Sites and Cirrhotic Lipid Droplet Overexpression

3D Imaging of Lipid Droplet–Nuclear Membrane Contact Sites and Cirrhotic Lipid Droplet Overexpression

Clog P‐Guided Development of Multi‐Colored Buffering Fluorescent Probes for Super‐Resolution Imaging of Lipid Droplet Dynamics

Recent advances in single fluorescent probes for monitoring dual organelles in two channels

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