Fluorescent protein marker lines in maize: generation and applications

Wu, Qingyu; Luo, Anding; Zadrozny, Tara; Sylvester, Anne W.; Jackson, D. I.

doi:10.1387/ijdb.130240qw

Cited by 35 publications

(28 citation statements)

References 101 publications

(112 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, our results showed that the expression level of auxin-responsive genes was significantly higher in DSY2 as compared with DSY79, suggesting modulating auxin-signaling might be one strategy that DSY2 applied to cope with P-starvation. Therefore, further studies may focus on analyzing auxin distribution transport using the maize fluorescence-based reporter lines from the maize cell genomic resources1415. Besides the auxin, recent studies also report that the cell wall proteins expansins that regulate cell division and expansion rates may play a critical role in the root architecture responses to low-P stress1116.…”

Section: Discussionmentioning

confidence: 99%

Revealing new insights into different phosphorus-starving responses between two maize (Zea mays) inbred lines by transcriptomic and proteomic studies

Jiang

Zhang

Han

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

Phosphorus (P) is an essential plant nutrient, and deficiency of P is one of the most important factors restricting maize yield. Therefore, it is necessary to develop a more efficient program of P fertilization and breeding crop varieties with enhanced Pi uptake and use efficiency, which required understanding how plants respond to Pi starvation. To understand how maize plants adapt to P-deficiency stress, we screened 116 inbred lines in the field and identified two lines, DSY2 and DSY79 that were extreme low-P resistant and sensitive, respectively. We further conducted physiological, transcriptomic, and proteomic studies using the roots of DSY2 and DSY79 under normal or low-P conditions. The results showed that the low-P resistant line, DSY2 had larger root length, surface area and volume, higher root vitality, as well as acid phosphatase activity as compared with the low-P sensitive line, DSY79 under the low-P condition. The transcriptomic and proteomic results suggest that dramatic more genes were induced in DSY2, including the plant hormone signaling, acid phosphatase, and metabolite genes, as compared with DSY79 after being challenged by low-P stress. The new insights generated in this study will be useful toward the improvement of P-utilize efficiency in maize.

show abstract

Section: Discussionmentioning

confidence: 99%

Revealing new insights into different phosphorus-starving responses between two maize (Zea mays) inbred lines by transcriptomic and proteomic studies

Jiang

Zhang

Han

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Thus, it cannot be ruled out that ZmLOX10 provides substrate towards JA biosynthesis during stress response in a tissue- or stimulus-specific manner. A cautionary note, careful examination of the subcellular localization of ZmLOX9-YFP, did not find it to be localized to chloroplasts [55]. …”

Section: 13-lipoxygenasesmentioning

confidence: 99%

Synthesis and Functions of Jasmonates in Maize

Borrego

Kolomiets

2016

Plants

109

View full text Add to dashboard Cite

Of the over 600 oxylipins present in all plants, the phytohormone jasmonic acid (JA) remains the best understood in terms of its biosynthesis, function and signaling. Much like their eicosanoid analogues in mammalian system, evidence is growing for the role of the other oxylipins in diverse physiological processes. JA serves as the model plant oxylipin species and regulates defense and development. For several decades, the biology of JA has been characterized in a few dicot species, yet the function of JA in monocots has only recently begun to be elucidated. In this work, the synthesis and function of JA in maize is presented from the perspective of oxylipin biology. The maize genes responsible for catalyzing the reactions in the JA biosynthesis are clarified and described. Recent studies into the function of JA in maize defense against insect herbivory, pathogens and its role in growth and development are highlighted. Additionally, a list of JA-responsive genes is presented for use as biological markers for improving future investigations into JA signaling in maize.

show abstract

“…In addition to these traditional genetic resources, there is a large collection of fluorescent protein marker lines (Mohanty et al 2009; Wu et al 2013). Among these is a suite of cell biological markers (tubulin, chromosomes, ER, cell wall, etc .)…”

Section: Chromosomes and Mutant Collectionsmentioning

confidence: 99%

“…These lines are being used in live-cell studies of cell division (Figure 3B) and, for example, to interpret the expression of a gene that suppresses branching (tillering) at the base of the plant thereby promoting the single-stalk growth pattern typical of agricultural corn (Whipple et al 2011). Two-component trans -activating fluorescent lines have also been generated for tissue-specific control of gene expression (Wu et al 2013). …”

Section: Chromosomes and Mutant Collectionsmentioning

confidence: 99%

Genetic and Genomic Toolbox of Zea mays

Nannas

Dawe

2015

Genetics

View full text Add to dashboard Cite

Maize has a long history of genetic and genomic tool development and is considered one of the most accessible higher plant systems. With a fully sequenced genome, a suite of cytogenetic tools, methods for both forward and reverse genetics, and characterized phenotype markers, maize is amenable to studying questions beyond plant biology. Major discoveries in the areas of transposons, imprinting, and chromosome biology came from work in maize. Moving forward in the post-genomic era, this classic model system will continue to be at the forefront of basic biological study. In this review, we outline the basics of working with maize and describe its rich genetic toolbox. KEYWORDS genetics; genomics; maize; toolsWorking with Maize T HE term maize is often used synonymously with corn, particularly in the United States and in reference to its agricultural use. While both terms are correct, maize is a name that refers uniquely to this plant. Maize is a large grain plant that evolved from its wild-grass ancestors by the direct intervention of human agriculture. Many varieties or "races" differ in physical properties (Goodman and Brown 1988), but generally maize is a single-stalk plant that grows to approximately 8 feet tall with about 20 long, narrow leaves growing individually from nodes along the stalk (Figure 1A) (Kiesselbach 1999). Several characteristics make it an attractive genetic system (Strable and Scanlon 2009). It is easy to culture on any scale, from a few plants in pots to many acres ( Figure 1B). It can be grown successfully year round in greenhouses and growth chambers with proper lighting; it is also quite hardy and can be grown outdoors under a range of conditions, from tropical to temperate climates (Shaw 1988). Maize is a naturally outcrossing species, which makes its genetic architecture (diversity, linkage, recombination, etc.) more similar to other outcrossing organisms such as humans rather than self-pollinating plants ( Rafalski and Morgante 2004;Wallace et al. 2013). While its genetics are similar to humans, maize retains the major strength of plant genetics: the ability to self-cross and quickly produce homozygotes or F2 populations.The male and female reproductive organs are accessible and separable, making controlled crosses easy to perform. The male germ cells are produced in the tassel found at the top of the plant ( Figure 1C). Tassels contain anthers that open upon maturation, releasing up to 10 7 wind-dispersed pollen grains (Coe et al. 1988). The female germ cells are located in one or more ears, which grow from the base of leaves in the midsection of the plant (Figure 1, A and D). An ear generally contains several hundred egg cells that will develop into kernels after fertilization (Neuffer et al. 1997). Each young kernel contains a silk, an elongated stigma, which emerges out of the husk leaves of the ear ( Figure 1D). Pollen grains land on the silk and produce a pollen tube that grows down through the length of the silk, ultimately delivering two sperm to the female gametophy...

show abstract

Fluorescent protein marker lines in maize: generation and applications

Cited by 35 publications

References 101 publications

Revealing new insights into different phosphorus-starving responses between two maize (Zea mays) inbred lines by transcriptomic and proteomic studies

Revealing new insights into different phosphorus-starving responses between two maize (Zea mays) inbred lines by transcriptomic and proteomic studies

Synthesis and Functions of Jasmonates in Maize

Genetic and Genomic Toolbox of Zea mays

Contact Info

Product

Resources

About